Differential expression of the psbB and psbH genes encoding the 47 kDa chlorophyll a-protein and the 10 kDa phosphoprotein of photosystem II during chloroplast development in wheat

Hird, Sean M.; Webber, Andrew N.; Wilson, Rebecca J.; Dyer, Tristan A.; Gray, John C.

doi:10.1007/bf00336487

Cited by 29 publications

(14 citation statements)

References 62 publications

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“…On one hand, the Chl a/b-proteins of PSII show a lower ratio of Chl to protein and a higher Chl-a/b ratio. As repeatedly shown, Chl is necessary for the stabilization of most Chl-protein complexes (Mathis and Burkey 1989;Henry et al 1990;Hoober et al 1990;Mullet et al 1990;Hird et al 1991). However, as our data indicate, the full setup is not required.…”

Section: Discussionsupporting

confidence: 38%

“…Chlorophyll, however, seems to be necessary for stabilizing a number of Chl-binding apoproteins of the PSII core-complex (Mullet etal. 1990;Hird et al 1991) as well as the light-harvesting complex II (LHCII; Mathis and Burkey 1989;Henry et al 1990;Hoober et al 1990). These proteins are not found in dark-grown plants and in plants from IL the size of the Chl-antenna is reduced (Argyroudi-Akoyunoglou and Akoyunoglou 1979; Dubertret and LefortTran 1981;Day et al 1984;White and Green 1988;Morrissey et al 1989).…”

Section: Introductionmentioning

confidence: 98%

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Chlorophyll-proteins from maize seedlings grown under intermittent light conditions

Marquardt

Bassi

1993

Planta

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Abstract.We studied the organization of the antenna system of maize (Zea mays L.) seedlings grown under intermittent light conditions for 11 d. These plants had a higher chlorophyll-a/b ratio, a higher ratio of carotenoids to chlorophyll and a lower ratio of chlorophyll to protein than plants grown in continuous light. We found all chlorophyll-protein complexes of maize to be present. However, the minor chlorophyll a/b-proteins CP29 and CP26, and to a greater extent CP24 and the major light-harvesting complex II were reduced relative to the photosystem (PS) II core-complex. Also the chlorophyll a/b-antennae of PSI were reduced relative to the reaction-centre polypeptides. When isolated by flatbed isoelectrofocussing, the chlorophyll-a/b complexes of PSII showed a higher chlorophyll-a/b ratio and a lower ratio of chlorophyll to protein than the same complexes from continuous light; additionally, they bound more carotenoids per protein than the latter. Thus the altered organization of the photosynthetic apparatus of plants from intermittent light is caused by two different factors: (i) the altered stoichiometry of chlorophyll-binding proteins and (ii) a different ratio of pigment to protein within individual chlorophyll-proteins.

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Section: Discussionsupporting

confidence: 38%

Section: Introductionmentioning

confidence: 98%

Chlorophyll-proteins from maize seedlings grown under intermittent light conditions

Marquardt

Bassi

1993

Planta

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“…Their total lengths were 14.0 and 13.7 kb, respectively. The ctDNA regions included the 3Ј flanking region of the Rubisco large subunit gene (rbcL) (10) and 10 structural genes: encod-ing ATP synthase, ␣, ␤, and subunits (atpA, atpB, and atpE) (11), ATP synthase, CF 0 subunit III and CF 0 subunit I (atpH and atpF) (12), the 47-kDa chlorophyll a-binding protein, the 10-kDa phosphoprotein of photosystem II, photosystem II polypeptide of 43 aa, apocytochrome b-563, and the 15-kDa polypeptide of the cytochrome b-f complex (psbB, psbN, psbH, petB, and petD) (13). The mtDNA regions include nine structural genes: encoding the cytochrome oxidase subunit I, II, and III (cox1, cox2, and cox3) (14-16), the ATP synthase ␣ subunit (atpA) (17), NADH dehydrogenase subunit I (nad1-a, nad1-b, and nad1-e) (18), cytochrome b (cob) (19), and ORF25 (orf25) (20).…”

Section: Methodsmentioning

confidence: 99%

Plasmon analyses of Triticum (wheat) and Aegilops : PCR–single-strand conformational polymorphism (PCR-SSCP) analyses of organellar DNAs

Wang

Miyashita

Tsunewaki

1997

Proc. Natl. Acad. Sci. U.S.A.

116

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To investigate phylogenetic relationships among plasmons in Triticum and Aegilops, PCR-single-strand conformational polymorphism (PCR-SSCP) analyses were made of 14.0-kb chloroplast (ct) and 13.7-kb mitochondrial (mt)DNA regions that were isolated from 46 alloplasmic wheat lines and one euplasmic line. These plasmons represent 31 species of the two genera. The ct and mtDNA regions included 10 and 9 structural genes, respectively. A total of 177 bands were detected, of which 40.6% were variable. The proportion of variable bands in ctDNA (51.1%) was higher than that of mtDNA (28.9%). The phylogenetic trees of plasmons, derived by two different models, indicate a common picture of plasmon divergence in the two genera and suggest three major groups of plasmons (Einkorn, Triticum, and Aegilops). Because of uniparental plasmon transmission, the maternal parents of all but one polyploid species were identified. Only one Aegilops species, Ae. speltoides, was included in the Triticum group, suggesting that this species is the plasmon and B and G genome donor of all polyploid wheats. ctDNA variations were more intimately correlated with vegetative characters, whereas mtDNA variations were more closely correlated with reproductive characters. Plasmon divergence among the diploids of the two genera largely paralleled genome divergence. The relative times of origin of the polyploid species were inferred from genetic distances from their putative maternal parents.Genetic diversity among plasmons within two genera, Triticum and Aegilops, was first reported by Kihara (1). On producing alloplasmic lines of common wheat (2n ϭ 6x ϭ 42, nuclear genome AABBDD), and then tracking plasmon-specific phenotypic variations, we were able to classify the plasmons of Triticum and Aegilops species into 16 types (2). The next logical step was to identify molecular variation of their organellar DNAs. First, the discovery of RFLP (restriction fragment length polymorphism) variation among ct and mtDNAs was reported (3). Second, physical maps of common wheat ctDNA were constructed by using three restriction enzymes (4). The ctDNA maps were refined by using 13 restriction enzymes, after which we discovered that the chloroplast genomes of 33 Triticum and Aegilops species fell into 16 types (5). Eventually, RFLP analyses of mtDNAs from 17 species allowed us to distinguish their mitochondrial genomes from each other (6). Even though sequencing analyses are not as thorough as the RFLP analyses, the sequence of one chloroplast gene (rbcL, for the Rubisco large subunit) from seven Triticum and Aegilops species indicated that Ae. speltoides is the donor of both the plasmon and B genome of common wheat (7).Although RFLP and sequencing analyses have been employed, sequencing lags behind. RFLP analyses are relatively easy but are insensitive to fine-structure variation. Sequencing is the ultimate way to detect variation, but it is cumbersome when applied to large numbers of species and DNA regions. This paper reports a new technique, PCR-single-stran...

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“…The PsbH polypeptide was present in both etiolated tissues and deetiolated leaves, whereas the PsbB subunit was absent from dark-grown wheat plants but became detectable on greening. Phosphorylation of the PsbH polypeptide occurred even in etioplasts (Hird et al 1991).…”

Section: Psbh Subunitmentioning

confidence: 99%

Chloroplast-encoded small subunits of the three multiprotein complexes in photosynthetic electron transport

Takahashi

1998

J. Plant Res.

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The photosystem I, photosystem II, and cytochrome bsf complexes that are involved in electron transport of oxygenic photosynthesis consist of a number of subunits encoded by either the chloroplast or nuclear genomes. In addition to the major subunits that carry redox components or photosynthetic pigments, these complexes contain several to more than ten subunits with molecular masses of less than 10 kDa. Directed mutagenesis has served as a powerful tool for investigation of the roles of these small subunits in the organization or function of the complexes.

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Differential expression of the psbB and psbH genes encoding the 47 kDa chlorophyll a-protein and the 10 kDa phosphoprotein of photosystem II during chloroplast development in wheat

Cited by 29 publications

References 62 publications

Chlorophyll-proteins from maize seedlings grown under intermittent light conditions

Chlorophyll-proteins from maize seedlings grown under intermittent light conditions

Plasmon analyses of Triticum (wheat) and Aegilops : PCR–single-strand conformational polymorphism (PCR-SSCP) analyses of organellar DNAs

Chloroplast-encoded small subunits of the three multiprotein complexes in photosynthetic electron transport

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