Differential expression of surface glycoconjugates on Entamoeba histolytica and Entamoeba dispar

Pacheco‐Yépez, Judith; Campos-Rodrı́guez, Rafael; Rojas-Hernández, Saúl; Serrano-Luna, José J.; Rivera-Aguilar, Víctor; Villa‐Treviño, Saúl; Martı́nez-Palomo, Adolfo; Tsutsumi, Vı́ctor; Shibayama, Mineko

doi:10.1016/j.parint.2009.02.003

Cited by 9 publications

(7 citation statements)

References 46 publications

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“…In addition, we also found a large amount of O ‐Glycosylations. About carbohydrates, there are reports that have shown that these participate in a very important way during the protozoan invasion process 69‐71 . In vitro experiments have shown that the trophozoites of N fowleri have high levels of glycoconjugates on their surfaces, mainly α‐ d ‐glucose, α‐ d ‐mannose, N ‐acetylneuraminic acid and terminal α‐L‐fucose residues, 26,72,73 and in vivo experiments have demonstrated the possibility that some of the glycoproteins found in N fowleri represent a factor of adherence to host cells 27 .…”

Section: Discussionmentioning

confidence: 99%

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Gutiérrez‐Sánchez

Carrasco-Yépez

Herrera‐Díaz

et al. 2020

Parasite Immunology

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Many pathogenicity factors are involved in the development of primary amoebic meningoencephalitis (PAM) caused by N fowleri. However, most of them are not exclusive for N fowleri and they have not even been described in other nonpathogenic Naegleria species. Therefore, the objective of this work was to identify differential proteins and protein pattern recognition between Naegleria fowleri and Naegleria lovaniensis using antibodies anti‐N fowleri as strategy to find vaccine candidates against meningoencephalitis. Electrophoresis and Western blots conventional and 2‐DE were performed for the identification of antigenic proteins, and these were analysed by the mass spectrometry technique. The results obtained in 2‐DE gels and Western blot showed very notable differences in spot intensity between these two species, specifically those with relative molecular weight of 100, 75, 50 and 19 kDa. Some spots corresponding to these molecular weights were identified as actin fragment, myosin II, heat shock protein, membrane protein Mp2CL5 among others, with differences in theoretical post‐translational modifications. In this work, we found differences in antigenic proteins between both species, proteins that could be used for a further development of vaccines against N fowleri infection.

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Section: Discussionmentioning

confidence: 99%

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Gutiérrez‐Sánchez

Carrasco-Yépez

Herrera‐Díaz

et al. 2020

Parasite Immunology

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“…Several amoebic species like Entamoeba histolytica , Entamoeba hartmanni , Entamoeba coli , Iodamoeba bütschlii , Dientamoeba fragilis , Endolimax nana and Entamoeba dispar can colonize the human gut, however only E. histolytica is able to cause tissue damage. Comparison of transcriptomic, proteomic, biochemical and in vivo analyses of E. histolytica and E. dispar suggests that the inability of the latter to invade tissues is related to decreased capacities for adhesion, cytotoxicity, capping of surface receptors and proteolysis (Nickel et al ., ; Willhoeft et al ., ; Pacheco‐Yépez et al ., ; Christy and Petri, ; Chávez‐Munguía et al ., ). During infection and tissue invasion, E. histolytica is exposed to oxidant environments.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of intracellular hypoxia and adequate heat shock response are essential requirements for pathogenicity and virulence ofEntamoeba histolytica

et al. 2015

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SummaryAdhesion to cells, cytotoxicity and proteolysis are functions required for virulence and pathogenicity of Entamoeba histolytica. However, there was no correlation between these in vitro functions and the early elimination of non-pathogenic E. dispar and non-virulent E. histolytica (nvEh) in experimental amoebic liver abscesses developed in hamsters. Thus, additional functions may be involved in amoebic pathogenicity and virulence. In the present study, an integral experimental assessment, including innovative technologies for analyses of amoebal pathophysiology, cell biology, biochemistry and transcriptomics, was carried out to elucidate whether other cellular processes are involved in amoebal pathogenicity and virulence. In comparison with virulent E. histolytica, the data indicated that the main reasons for the early clearance of nvEh from hamster liver are decreased intracellular H 2O2 detoxification rate and deficient heat shock protein expression, whereas for E. dispar, it is a relatively lower capacity for O2 reduction. Therefore, maintenance of an intracellular hypoxic environment combined with the induction of an adequate parasite response to oxidative stress are essential requirements for Entamoeba survival in the liver, and therefore for pathogenicity.

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“…As a working hypothesis, we might consider that the lack of capacity of the non-invasive E. dispar to displace and eliminate surface antigen-antibody complexes here shown could render this amoeba susceptible to the humoral immune response of the host and could be related, at least partially, to our previous finding on the failure of this amoeba to produce liver abscesses in experimental models, in contrast to the liver lesions produced under similar conditions by the invasive E. histolytica (Pacheco-Yépez et al 2009). Recently, differences in cellular motility found between the two species of amoebas have also been considered as possible factors in the invasive nature of E. histolytica (Zaki et al 2006).…”

Section: Discussionmentioning

confidence: 76%

“…For this reason, few laboratories have been able to study the latter parasite under axenic conditions. Our group has previously demonstrated that the two species of intestinal amoebas differ in morphology, ultrastructure, surface properties, expression of surface glycoconjugates and in vitro and in vivo cytopathic effect (Espinosa-Cantellano et al 1998;Pacheco-Yépez et al 2009). The non-invasive character of E. dispar has been corroborated in experimental intestinal models (Bansal et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Differences in cap formation between invasive Entamoeba histolytica and non-invasive Entamoeba dispar

Chávez‐Munguía¹,

Talamás-Rohana²,

Castañón³

et al. 2012

Parasitol Res

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The rapid redistribution of surface antigen-antibody complexes in trophozoites of the human protozoan parasite Entamoeba histolytica, in a process known as capping, has been considered as a means of the parasite to evade the host immune response. So far, capping has been documented in the invasive E. histolytica, whereas the mobility of surface components in the non-invasive Entamoeba dispar is not known. E. dispar does not induce liver lesions in rodent experimental models, in contrast to the liver abscesses produced by E. histolytica in the same animal model. We have therefore analyzed the mobility of surface receptors to the lectin concanavalin A and of Rab11, a membrane-associated protein, in both species of Entamoebae by confocal fluorescence microscopy and transmission and scanning electron microscopy. The great majority of E. histolytica trophozoites became morphologically polarized through the formation of well-defined caps at the posterior pole of the parasite. Actin colocalized with the lectin caps. Antibodies against the membrane protein Rab 11 also produced capping. In striking contrast, in E. dispar, the mobility of concanavalin A surface receptors was restricted to the formation of irregular surface patches that did no progress to constitute well-defined caps. Also, anti-Rab 11 antibodies did not result in capping in E. dispar. Whether the failure of E. dispar to efficiently mobilize surface molecules in response to lectin or antibodies as shown in the present results is related to its non-invasive character represents an interesting hypothesis requiring further analysis.

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Differential expression of surface glycoconjugates on Entamoeba histolytica and Entamoeba dispar

Cited by 9 publications

References 46 publications

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Maintenance of intracellular hypoxia and adequate heat shock response are essential requirements for pathogenicity and virulence ofEntamoeba histolytica

Differences in cap formation between invasive Entamoeba histolytica and non-invasive Entamoeba dispar

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