Differential expression of succinyl CoA transferase (SCOT) genes in somatic and germline cells of the mouse testis

Tanaka, Hiromitsu; Iguchi, Naoko; Miyagawa, Yasushi; Koga, Minoru; Kohroki, Junya; Nishimune, Yoshitake

doi:10.1046/j.1365-2605.2003.00389.x

Cited by 18 publications

(21 citation statements)

References 16 publications

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“…It plays a role in energy metabolism of sperm (Koga et al, 2000). SCOT-t has a 2.5-fold higher activity that SCOT-s activity in Leydig cells (Tanaka et al, 2003). A human form has been identified and is a single intronless gene specifically found in the midpiece of the tail .…”

Section: Mitochondriamentioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

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As germ cells divide and differentiate from spermatogonia to spermatozoa, they share a number of structural and functional features that are common to all generations of germ cells and these features are discussed herein. Germ cells are linked to one another by large intercellular bridges which serve to move molecules and even large organelles from the cytoplasm of one cell to another. Mitochondria take on different shapes and features and topographical arrangements to accommodate their specific needs during spermatogenesis. The nuclear envelope and pore complex also undergo extensive modifications concomitant with the development of germ cell generations. Apoptosis is an event that is normally triggered by germ cells and involves many proteins. It occurs to limit the germ cell pool and acts as a quality control mechanism. The ubiquitin pathway comprises enzymes that ubiquitinate as well as deubiquitinate target proteins and this pathway is present and functional in germ cells. Germ cells express many proteins involved in water balance and pH control as well as voltage-gated ion channel movement. In the nucleus, proteins undergo epigenetic modifications which include methylation, acetylation, and phosphorylation, with each of these modifications signaling changes in chromatin structure. Germ cells contain specialized transcription complexes that coordinate the differentiation program of spermatogenesis, and there are many male germ cell-specific differences in the components of this machinery. All of the above features of germ cells will be discussed along with the specific proteins/genes and abnormalities to fertility related to each topic.

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Section: Mitochondriamentioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

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show abstract

“…, 2000; Tanaka et al. , 2001a), substituting for ubiquitously expressed SCOT (somatic cell type) during the differentiation of germ cells (Tanaka et al. , 2003).…”

Section: Introductionmentioning

confidence: 99%

Ketone bodies could support the motility but not the acrosome reaction of mouse sperm

et al. 2004

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Ketone bodies, D-beta-hydroxybutyrate and acetoacetate, produced by the metabolism of fatty acids, are an important energy source for many organs, especially the heart, kidney and brain. They are utilized by the body with the help of succinyl CoA transferase (SCOT), which is ubiquitously expressed in various organs. Previously, we identified a novel SCOT-t specifically expressed in testicular germ cells and sperm, substituting somatic cell-type SCOT, however the physiological role of SCOT-t had not then been clarified. In the present study, we investigated the effects of ketone bodies, the substrate of SCOT-t, on the motility and acrosome reaction of mouse sperm. D-beta-hydroxybutyrate and acetoacetate both stimulated the motility of sperm as glucose or pyruvate. The glycolysis inhibitor stopped the motility of sperm mediated by glucose but not by D-beta-hydroxybutyrate. In contrast, ketone bodies did not stimulate the activation of the acrosome reaction of sperm, different from the effect of glucose. These results indicate that ketone bodies could be involved in sperm movement but not the acrosome reaction and the SCOT-t enzyme we have identified in sperm mitochondria may have important roles in the activity of sperm, resulting in male infertility when its function is disabled.

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“…Expression of mouse SCOT-t in haploid spermatids was confirmed by Northern blot, in situ hybridization, and Western blot analyses (Koga et al, 2000). Differential expression of SCOT genes in somatic and germline cells of mouse testis was also observed (Tanaka et al, 2003). Somatic SCOT (SCOT-s) mRNA expression was observed in mouse kidney, heart, and brain, whereas mouse SCOT-t was detected in the testis.…”

Section: Resultsmentioning

confidence: 83%

“…Therefore, SCOT is an indispensable factor for energy production using ketone bodies. SCOT cDNA sequences have been previously identified from mouse kidney, rabbit brain, as well as pig and human heart tissues (Carninci et al, 2000;Ganapathi et al, 1987;Kassovska-Bratinova et al, 1996;Lin and Bridger, 1992;Tanaka et al, 2003). The cDNA and amino acid sequences of OXCT1 gene show high similarity with those of human and pig (90.1% and 92.8%, respectively) (KassovskaBratinova et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Identification of ORF sequences and exercise-induced expression change in thoroughbred horse OXCT1 gene

Nam

Ahn

Bae

et al. 2012

Gene

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Differential expression of succinyl CoA transferase (SCOT) genes in somatic and germline cells of the mouse testis

Cited by 18 publications

References 16 publications

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Ketone bodies could support the motility but not the acrosome reaction of mouse sperm

Identification of ORF sequences and exercise-induced expression change in thoroughbred horse OXCT1 gene

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