Differential expression of stromal MMP-1, MMP-9 and TIMP-1 in basal cell carcinomas of immunosuppressed patients and controls

Abstract

Matrix metalloproteinases (MMPs) have an important role in the initiation, growth, and invasion of malignant tumors. Basal cell cancer (BCC) is the most common human malignancy. The risk of BCC is 10-16 times higher among organ transplant recipients compared with the nontransplanted population. The aim of this study was to compare the expression of several MMPs and their tissue inhibitors (TIMPs) in BCCs from kidney transplant recipients and controls. Expression of MMPs-1, -7, -8, -9, -10, -13, -26, and TIMPs-1 and -3 was evaluated by immunohistochemistry in 25 samples of BCC of kidney transplant recipients and 25 matched controls representing superficial and nodular subtypes. No significant differences were detected in MMP expression of BCC tumor cells between immunocompetent and immunodeficient patients. However, MMPs-1 and -9 and TIMP-1 were expressed more frequently in stromal macrophages in the BCCs of immunocompetent patients. When tumor subtypes were compared irrespective of the patient group, more MMP-1-positive fibroblasts and MMP-9-positive neutrophils were detected in the superficial subtype, while stromal MMP-10 expression was more abundant in nodular tumors. Our results suggest that abundant peritumoral expression of TIMP-1 in non-immunocompromised patients limits ECM degradation permissive for cancer cell migration.

Immunohistochemical expression of cathepsin D and matrix metalloproteinase-9 in common epidermal tumors

“…This result was also in agreement with that of Boyd et al [27]. In the current study, there was a statistically significant relationship between MMP-9 expression and the histopathological grade of SCC cases.…”

“…Kerkelä et al [25,26] previously reported that stromal cells are the major source of MMPs. Boyd et al [27] reported that MMP-9 was not detected in the BCC epithelium, but in neutrophils and macrophages surrounding it. Whether this strong stromal Well-differentiated squamous cell carcinoma with intense stromal and epithelial positivity for matrix metalloproteinase-9 (MMP-9) (immunoperoxidase for MMP-9, Â 100).…”

Mechanisms of collective cell migration at a glance

“…Such basement-membrane deposition is an early event during wound healing of the skin, whereby a keratinocyte monolayer moves across provisional wound matrix and deposits a basement membrane in cooperation with dermal fibroblasts (Friedl and Gilmour, 2009). Likewise, in epithelial cancers such as oral cancer and basal cell carcinoma, collective invasion occurs along an intact basement membrane, yet the role of the basement membrane in favouring or counteracting collective invasion is not clear (Bauer et al, 2008;Boyd et al, 2008).…”

Expression of MMP-2, MMP-9, MMP-14, TIMP-1, TIMP-2 in Intraepithelial and Invasive Cervical Neoplasia

“…Tumor cells secrete MMPs and induce stromal cells to produce MMPs [4,5]. MMP-2 and MMP-9 are proteolytic enzymes with a major role in type IV collagen digestion, an important event in vascular invasion and metastasis.…”

“…TIMPs are multifunctional proteins that not only inhibit MMPs but also promote cell growth, induce apoptosis, and inhibit angiogenesis [5,7]. However evidence suggests that TIMPs, in particular TIMP-1 and TIMP-2, may have unique biological properties, independent of their ability to inhibit MMPs [8].…”