Differential expression of SofDIR16 and SofCAD genes in smut resistant and susceptible sugarcane cultivars in response to Sporisorium scitamineum

Sánchez-Elordi, Elena; Contreras, Roberto; Armas, Roberto de; Benito, C.; Alarcón, Borja; Oliveira, Eliandre de; Mazo, Carlos del; Díaz-Peña, Eva M.; Santiago, Rocío; Vicente, Carlos; Legaz, Marı́a Estrella

doi:10.1016/j.jplph.2018.04.016

Cited by 13 publications

(10 citation statements)

References 48 publications

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“…As inferred from the qRT-PCR data, in case of watermelon, ClDIR02, 5 and 8 exhibited constitutive higher expression in resistant cultivars, which is much similar to the finding of Elena Sánchez-Elord et al, in which the higher transcript level was observed in resistant cultivars [65]. SofDIR16 gene from sugarcane was highly expressed in resistant cultivar after inoculation of Sporisorium scitamineum [65]. On the other hand, the similar patterns were observed from melon heat map analysis, certain DIR genes, including CmDIR01, 6, 7, 12, and 15 were highly upregulated in resistant cultivar.…”

Section: Cultivar Specific Inducible Expression Pattern Of Cldir and supporting

confidence: 87%

“…According to our expression assessment outcomes, the expression level of DIRs in plant tissues during the disease developmental stage reflects their feasible direct or indirect active participation in different stages of infection. As inferred from the qRT-PCR data, in case of watermelon, ClDIR02, 5 and 8 exhibited constitutive higher expression in resistant cultivars, which is much similar to the finding of Elena Sánchez-Elord et al, in which the higher transcript level was observed in resistant cultivars [65]. SofDIR16 gene from sugarcane was highly expressed in resistant cultivar after inoculation of Sporisorium scitamineum [65].…”

Section: Cultivar Specific Inducible Expression Pattern Of Cldir and supporting

confidence: 86%

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Comparative Analysis, Characterization and Evolutionary Study of Dirigent Gene Family in Cucurbitaceae and Expression of Novel Dirigent Peptide against Powdery Mildew Stress

Yadav

Wang

Yang

et al. 2021

Genes

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Dirigent (DIR) proteins are induced under various stress conditions and involved in sterio- and regio-selective coupling of monolignol. A striking lack of information about dirigent genes in cucurbitaceae plants underscores the importance of functional characterization. In this study, 112 DIR genes were identified in six species, and 61 genes from major cultivated species were analyzed. DIRs were analyzed using various bioinformatics tools and complemented by expression profiling. Phylogenetic analysis segregated the putative DIRs into six distinctively known subgroups. Chromosomal mapping revealed uneven distribution of genes, whereas synteny analysis exhibited that duplication events occurred during gene evolution. Gene structure analysis suggested the gain of introns during gene diversification. Gene ontology (GO) enrichment analysis indicates the participation of proteins in lignification and pathogen resistance activities. We also determined their organ-specific expression levels in three species revealing preferential expression in root and leaves. Furthermore, the number of CmDIR (CmDIR1, 6, 7 and 12) and ClDIR (ClDIR2, 5, 8, 9 and 17) genes exhibited higher expression in resistant cultivars after powdery mildew (PM) inoculation. In summary, based on the expression and in-silico analysis, we propose a role of DIRs in disease resistance mechanisms.

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Section: Cultivar Specific Inducible Expression Pattern Of Cldir and supporting

confidence: 87%

Section: Cultivar Specific Inducible Expression Pattern Of Cldir and supporting

confidence: 86%

Comparative Analysis, Characterization and Evolutionary Study of Dirigent Gene Family in Cucurbitaceae and Expression of Novel Dirigent Peptide against Powdery Mildew Stress

Yadav

Wang

Yang

et al. 2021

Genes

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“…Another model of interaction between individuals, studied in our laboratory, is the pathosystem Saccharum officinarum-Sporisorium scitamineum. Plant invasion by the pathogen causes the production of at least 5-6 defense proteins, among which a dirigent protein [14], secreted arginase, β-1,3-and β-1,4-glucanases, chitinase as well as a sixth protein that acts as a positive chemotactic factor have been identified [15]. The actions that these secreted proteins carry out on the spores of the pathogen are varied.…”

Section: Secreted Proteinsmentioning

confidence: 99%

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Sánchez-Elordi¹,

Díaz²,

Vicente³

et al. 2020

Parasitology and Microbiology Research

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This study demonstrates the involvement of the cytoskeleton in the movement of cyanobacteria and fungal spores to their hosts to establish a state of symbiosis or pathogenicity. The term symbiosis sensu lato is referred not only to commensalism and mutualism but also to the parasitic aberrations. The establishment of association implies that the endohabitant can move on a wet surface until finding an entry point in the exohabitant surface. In aqueous media, the exohabitant secretes glycoproteins that form a chemoattraction gradient for the invading cells. In lichens, the gradient consists of fungal lectins whose function is to recognize a compatible green alga or cyanobacterium. In the case of pathogens, the secreted proteins usually are a mixture that includes false quorum and chemoattractant signals, and cell wall digestive enzymes. The results indicate that fungal lectins and defense proteins bind to specific cell wall receptors for signaling the activation of cytoskeleton, causing successive cycles of cell contraction-relaxation that permits the migration of the endohabitant. In this study, different biochemical and microscopy techniques have been used. The mechanisms through which the cytoskeleton carries out these cycles of cell contractionrelaxation are described, being this a remarkable advance compared to previous results.

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“…It is speculated that ESB1 interacts with a lignin-specific peroxidase in order to guide it into the proper position for lignin polymerization. Additionally, a correlation between dirigent protein expression and lignin accumulation has been observed in many studies [127,130,[270][271][272] suggesting that dirigent proteins are involved in lignification in tissues other than the root endodermis, where the Casparian strip is formed. Therefore, the DE dirigent protein transcripts, in this study, could control lignin composition by directing the localization and polymerization of lignin in the culm, similar to ESB1, and further studies should investigate this finding by creating sugarcane plants with silenced ShDIR1-like expression.…”

Section: Tentative Monolignol Transport and Polymerization Genes Associated With S/g Ratiomentioning

confidence: 83%

“…Recently, several studies have used RNA-seq in sugarcane to discover genes associated with biomass traits, which may be promising candidates for genetic engineering and molecular breeding. In these studies, trait-associated genes are identified as those that are differentially expressed (based on relative transcript abundance) between genotypes with contrasting phenotypes [121][122][123][124][125][126][127][128].…”

Section: Using Transcriptomic Analyses To Identify Genes Associated With Biomass Traitsmentioning

confidence: 99%

Analysis of biomass traits in sugarcane (Saccharum spp. hybrids)

Hodgson‐Kratky¹

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Sugarcane is a C4 perennial grass able to efficiently convert light energy into biomass. Bagasse, the fibrous waste product of sugar production, and biomass from high-fiber sugarcane varieties are important feedstocks for replacing fossil carbon in the production of fuels and other chemicals.Enzymatic hydrolysis can be used to convert the cellulose and hemicellulose components of the biomass into fermentable sugars. These sugars can then be fermented into ethanol and other chemicals using microbial organisms and catalysts. However, enzymatic hydrolysis is inefficient due to the recalcitrant nature of the secondary cell wall structure in which the cellulose and hemicellulose are located. Costly pretreatments are required to break down this structure prior to enzymatic hydrolysis

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Differential expression of SofDIR16 and SofCAD genes in smut resistant and susceptible sugarcane cultivars in response to Sporisorium scitamineum

Cited by 13 publications

References 48 publications

Comparative Analysis, Characterization and Evolutionary Study of Dirigent Gene Family in Cucurbitaceae and Expression of Novel Dirigent Peptide against Powdery Mildew Stress

Comparative Analysis, Characterization and Evolutionary Study of Dirigent Gene Family in Cucurbitaceae and Expression of Novel Dirigent Peptide against Powdery Mildew Stress

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Analysis of biomass traits in sugarcane (Saccharum spp. hybrids)

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