Differential Expression of Six Rnase2 and Three Rnase3 Paralogs Identified in Blunt Snout Bream in Response to Aeromonas hydrophila Infection

Geng, Ruijing; Liu, Han; Wang, Weimin

doi:10.3390/genes9020095

Cited by 6 publications

(4 citation statements)

References 54 publications

(74 reference statements)

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“…In addition, yeast RNaseT2 combines its enzymatic action with other non-catalytic properties such as binding to regulatory proteins and the destabilization of lysosomal membrane, a mechanism that can trigger the programmed cell death ( 56 , 252 ). Overall, we observed common properties between the RNaseA and the RNaseT2 family members, e.g., release of stress-induced tiRNA, leukocyte activation, or exRNA scavenging ( 30 , 31 , 56 , 250 ).…”

Section: Other Extracellular Rnasesmentioning

confidence: 77%

“…Interestingly, ZF-RNases 1 to 3 can activate the ERK1/2 kinase pathway similar to hRNase5 ( 249 ). Recently, the expression of several RNase2 and RNase3 paralogs in the pond-cultured blunt snout bream fish induced by bacterial infection was reported ( 250 ).…”

Section: The Rnase a Superfamilymentioning

confidence: 99%

“…Similarities between unicellular self-defense and mammalian innate immune mechanisms can provide novel strategies to boost our own immune response. For example, macrophage immune regulation by Bacillus RNase (binase) can trigger the host cell anti-tumor response ( 19 ) and the RNaseT2 of the Schistosoma parasite can modulate the host response ( 250 ) and prevent the outbreak of autoimmune diseases or diabetes ( 264 , 265 ). Understanding the uniqueness of RNases and their specificity for cellular RNAs will lead to the development of novel therapeutics.…”

Section: Other Extracellular Rnasesmentioning

confidence: 99%

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Immune Modulation by Human Secreted RNases at the Extracellular Space

et al. 2018

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The ribonuclease A superfamily is a vertebrate-specific family of proteins that encompasses eight functional members in humans. The proteins are secreted by diverse innate immune cells, from blood cells to epithelial cells and their levels in our body fluids correlate with infection and inflammation processes. Recent studies ascribe a prominent role to secretory RNases in the extracellular space. Extracellular RNases endowed with immuno-modulatory and antimicrobial properties can participate in a wide variety of host defense tasks, from performing cellular housekeeping to maintaining body fluid sterility. Their expression and secretion are induced in response to a variety of injury stimuli. The secreted proteins can target damaged cells and facilitate their removal from the focus of infection or inflammation. Following tissue damage, RNases can participate in clearing RNA from cellular debris or work as signaling molecules to regulate the host response and contribute to tissue remodeling and repair. We provide here an overall perspective on the current knowledge of human RNases’ biological properties and their role in health and disease. The review also includes a brief description of other vertebrate family members and unrelated extracellular RNases that share common mechanisms of action. A better knowledge of RNase mechanism of actions and an understanding of their physiological roles should facilitate the development of novel therapeutics.

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Section: Other Extracellular Rnasesmentioning

confidence: 77%

Section: The Rnase a Superfamilymentioning

confidence: 99%

Section: Other Extracellular Rnasesmentioning

confidence: 99%

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Immune Modulation by Human Secreted RNases at the Extracellular Space

et al. 2018

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“…All the data were given in terms of relative mRNA expressed as mean ± SD. Statistical analyses were performed using one-way ANOVA by the least significant difference (LSD) multiple-range test [26], under which differences were considered significant when p < 0.05. Figures were created using GraphPad Prism 7.0 (GraphPad Software, San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene in the Red Swamp Crayfish, Procambarus clarkii

Shi

Han

Fei

et al. 2019

Genes

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The androgenic gland (AG) is a male-specific endocrine organ that controls the primary and secondary sexual characteristics in male crustaceans. More evidence indicates that the insulin-like androgenic gland hormone gene (IAG) is the key male sexual differentiation factor, particularly the application of RNA interference (RNAi) technology on IAG. In this study, the full-length cDNA of IAG (termed PcIAG) was isolated from the red swamp crayfish, Procambarus clarkii. Tissue distribution analysis showed that in addition to its expression in the AG of male P. clarkii, PcIAG was widely expressed in female tissues and other male tissues. The PcIAG protein was detected in the reproductive and nervous systems of adult male P. clarkii. Additionally, RNAi results showed that the PcIAG expression could be silenced efficiently, and the male sperm maturation and release possibly present a transient adverse interference at lower doses (0.1 μg/g and 1 μg/g) of PcIAG–dsRNA (PcIAG double-stranded RNA). Dramatically, the expression level of PcIAG increased sharply shortly after the injection of higher doses (5 μg/g and 10 μg/g) of PcIAG–dsRNA, which might accelerate the maturation and release of sperm. Moreover, the expression of PcSxl (P. clarkii Sex-lethal) was detected by Quantitative Real-Time PCR (qPCR) after the injection of PcIAG–dsRNA to explore whether the PcIAG gene regulates the PcSxl gene, and we found that the PcIAG did not directly regulate PcSxl in P. clarkii. The study could help accelerate the progress of PcIAG functional research and provide a useful reference for the single-sex selective breeding of P. clarkii.

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