Differential expression of six novel peroxidase cDNAs from cell cultures of sweetpotato in response to stress

Here, seven new class III peroxidase genes of Rubia cordifolia L., RcPrx01-RcPrx07, were isolated and characterized. Expression of the Prx genes was studied in R. cordifolia aerial organs as well as in cells transformed with the rolB and rolC genes of Agrobacterium rhizogenes and cells transformed with the wild-type A. rhizogenes A4 strain. In rolC- and rolB-transformed cells, the rol genes were expressed under the control of the 35S promoter, whereas in A. rhizogenes A4-transformed cells the rol genes were expressed under the control of their native promoters. All studied peroxidase genes were greatly upregulated in rolB-overexpressing cells. In contrast, overexpression of the rolC gene and expression of the rol genes under the control of their native promoters had little effect on the abundance of peroxidase transcripts. In accordance with this observation, peroxidase activity was substantially increased in rolB cells and was slightly affected in other transformed cells. Our results indicate that rolB strictly affects the regulation of a set of seven R. cordifolia class III peroxidases.

show abstract

“…2. All of the nodes were well supported and were in agreement with previously published phylogenies (Costa et al 2008;Park et al 2003).…”

Section: Phylogenetic Analysissupporting

confidence: 79%

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

et al. 2012

View full text Add to dashboard Cite

show abstract

“…A study in sweet potato showed that the acidic peroxidase gene swpa4 is not expressed in any tissue of the plant during normal growth, but that it is strongly up-regulated upon wounding of leaves, as well as incubation with high concentrations of H 2 O 2 or NaCl (Park et al 2003). This would mean either that the stress factors wounding and some chemicals follow the same pathway leading to peroxidase activation or that swpa4 promoter is involved in several response pathways.…”

Section: Physical Stressesmentioning

confidence: 99%

Peroxidases have more functions than a Swiss army knife

et al. 2005

View full text Add to dashboard Cite

Plant peroxidases (class III peroxidases) are present in all land plants. They are members of a large multigenic family. Probably due to this high number of isoforms, and to a very heterogeneous regulation of their expression, plant peroxidases are involved in a broad range of physiological processes all along the plant life cycle. Due to two possible catalytic cycles, peroxidative and hydroxylic, peroxidases can generate reactive oxygen species (ROS) ( • OH, HOO• ), polymerise cell wall compounds, and regulate H 2 O 2 levels. By modulating their activity and expression following internal and external stimuli, peroxidases are prevalent at every stage of plant growth, including the demands that the plant meets in stressful conditions. These multifunctional enzymes can build a rigid wall or produce ROS to make it more flexible; they can prevent biological and chemical attacks by raising physical barriers or by counterattacking with a large production of ROS; they can be involved in a more peaceful symbiosis. They are finally present from the first hours of a plant's life until its last moments. Although some functions look paradoxical, the whole process is probably regulated by a fine-tuning that has yet to be elucidated. This review will discuss the factors that can influence this delicate balance.Keywords Evolution . ROS . (abiotic and biotic) stress . Cell wall loosening and cross-linking . Senescence . Fruit ripening . Symbiosis Multigenic family, evolution and homologyHeme peroxidases specific to plants belong to a superfamily that contains three different classes of peroxidases Communicated by P. Kumar

show abstract

“…MV is a well-known ROS generating chemical which causes a massive, light-mediated accumulation of superoxide radicals in plant tissues (19). Six POD genes from the suspension cultured cells showed increased expression by MV treatment (13). In the rice plant, certain POD genes expressed in roots were also induced by MV-mediated oxidative stress (20).…”

Section: Differential Expression Of Three Pod Genes In Response To a mentioning

confidence: 99%

“…Two primers (5'-CACTTCCACGACTGCTTCGT-3', 5'-ACGA-AGCAGTCGTGGAAGTG-3') designed from the conserved regions of previously reported POD sequences were employed (8,10,13).…”

Section: Preparation Of Pod Probesmentioning

confidence: 99%

“…Ten POD cDNAs were isolated from sweetpotato cell cultures, and their expression levels were characterized to understand functions of each POD in response to a variety of environmental stresses, including wounding, chilling, exposure to stress-related chemicals, air pollutants and pathogenic infection (8)(9)(10)(11)(12)(13). In this study, in order to further study functions of POD genes in sweetpotato, we isolated three additional POD genes from the dehydrated fibrous roots of sweetpotato and analyzed expression profiles of each POD gene under various stress conditions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cloning of peroxidase cDNAs from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress

Kim

Yang²,

Kim³

et al. 2008

BMB Reports

View full text Add to dashboard Cite

Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of the pI values of mature proteins. Fluorescence microscope analysis indicated that three PODs are secreted into the extracellular space. RT-PCR analysis revealed that POD genes have diverse expression patterns in a variety of plant tissues. Swpb4 was abundantly expressed in stem tissues, whereas the expression levels of swpb5 and swpa7 transcripts were high in fibrous and thick pigmented roots. Swpb4 and swpa7 showed abundant expression levels in suspension cultured cells. Three POD genes responded differently in the leaf and fibrous roots in response to a variety of stresses including dehydration, temperature stress, stress-associated chemicals, and pathogenic bacteria.

show abstract

Differential expression of six novel peroxidase cDNAs from cell cultures of sweetpotato in response to stress

Cited by 40 publications

References 38 publications

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

Peroxidases have more functions than a Swiss army knife

Molecular cloning of peroxidase cDNAs from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress

Contact Info

Product

Resources

About