Differential expression of proteins in maize roots in response to abscisic acid and drought

Abstract

Roots are highly sensitive organ in plant response to drought, which commonly inhibits root growth. However, less is known about the effect of ABA on root protein expression induced by drought. To help clarify the role of ABA in protein expression of root response to drought, root protein patterns were monitored using a proteomic approach in maize ABA-deficient mutant vp5 and its wild-type Vp5 exposed to drought. Two-dimensional electrophoresis was used to identify droughtresponsive protein spots in maize roots. After coomassie brilliant blue staining, approximately 450 protein spots were reproducibly detected on each gel, wherein 22 protein spots related to ABA or drought were identified using MALDI-TOF MS. Results showed that the 22 proteins are involved in such several cellular processes as energy and metabolism, redox homeostasis and regulatory. An anionic peroxidase and two putative uncharacterized proteins were up-regulated by drought in ABA-dependent way; A glycine-rich RNA binding protein 2, pathogenesis-related protein 10, an enolase, a serine/threonine-protein kinase receptor and a cytosolic ascorbate peroxidase were up-regulated by drought in both ABA-dependent and ABAindependent way; a nuclear transport factor 2, a nucleoside diphosphate kinase, a putative uncharacterized protein and a peroxiredoxin-5 were up-regulated by drought in ABAindependent way; a superoxide dismutase 4A, a VAP27-2, a transcription factor BTF3, a glutathione S-transferase GSTF2 and a putative uncharacterized protein were up-regulated by drought in ABA-dependent way, but not exogenous ABA treatment in the absence of drought; a O-methyltransferase and a putative uncharacterized proteins were down-regulated by ABA and drought. The identification of some novel proteins in the drought response provides new insights that can lead to a better Communicated by Z

Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

“…The possibility that ABA might actually play a role in the control of gene expression upon rehydration is also suggested by the increase in abundance of transcripts encoding several TFs that are often targets for ABA signaling pathways. The zinc finger A20 and AN1 domain-containing stress-associated protein is reportedly induced by drought and ABA [95], as are the NAC type BTF3 transcription factors [96]. It is also noteworthy that transcripts for a two-component sensor histidine kinase accumulate during the rehydration process, as these proteins are often associated with plant responses to environmental changes in and have been closely linked to redox sensing under abiotic stress [97].…”

Recent advances in molecular tool development for drought tolerance breeding in cereal crops: a review

“…They found an increase in quantity of these proteins in leaves of plants subjected to water stress. Hu et al (2011) found a differential expression of 22 proteins in maize roots in response to drought stress.…”

“Omics” of Maize Stress Response for Sustainable Food Production: Opportunities and Challenges

“…The ABA pathway is one of the primary signaling pathways that mediate maize adaptation to drought stress (Bahrun et al, 2002). To further understand the mechanism by which ABA regulates the maize proteome in response to drought, we analyzed the proteomic differences between the ABA-deficient maize mutant vp5 and the wild-type Vp5 under drought stress, by using 2-DE and MS/MS (Hu et al, 2011;. In maize roots, proteins associated with drought stress were primarily involved in energy and metabolism, redox homeostasis, and regulatory processes (Hu et al, 2011); in maize leaves, the identified proteins were primarily involved in processes such as ATP synthesis, protein synthesis, chlorophyll synthesis, CO 2 fixation, gluconeogenesis, antioxidant defense, and signal transduction.…”

“…To further understand the mechanism by which ABA regulates the maize proteome in response to drought, we analyzed the proteomic differences between the ABA-deficient maize mutant vp5 and the wild-type Vp5 under drought stress, by using 2-DE and MS/MS (Hu et al, 2011;. In maize roots, proteins associated with drought stress were primarily involved in energy and metabolism, redox homeostasis, and regulatory processes (Hu et al, 2011); in maize leaves, the identified proteins were primarily involved in processes such as ATP synthesis, protein synthesis, chlorophyll synthesis, CO 2 fixation, gluconeogenesis, antioxidant defense, and signal transduction. Most of the proteins that differentially accumulated in leaves were localized to the chloroplasts and functioned in an ABA-dependent manner in response to drought and light stress (Hu et al, 2012).…”

“…As determined by omics studies, the proteins involved in the maize drought response primarily include protective proteins (such as HSPs) (Benesova et al, 2012;Hu et al, 2010a;Li et al, 2009;Luo et al, 2010), late embryogenesisabundant proteins (LEAs) (Benesova et al, 2012;Huang et al, 2012), stress response-related proteins (such as NBS-LRR resistance-like protein) (Hu et al, 2012), 14-3-3-like proteins (Huang et al, 2012;Li et al, 2009), phytohormonerelated proteins, and signaling proteins (such as auxinrepressed protein and serine/threonine protein kinase) (Bonhomme et al, 2012;Hu et al, 2011;Luo et al, 2010).…”