Differential expression of porcine sperm microRNAs and their association with sperm morphology and motility

Curry, Erin; Safranski, Timothy J.; Pratt, Scott L.

doi:10.1016/j.theriogenology.2011.06.025

Cited by 107 publications

(73 citation statements)

References 44 publications

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“…Our data indicates that over 100 miRNAs can be found within human spermatozoa, a number much more in agreement with other studies in murine and porcine spermatozoa, and in humans comparing the seminal plasma miRNA profiles of normozoospermic men with those suffering from asthenozoospermia and azoospermia. 40,46,47 Of the 130 miRNAs identified here, 28 were significantly (p < 0.05, after BH correction) differentially expressed in smokers compared with non-smokers ( Table 2 and Fig. 4).…”

Section: Discussionmentioning

confidence: 87%

Smoking induces differential miRNA expression in human spermatozoa: A potential transgenerational epigenetic concern?

et al. 2012

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Section: Discussionmentioning

confidence: 87%

Smoking induces differential miRNA expression in human spermatozoa: A potential transgenerational epigenetic concern?

et al. 2012

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“…In the mature testes, 51 miRNAs are upregulated and 78 are downregulated, suggesting that a large number of miRNAs participate in spermatogenesis . The expression levels of let-7a, let-7d, let-7e and miR-22 were higher whereas that of miR-15b lower in abnormal sperm compared with normal sperm (Curry et al, 2011); thus, these miRNAs might be involved in sperm development. MiR-122 is an important regulator of lipid metabolism.…”

Section: Mirnas In Pigsmentioning

confidence: 93%

MicroRNAs in farm animals

Wang

Jiang

2013

Animal

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MicroRNAs (miRNAs) are a class of ,22 nucleotide-long small noncoding RNAs that target mRNAs for translational repression or degradation. miRNAs target mRNAs by base-pairing with the 3 0 -untranslated regions (3 0 -UTRs) of mRNAs. miRNAs are present in various species, from animals to plants. In this review, we summarize the identification, expression, and function of miRNAs in four important farm animal species: cattle, chicken, pig and sheep. In each of these species, hundreds of miRNAs have been identified through homology search, small RNA cloning and next generation sequencing. Real-time RT-PCR and microarray experiments reveal that many miRNAs are expressed in a tissue-specific or spatiotemporal-specific manner in farm animals. Limited functional studies suggest that miRNAs have important roles in muscle development and hypertrophy, adipose tissue growth, oocyte maturation and early embryonic development in farm animals. Increasing evidence suggests that single-nucleotide polymorphisms in miRNA target sites or miRNA gene promoters may contribute to variation in production or health traits in farm animals.

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“…The miRNA family is the best Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796] www.reproduction-online.org characterized of the small RNA species in sperm. They were first identified in humans (Ostermeier et al 2005), followed by confirmation of their presence in mice (Amanai et al 2006) and have now been detected in various mammals, including porcine (Curry et al 2011, Kasimanickam & Kastelic 2016, bulls (Stowe et al 2014), stallions (Das et al 2013), rats and rabbits . It has been demonstrated that mature miRNAs that are carried into the zygote by sperm during the fertilization are relatively rare compared with oocyte miRNAs (Yang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

show abstract

Differential expression of porcine sperm microRNAs and their association with sperm morphology and motility

Cited by 107 publications

References 44 publications

Smoking induces differential miRNA expression in human spermatozoa: A potential transgenerational epigenetic concern?

Smoking induces differential miRNA expression in human spermatozoa: A potential transgenerational epigenetic concern?

MicroRNAs in farm animals

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

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