Differential expression of P450 aromatase during gonadal sex differentiation and sex reversal of the newt Pleurodeles waltl

Kuntz, Sandra; Chesnel, Amand; Duterque-Coquillaud, Martine; Grillier-Vuissoz, Isabelle; M, Callier; Dournon, Christian; Flament, Stéphane; Chardard, Dominique

doi:10.1016/s0960-0760(03)00009-8

Cited by 48 publications

(54 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, it was reported that in vitro treatment of indifferent gonads by the aromatase inhibitor made male phenotype in Xenopus laevis (Miyata and Kubo 2000). In P. waltl, the aromatase activity was suppressed in gonads of ZW larvae reared at male-inducing, high temperatures (Chardard et al 1995;Kuntz et al 2003).…”

Section: Methodsmentioning

confidence: 99%

“…It was reported to be involved in not only normal development of ovary, but also temperature-dependent sex reversal (Chardard et al 1995;Chardard and Dournon, 1999: Kuntz et al 2003 in urodeles, TSD in many reptiles (Desvages et al 1993;Murdock and Wibbels 2003a), and natural sex changes (Liu et al 2004) and temperature-sensitive sex reversal (Kitano et al 1999) in fishes. We have shown in H. retardatus that sexual dimorphism in the P450arom expression was observed as early as 15 days after hatching when the morphological differentiation of gonads had not been occurred (Sakata et al 2005).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander

2006

View full text Add to dashboard Cite

When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP: from 15 to 30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 cDNAs were isolated and their expression pattern was analyzed by competitive and conventional RT-PCR. While P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, strong expression of the P450arom and complete suppression of the Dmrt-1 genes were induced in all experimental larvae. Up-regulation of the P450arom and down-regulation of Dmrt-1 genes even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander

2006

View full text Add to dashboard Cite

show abstract

“…Indeed, at most of the larval stages, gonads alone could not be dissected out due to their small size. In situ hybridization analyses have shown the absence of aromatase expression in the mesonephros (Kuntz et al 2003b). …”

Section: Animalsmentioning

confidence: 99%

“…The detailed protocol for reverse transcription has been described previously (Kuntz et al 2003b). Total RNA was extracted from the different tissues using TRIzol reagent (Invitrogen) and quantified.…”

Section: Rt-pcr Analysesmentioning

confidence: 99%

“…For instance, estradiol is able to counteract the masculinizing effect of temperature (Zaborski 1986) and besides, the increase in aromatase activity that occurs in control ZW larvae from stage 52 is not observed in heat-treated ZW larvae (Chardard et al 1995). In order to determine if this could be the result of different transcription levels, we have recently cloned the gene encoding P. waltl P450-aromatase from adult gonads (Kuntz et al 2003b). We have found a twofold decrease in aromatase expression in heat-treated versus control ZW larvae at stage 54 (Kuntz et al 2003b), suggesting that posttranslational events are also probably able to explain the high difference (40-fold) in enzymatic activity that is usually observed at this stage (Chardard et al 1995).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cerebral and gonadal aromatase expressions are differently affected during sex differentiation of Pleurodeles waltl

Kuntz¹,

Chesnel²,

Flament³

et al. 2004

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

In vertebrates, sex is determined essentially by two means, genetic factors located on sex chromosomes and epigenetic factors such as temperature experienced by the individual during development. Steroids, especially estrogens, are clearly involved in gonadal differentiation in non-mammalian vertebrates. In this regard, the expression of the estrogen-producing enzyme, aromatase, has been shown to be temperature-sensitive in species where temperature can reverse sex differentiation, especially in our model, the amphibian Pleurodeles waltl. We investigated here the regulation of aromatase expression in the brain during sex differentiation in Pleurodeles. We first isolated a brain isoform of aromatase mRNA which differs in its 58 untranslated region from the isoform previously isolated from adult gonads. In adult Pleurodeles, the brain isoform is mainly expressed in brain tissue while the other isoform is gonad specific. Thus, regulation of aromatase expression in P. waltl could occur by alternative splicing of non-coding exon 1 as previously described in mammals. We then investigated aromatase expression in the brain of male and female larvae and found no differences with regard to sex. Measures of aromatase activity in the brain also showed no differences between sexes at larval stages whereas activity markedly increases in the ovary concomitant with the start of gonadal differentiation. These results support the hypothesis that aromatase could be a target of a temperature-sensitive sex-reversing effect in the gonads but not in the brain.

show abstract

Evidence for a conserved role of retinoic acid in urodele amphibian meiosis onset

Wallacides¹,

Chesnel²,

Chardard³

et al. 2009

Developmental Dynamics

View full text Add to dashboard Cite

Pleurodeles waltl is a urodele amphibian displaying a ZZ/ZW genetic mode of sex determination. Gonad differentiation can later be modulated by hormone treatment. To investigate germ cell differentiation, we analyzed the expression of the meiosis marker PwDmc1 and show that germ cells enter meiosis in late larval life in females, and 2 months after metamorphosis in males. Organotypic cultures of gonad-mesonephros complexes demonstrated that retinoic acid triggers meiosis entry in P. waltl. In vivo analyses of both PwRaldh2 and PwCyp26b1 expressions, the enzymes required for RA synthesis and degradation respectively, indicate that meiosis onset depends on PwCyp26b1 repression in the gonad during normal or steroid-induced sex-reversed development. Taken together, our results show that RA-dependent meiosis entry could be a conserved mechanism of germ cell differentiation in vertebrates and provide evidence for crosstalk between steroid and RA signaling in the course of sex differentiation. Developmental Dynamics 238:1389 -1397, 2009.

show abstract

Differential expression of P450 aromatase during gonadal sex differentiation and sex reversal of the newt Pleurodeles waltl

Cited by 48 publications

References 41 publications

Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander

Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander

Cerebral and gonadal aromatase expressions are differently affected during sex differentiation of Pleurodeles waltl

Evidence for a conserved role of retinoic acid in urodele amphibian meiosis onset

Contact Info

Product

Resources

About