Differential Expression of Ovarian Cathepsins B, D and L during Oocyte Growth in Scad

Aranishi, Futoshi

doi:10.1262/jrd.46.287

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…For pelagophil teleosts, Catb has also been shown to be a major protease in barfin flounder ( Verasper moseri ) (Matsubara et al . 2003) and scad ( Decapturus maruadsi ) (Aranishi 2000a). In this latter species, coexistent Catd is suggested to activate the Catb zymogen (Aranishi 2000b).…”

Section: Lazy Lysosomes Release Faa From Yolk Proteinsmentioning

confidence: 99%

Requirement for amino acids in ontogeny of fish

Finn

Fyhn

2010

Aquaculture Research

147

114

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Amino acids are vital for all living organisms. During early fish ontogeny, they are important fuel molecules, signalling factors and major substrates for the synthesis of a wide range of bioactive molecules and proteins. Because the majority of fish eggs are cleidoic, i.e. closed free‐living systems following ovulation and activation, early development of fish depends on the maternal provision of amino acids during oogenesis. While more than 600 proteins have been identified in the growing oocytes of fish, the major vehicles for supplying amino acids to the growing oocyte before ovulation are the vitellogenins, of which many genes and multiple forms are known. Here we review the importance of amino acids for the intermediary metabolism of fish embryos and larvae, where amino acids have been shown to be the preferred catabolic substrate. Subsequently, we address the specialization of the lysosomal pathway involved in the uptake and degradation of yolk proteins. This latter pathway is specifically modified in the germline to facilitate the long‐term storage of egg yolk proteins. In marine teleosts, the degradative pathway may be activated before fertilization during oocyte maturation to release free amino acids for oocyte hydration and the acquisition of egg buoyancy. In other species, including freshwater fish, a more latent activation of acid hydrolases occurs after fertilization during the four phases of yolk resorption. The developmental contributions of the yolk syncytial layer, vitelline circulation and liver are essential components of the amino acid supply during fish ontogeny.

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Section: Lazy Lysosomes Release Faa From Yolk Proteinsmentioning

confidence: 99%

Requirement for amino acids in ontogeny of fish

Finn

Fyhn

2010

Aquaculture Research

147

114

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“…Teleost Ctsl has been shown to play crucial roles in the formation and/or degradation of yolk during early and late oocyte growth [3,[41][42][43][44]. More specifically, it has been shown to contribute to the processing and proteolysis of vitellogenin in the yolk.…”

Section: Discussionmentioning

confidence: 99%

Involvement of cathepsin L in the degradation and degeneration of postovulatory follicle of the medaka ovary

Kondo,

Rajapakse,

Ogiwara

2023

Biology of Reproduction

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Cathepsin L (CTSL) plays physiological and pathological roles in immune responses, cancer, metamorphosis, and oogenesis in several species. However, the function of Ctsl in medaka ovaries remains unclear. Therefore, here, we examined the physiological functions of Ctsl in the medaka ovaries. Ctsl mRNA transcripts and proteins were found to be constitutively expressed in the ovaries of Oryzias latipes over a 24-h spawning cycle. Expression was localized within the oocyte cytoplasm of growing follicles and the follicle layer of preovulatory and postovulatory follicles. Moreover, the active form of Ctsl was highly expressed in the follicle layer of periovulatory follicles and the ovaries 2–6 h after ovulation. Recombinant Ctsl (rCtsl) was activated under acidic conditions and exhibited enzymatic activity in acidic and neutral pH conditions. However, extracellular matrix (ECM) proteins were degraded by rCtsl under acidic, not neutral pH conditions. Ctsl was secreted from preovulatory follicles, while active rCtsl was detected in the conditioned medium of a medaka cell line, OLHNI-2. Mechanistically, rCtsl activates recombinant urokinase-type plasminogen activator-1 (Plau1), which is expressed within the follicle layers post-ovulation. Meanwhile, the treatment of medakas with an E-64 or anti-Ctsl antibody effectively blocked follicular layer degeneration and degradation after ovulation, whereas in vitro ovulation was not inhibited by either. Collectively, the findings of this study indicate that although Ctsl does not impact ovulation in medakas, it contributes to the degeneration and degradation of the follicle layers following ovulation via activation of Plau1, and not via the degradation of ECM proteins.

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“…CatB is thought to participate in the embryonic development of juvenile fish. During the oocyte growth period of scad ( Decapterus maruadsi ), for example, high‐level expression of CatB activity characterizes one of the main proteases involved in hydrolyzing yolk protein; the extent of hydrolysis influences the seasonal yield of fish eggs and quality of juvenile fish (Aranishi ). To this effect, observing the expression of CatB during the development of juvenile fish may be significant for evaluating the yield and quality of fish—this is of considerable importance for commercial fishes, as well.…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of Jian Carp (Cyprinus Carpio Var. Jian) Cathepsin B: Cloning, Expression, Characterization, and Antibody Preparation

Zhong

et al. 2017

Journal of Food Science

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Cathepsin B (CatB) cDNA of 759 bp from Jian carp (Cyprinus carpio var. Jian) with amino acid similarity of 99.6% to common carp was cloned. The mature CatB was expressed in Escherichia coli BL21 transferred with vector CatB-pET-30a. It was purified and identified as a single band (29 kDa) on SDS-PAGE. Optimum CatB activity was observed at 40 °C and pH 5.5. Mouse anti-CatB polyclonal antibody with a high titer of 1:256000 was prepared successfully and shown to specifically recognize the antigen both in prokaryotic cells and in the tissues of Jian carp according to western blotting and immunohistochemistry results. Immunolocation analysis showed that CatB distribution at protein level varied among the tested tissues. The results presented in this study may provide a significant reference for future research on the inherent relationship between CatB and the quality of fish or fish products at both the gene and protein levels.

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Differential Expression of Ovarian Cathepsins B, D and L during Oocyte Growth in Scad

Cited by 3 publications

References 23 publications

Requirement for amino acids in ontogeny of fish

Requirement for amino acids in ontogeny of fish

Involvement of cathepsin L in the degradation and degeneration of postovulatory follicle of the medaka ovary

Immunolocalization of Jian Carp (Cyprinus Carpio Var. Jian) Cathepsin B: Cloning, Expression, Characterization, and Antibody Preparation

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