Differential expression of osteoblast and osteoclast chemmoatractants in compression and tension sides during orthodontic movement

Garlet, Thiago; Coelho, Ulisses; Repeke, Carlos; Silva, João; Cunha, Fernando; Garlet, Gustavo

doi:10.1016/j.cyto.2008.03.003

Cited by 114 publications

(80 citation statements)

References 43 publications

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“…22,23 Other groups have noted the in vivo expression of RANKL by PDL cells through immunohistochemistry and RT-PCR in the compression side after one day of orthodontic force, but did not look at earlier time points. 5,14 In our model, we see RANKL expression after only 3 hours of force. After 24 hours, however, the ligand is no longer expressed, likely due to interaction with RANK or release of the ligand into the extracellular matrix.…”

Section: The Rat Otm Model Accurately Replicates the Cellular And Molmentioning

confidence: 99%

“…1,4 Stresses generated on the tension side result in the differentiation of mesenchymal cells into osteoblasts. 1,5 Communication occurs between the osteoblast and osteoclast populations, eg, osteoblasts and their precursors aid in the differentiation of osteoclasts through expression of tumor necrosis factor ligand superfamily member 11, or receptor activator of nuclear factor kappa ␤ ligand (RANKL), a transmembrane or soluble protein also found on fibroblasts, stromal cells, and T-cells. [6][7][8] Interaction between receptor activator of nuclear factor kappa ␤ (RANK) on the preosteoclasts and RANKL promotes maturation and fusion of the preosteoclasts into activated osteoclasts.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Markers of Early Orthodontic Tooth Movement

Brooks

Nilforoushan

Manolson

et al. 2009

The Angle Orthodontist

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Objective: To understand the molecular basis of early orthodontic tooth movement by looking at the expression of KI-67, runt-related transcription factor 2 (Runx2), and tumor necrosis factor ligand superfamily member 11 (RANKL) proteins. Materials and Methods:We employed a rat model of early orthodontic tooth movement using a split-mouth design (where contralateral side serves as a control) and performed immunohistochemical staining to map the spatial expression patterns of three proteins at 3 and 24 hours after appliance insertion. Results: We observed increased expression of KI-67, a proliferation marker, and RANKL, a molecule associated with osteoclastic differentiation, in the compression sites of the periodontal ligament subjected to 3 hours of force. In contrast, there was increased expression of KI-67 and Runx2, a marker of osteoblast precursors, in tension areas after 24 hours of force. Decreased KI-67 expression in the mesial and distal regions of the periodontal ligament was observed at the midpoint of the tooth root. Conclusions:The early RANKL expression indicates that at this early stage cells are involved in osteoclast precursor signaling. Also, decreased KI-67 expression found near the midpoint of the tooth root is believed to represent the center of rotation, providing a molecular means of visualizing mechanical loading patterns.

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Section: The Rat Otm Model Accurately Replicates the Cellular And Molmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Markers of Early Orthodontic Tooth Movement

Brooks

Nilforoushan

Manolson

et al. 2009

The Angle Orthodontist

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“…Along with this process, cytokines, growth factors, transformation factors, and neurotransmitters are released (Bollen et al 2008), creating a proper environment for bone remodeling. In this environment, bone resorption is observed on the compression side of the PDL fibers, while bone formation is observed on the tension side (Garlet et al 2008).…”

Section: Introductionmentioning

confidence: 99%

The influence of non-steroidal anti-inflammatory drugs and paracetamol used for pain control of orthodontic tooth movement: a systematic review

Corrêa

Almeida

Lopes

et al. 2017

An. Acad. Bras. Ciênc.

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The present study aimed to perform a systematic literature review to determine if there is a non-steroidal anti-inflammatory drug (NSAID) that interferes less within tooth movement. This research was performed according to the PRISMA statement. Articles were searched in eight electronic databases (PubMed, Scopus, Embase, Web of Science, LILACS, SciELO, Google Scholar, and Open Grey). Only experimental studies on male Wistar rats were selected, which included experiments related to the influence of NSAIDs on orthodontic movement. Studies in animals with pathological conditions, literature review articles, letters to the editor and/or editorials, case reports, abstracts, books, and book chapters were excluded. Each of the steps of this systematic literature review was performed by two examiners independently. Results: the total sample consisted of 505 articles, from which 6 studies were eligible after a qualitative analysis. From the drugs assessed, paracetamol was unanimous for not interfering within orthodontic movement when compared to the control group. However, drugs such as aspirin, ibuprofen, sodium diclofenac, and selective cyclooxygenase-2 inhibitors caused a reduction in tooth movement when compared to the control group. Conclusion: paracetamol could be considered the drug of choice for pain relief because it interferes less within tooth movement.

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“…Estudos recentes apontam que as citocinas e as quimiocinas são a chave para a compreensão da reabsorção óssea, durante o movimento ortodôntico (Garlet et al, 2008;Andrade et al, 2009;García-López et al, 2013;Kitaura et al, 2014), e para o estudo de condições patológicas inflamatórias, como a gengivite e periodontite (Cochran, 2008;Ramseier et al, 2009). Estes mediadores podem levar à reabsorção óssea e/ou radicular (Consolaro e Consolaro, 2009;Viecilli et al, 2009;de Rossi et al, 2010;De Oliveira et al, 2014).…”

Section: Introductionunclassified

“…Em condições patológicas, como no caso das periodontites, há liberação de citocinas e quimiocinas (Garlet et al, 2008;Andrade et al, 2009;Ramseier et al, 2009;Belibasakis e Bostanci, 2012), pois os tecidos de sustentação reagem às bactérias, aos seus produtos e subprodutos, com uma resposta imunológica e inflamatória (Azuma, 2006;Armitage e Cullinan, 2010;Armitage e Robertson, 2012;), promovendo reabsorção dos tecidos mineralizados (Consolaro e Consolaro, 2009;Viecilli et al, 2009;de Rossi et al, 2010). Além disso, estas proteínas também regulam a reabsorção óssea durante o movimento ortodôntico (Garlet et al, 2008;Andrade et al, 2009). …”

Section: Introductionunclassified

Bráquetes convencionais e autoligados: detecção de micro-organismos na saliva e in situ, avaliação de parâmetros periodontais e quantificação de citocinas no fluido crevicular

Bergamo¹

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Alterações no volume do fluido crevicular e nos parâmetros periodontais, após a colagem de bráquetes metálicos autoligados e convencionais. CAPÍTULO 2 41Micro-organismos relacionados direta ou indiretamente à cárie dental, na saliva e in situ, em diferentes tipos de bráquetes (convencionais e autoligados). CAPÍTULO 3 55Detecção de complexos microbianos pela técnica checkerboard DNA DNA hybridization, pré e pós-colagem de bráquetes convencionais e autoligados. CAPÍTULO 4 75Níveis de citocinas no fluido crevicular e perfil microbiano in situ, após a colagem de bráquetes convencionais e autoligados. ® R e SmartClip TM em incisivos e caninos superiores. Os índices, o volume do fluido e as amostra de saliva (S0) foram obtidos antes da instalação dos aparelhos (T0) e após 30(T1) e 60(T2) dias. Um bráquete de cada tipo foi removido 30 e 60 dias após a colagem. Os dados foram submetidos à análise estatística utilizando-se os testes não-paramétricos de Friedman, Mann-Whitney e Wilcoxon e coeficiente de correlação não-paramétrico de Spearman. A análise da concentração de citocinas no fluido crevicular foi realizada segundo modelo de análise de variância misto, seguida pelo teste de Tukey, com nível de significância de 5%. Não houve correlação entre o grau de apinhamento, overjet e overbite com os escores do índice de placa (PI), índice gengival, índice de sangramento gengival e com o volume do fluido crevicular no tempo inicial T0 (p>0,05). Verificou-se a diferença significativa nos escores de PI e volume do fluido crevicular somente nos dentes que receberam bráquetes autoligados SmartClip TM , entre os tempos T0-T2(p<0,05). Identificou-se a presença de 21 das 22 espécies bacterianas e 4 das 5 espécies fúngicas, na saliva, antes da colagem dos aparelhos ortodônticos. In situ, no bráquete autoligado SmartClip TM observou-se maiores níveis de espécies do complexo vermelho 60 dias após a colagem. Para os bráquetes convencionais Gemini TM verificou-se diminuição nos níveis de espécies do complexo roxo. Para o bráquete autoligado In-Ovation ® R identificou-se diferença significativa para C. rectus 30 dias após a colagem e para S. mutans 60 dias após a colagem. Quando comparou-se os níveis salivares e os in situ, verificou-se maiores níveis de espécies nos bráquetes, in situ, sendo que os autoligados apresentaram os maiores valores.Aumento significante foi verificado para os bráquetes autoligados In-Ovation ® R, para S. mutans e L. casei. Após 60 dias da colagem do aparelho ortodôntico observou-se um aumento nos níveis de TNF-α (p<0.005), em todos os tipos de bráquetes. ABSTRACTThe orthodontic appliance promoting several changes in oral cavity according to the variety of solid and elastic materials used in the treatment, leading to the biofilm development. The aim of this study, in vivo, was: 1) Evaluate the periodontal index: Plaque Index (PI), Gingival Index (GI), Gingival Bleeding Index (GBI)and gingival crevicular fluid volume.2) The levels of cariogenic and periodontopatogenical bacteria in saliva and in two different met...

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Differential expression of osteoblast and osteoclast chemmoatractants in compression and tension sides during orthodontic movement

Cited by 114 publications

References 43 publications

Molecular Markers of Early Orthodontic Tooth Movement

Molecular Markers of Early Orthodontic Tooth Movement

The influence of non-steroidal anti-inflammatory drugs and paracetamol used for pain control of orthodontic tooth movement: a systematic review

Bráquetes convencionais e autoligados: detecção de micro-organismos na saliva e in situ, avaliação de parâmetros periodontais e quantificação de citocinas no fluido crevicular

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