Differential expression of oestrogen receptor and oestrogen inducible genes in gastric mucosa and cancer.

Singh, Sukhdev; Poulsom, Richard; Wright, N A; Sheppard, M. C.; Langman, M. J. S.

doi:10.1136/gut.40.4.516

“…Our findings support those of Wu et al (1992a), finding no significant difference in ER or PR expression in gastric cancer compared with normal gastric mucosa using DCC and EIA assays. This is in contrast to the data reported by Kojima et al (1991) which demonstrated expression of ER in gastric cancer, but not in normal mucosa when measured by immunohistochemistry and recent data from Singh et al (1997) showing decreased expression of ER in gastric cancer compared to normal mucosa. Our data showed no significant difference between receptor-positive and receptor-negative groups on comparison with sex, tumour grade and tumour stage.…”

Section: Discussioncontrasting

confidence: 56%

Expression of oestrogen and progesterone receptors in gastric cancer: a flow cytometric study

Karat

¹

,

Brotherick

²

,

Shenton

³

et al. 1999

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Summary Increased expression of oestrogen (ER) and progesterone (PR) receptors have been reported in gastric adenocarcinoma, although results have been variable. Immunohistochemical staining methodologies, in particular in the detection of ER, have been inconsistent with many tumours being classified ER-negative. In this study we have used flow cytometry to quantify expression of ER and PR in gastric adenocarcinoma and examine their relationships with established prognostic indicators. Cytokeratin-positive cells obtained from tumour biopsies of 50 patients with gastric cancer and ten control patients were labelled with biotinylated ER or PR antibodies followed by streptavidin PE. Flow cytometry was seen to increase the detection of ER levels in gastric cancer with more receptor-positive patients in this study than in results published to date. We believe this is related to the sensitivity of the flow cytometric assay with the detection of small shifts in ER level detected using cytokeratin gating. On analysis, the data showed no significant correlations with tumour stage and grade, and no differences were seen between normal mucosa and gastric cancer samples.

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“…Our findings support those of , finding no significant difference in ER or PR expression in gastric cancer compared with normal gastric mucosa using DCC and EIA assays. This is in contrast to the data reported by Kojima et al (1991) which demonstrated expression of ER in gastric cancer, but not in normal mucosa when measured by immunohistochemistry and recent data from Singh et al (1997) showing decreased expression of ER in gastric cancer compared to normal mucosa. Our data showed no significant difference between receptor-positive and receptor-negative groups on comparison with sex, tumour grade and tumour stage.…”

Section: contrasting

confidence: 56%

Expression of oestrogen and progesterone receptors in gastric cancer: a flow cytometric study

Karat

¹

,

Brotherick

²

,

Shenton

³

et al. 1999

View full text Add to dashboard Cite

Summary Increased expression of oestrogen (ER) and progesterone (PR) receptors have been reported in gastric adenocarcinoma, although results have been variable. Immunohistochemical staining methodologies, in particular in the detection of ER, have been inconsistent with many tumours being classified ER-negative. In this study we have used flow cytometry to quantify expression of ER and PR in gastric adenocarcinoma and examine their relationships with established prognostic indicators. Cytokeratin-positive cells obtained from tumour biopsies of 50 patients with gastric cancer and ten control patients were labelled with biotinylated ER or PR antibodies followed by streptavidin PE. Flow cytometry was seen to increase the detection of ER levels in gastric cancer with more receptor-positive patients in this study than in results published to date. We believe this is related to the sensitivity of the flow cytometric assay with the detection of small shifts in ER level detected using cytokeratin gating. On analysis, the data showed no significant correlations with tumour stage and grade, and no differences were seen between normal mucosa and gastric cancer samples.

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“…A male preponderance (Furukawa et al, 1982a) and a protective effect of female hormones have been reported for stomach cancer in rat experimental models (Furukawa et al, 1982b). As functional oestrogen receptors alpha (Wu et al, 1990;Singh et al, 1997) and beta (Taylor and Al-Azzawi, 2000;Matsuyama et al, 2002) exist in human stomach mucosa, it has been hypothesised that sex hormones, notably oestrogens, protect women against stomach cancer (Sipponen and Correa, 2002).…”

mentioning

confidence: 99%

Parity and risk of stomach cancer by sub-site: a national Swedish study

Bahmanyar

¹

,

Lambe

²

,

Zendehdel

³

et al. 2008

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We investigated stomach cancer risk by anatomic sub-site in relation to parity, as a marker for higher exposure to sex hormones, in a case -control study, nested within a cohort of 2 406 439 Swedish women born in 1925 or later and followed from 1970 or age 30 until emigration, death, any cancer diagnosis, or through 2004, whichever occurred first. We identified 286 cardia and 2498 noncardia stomach cancer cases with five matched controls for each case. Cross-linkage with the Multi-Generation Register provided information about reproductive history. Using conditional logistic regression models for estimating odds ratios (ORs) and corresponding 95% confidence intervals (CIs), adjusted for education level and occupation, we found no association between any aspect of parity and non-cardia stomach cancer (OR ¼ 1.01, 95% CI 0.89 -1.15, comparing parous with nulliparous women). However, a 30% risk reduction for postmenopausal cardia cancer (OR ¼ 0.7, 95% CI 0.4 -1.0) was noted among parous relative to nulliparous women and the risk for premenopausal cardia cancer fell with increasing number of children (P for trend ¼ 0.04). Our results indicate that exposure to female sex hormones does not protect against non-cardia stomach cancer and does not explain male predominance. The observed moderate inverse relationship between parity and cardia cancer may be mediated by nonhormonal factors and warrants further study.

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Differential expression of oestrogen receptor and oestrogen inducible genes in gastric mucosa and cancer.

Abstract: Background-Evidence exists for a role

Cited by 49 publications

References 22 publications

Expression of oestrogen and progesterone receptors in gastric cancer: a flow cytometric study

Expression of oestrogen and progesterone receptors in gastric cancer: a flow cytometric study

Expression of oestrogen and progesterone receptors in gastric cancer: a flow cytometric study

Parity and risk of stomach cancer by sub-site: a national Swedish study

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