Differential expression of nuclear lamin proteins during chicken development.

Abstract

Abstract. By immunocytochemistry, quantitative immunoblotting, and two-dimensional gel electrophoresis, we have analyzed the distribution of nuclear lamin proteins during chicken embryonic development. Whereas no qualitative differences in the patterns of expression of lamins A, B~, and B2 were observed during gametogenesis in either the female or the male germ line, profound changes in the composition of the nuclear lamina occurred during the development of somatic tissues. Most unexpectedly, early chicken embryos were found to contain little if any lamin A, although they contained substantial amounts of lamins B, and B2. During embryonic development, lamin A became increasingly prominent, whereas the amounts of lamin B, decreased in many tissues. Interestingly, the extent and the developmental timing of these changes displayed pronounced tissue-specific variations. Lamin B2 was expressed in fairly constant amounts in all cell types investigated (except for pachytene-stage germ cells). These results have implications for the purported functional specializations of individual lamin proteins. In addition, they suggest that alterations in the composition of the nuclear lamina may be important for the establishment of cell-or tissue-specific differences in nuclear architecture.T He nuclear lamina is a filamentous meshwork closely apposed to the nucleoplasmic surface of the inner nuclear membrane (1; for review see reference 15). It is thought to serve a nucleoskeletal role important for nuclear envelope integrity (8,12,28) and interphase chromatin organization (4,14,17,22); this organization in turn may be important for DNA replication and differential gene expression (for references see 6, 9, 17). Lamin proteins have been found in a wide variety of organisms, including insects, amphibia, birds, and mammals (for review see reference 19). The three major mammalian lamins have been designated as lamins A, B, and C (12). Lamin B is implicated in anchoring the lamina to the nuclear membrane, whereas lamins A and C are thought to interact with chromatin (8,13,15). Based on cDNA sequence data (11, 27) as well as direct structural information obtained from electron microscopy (1), members of the lamin protein family were recently shown to constitute a novel class of (nuclear) intermediate filament proteins.With the aid of monoclonal antibodies we have begun to study the composition and assembly of the nuclear lamina in chicken (24,25). In particular, we have recently characterized three structurally distinct chicken proteins that we have termed lamins A, B~, and B2 (25). In chicken embryo R. Stick's present address is Department of Molecular Biology, University of Geneva, CH-1211 Geneva, Switzerland.fibroblasts lamins A and B2 predominate, whereas lamin B1 represents a quanttafively minor component (25). By immunological criteria, chicken lamin B, is related to mammalian lamin B, whereas chicken lamin B2 is related to lamin A as well as to a quantitatively minor mammalian protein that had not previously been considered as...

Activation of the lamin A gene during rat liver development

“…Studies carried out in several groups have established that the A-type lamin proteins are expressed only in differentiated cells [5][6][7][8]. Hence, the change in composition of the lamina from a predominantly B-type polymer to a heteropolymer of A and B-type lamins during cell differentiation may have important implications.…”

Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association.

“…The mAb 9E10 (ATCC CRL 1729) specifically recognizes an epitope in the decapeptide EQKLISEEDL of the human c-myc protein (Evan et al, 1985), and mAb L3-4B4 (kindly provided by Dr. R. Stick, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany) reacts with -chicken and mammalian lamin A, but not with Xenopus lamins (Lehner et al, 1987;Stick et al, 1988). In one set of experiments, mAb b7-1A9-M9 directed against the histone-binding protein nucleoplasmin from Xenopus …”

S‐Phase phosphorylation of lamin B2

“…This classification is based on amino acid sequence homologies, post-translational modification and behaviour at mitosis [11]. Of these sub-families, B-type lamins appear to be required as functional components of all cells [12,13] whereas A-type lamins are thought to be components of differentiated cells [14].…”