Differential expression of NMDA and AMPA receptor subunits in rat dorsal and ventral hippocampus

Pandis, C.; Sotiriou, Evangelos; Kouvaras, Evangelos; Asprodini, Eftihia; Papatheodoropoulos, Costas; Angelatou, Fevronia

doi:10.1016/j.neuroscience.2006.02.003

Cited by 88 publications

(76 citation statements)

References 62 publications

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“…However, these pharmacological manipulations were not aimed at a particular subregion of the DH (eg, CA1-3, dentate gyrus). NMDARs are mostly postsynaptic in the hippocampus (Nyiri et al, 2003;Takumi et al, 1999), and have a greater expression of NR2B subunits than other NMDA subunits (ie, NR2A) in the CA1 subregion, but not in the CA3 subregion or the dentate gyrus (Coultrap et al, 2005;Pandis et al, 2006). Also, within the CA1 subregion, NMDAR subunit composition and density vary across cell types (Angulo et al, 2004;Nyiri et al, 2003;Serrano et al, 2006;Serrano et al, 2008;Siegel et al, 1994;Szabadits et al, 2011;Todd et al, 2006;Xue et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Role of a Hippocampal Src-Family Kinase-Mediated Glutamatergic Mechanism in Drug Context-Induced Cocaine Seeking

Xie

Arguello

Wells

et al. 2013

Neuropsychopharmacol

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Glutamatergic neurotransmission in the dorsal hippocampus (DH) is necessary for drug context-induced reinstatement of cocaineseeking behavior in an animal model of drug relapse. Furthermore, in vitro studies suggest that the Src family of tyrosine kinases critically regulates glutamatergic cellular functions within the DH. Thus, Src-family kinases in the DH may similarly control contextual cocaineseeking behavior. To test this hypothesis, rats were trained to lever press for un-signaled cocaine infusions in a distinct context followed by extinction training in a different context. Cocaine-seeking behavior (non-reinforced active lever pressing) was then assessed in the previously cocaine-paired and extinction contexts after AP5 (N-methyl-D-aspartate glutamate (NMDA) receptor (NMDAR) antagonist; 0.25 or 2.5 mg/0.5 ml/hemisphere), PP2 (Src-family kinase inhibitor; 6.25 or 62.5 ng/0.5 ml/hemisphere), Ro25-6981 (NR2B subunitcontaining NMDAR antagonist; 0.2 or 2 mg/0.5 ml/hemisphere), or vehicle administration into the DH. Administration of AP5, PP2, or Ro25-6981 into the DH dose-dependently impaired drug context-induced reinstatement of cocaine-seeking behavior relative to vehicle, without altering instrumental behavior in the extinction context or food-reinforced instrumental responding and general motor activity in control experiments. Cocaine-seeking behavior during the first 20 min of the test session in the cocaine-paired context was associated with an increase in NR2B subunit activation, and intra-DH PP2 pretreatment disrupted this relationship. Together, these findings suggest that Src-family kinase activation, NMDAR stimulation, and likely Src-family kinase-mediated NR2B subunit-containing NMDAR activation in the DH are necessary for incentive motivational and/or memory processes that promote contextual cocaine-seeking behavior.

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Section: Discussionmentioning

confidence: 99%

Role of a Hippocampal Src-Family Kinase-Mediated Glutamatergic Mechanism in Drug Context-Induced Cocaine Seeking

Xie

Arguello

Wells

et al. 2013

Neuropsychopharmacol

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“…Results from previous studies have shown that NMDARs differ between the two hippocampal poles regarding their subunit composition and the functionality under conditions of endogenous or exogenous activation. Specifically, the different subunit composition of NMDARs between the DH and the VH (Pandis et al 2006) suggested that NMDARs permit larger currents in VH than that in DH. Furthermore, strong presynaptic activity (Papatheodoropoulos 2015b) or exogenous LTP in dorsal and ventral hippocampus www.learnmem.org activation of NMDARs (Kouvaros and Papatheodoropoulos 2016) produces greater NMDAR-dependent responses in the VH than that in the DH.…”

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confidence: 99%

High-frequency stimulation-induced synaptic potentiation in dorsal and ventral CA1 hippocampal synapses: the involvement of NMDA receptors, mGluR5, and (L-type) voltage-gated calcium channels

Papatheodoropoulos

Kouvaros

2016

Learn. Mem.

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The ability of the ventral hippocampus (VH) for long-lasting long-term potentiation (LTP) and the mechanisms underlying its lower ability for short-lasting LTP compared with the dorsal hippocampus (DH) are unknown. Using recordings of field excitatory postsynaptic potentials (EPSPs) from the CA1 field of adult rat hippocampal slices, we found that 200-Hz stimulation induced nondecremental LTP that was maintained for at least 7 h and was greater in the DH than in the VH. The interaction of NMDA receptors with L-type voltage-dependent calcium channels appeared to be more effective in the DH than in the VH. Furthermore, the LTP was significantly enhanced in the DH only, between 2 and 5 h post-tetanus. Furthermore, the mGluR5 contributed to the post-tetanic potentiation more in the VH than in the DH.The hippocampus is a brain structure with paramount capacity for phenomena of short-term and long-term synaptic plasticity (Bliss et al. 2007). Remarkably, this ability is not uniformly distributed along the long axis of the structure. Namely, the ability for longterm potentiation (LTP) induced by high-frequency stimulation (HFS) is strikingly lower in the ventral hippocampus (VH) compared with the dorsal hippocampus (DH) (Papatheodoropoulos and Kostopoulos 2000;Maruki et al. 2001;Colgin et al. 2004;Maggio and Segal 2009;Kenney and Manahan-Vaughan 2013;Keralapurath et al. 2014). This is one of the most notable differences recently found in the intrinsic circuitry between the DH and the VH. Long-term synaptic plasticity is thought to be a fundamental mechanism that supports learning and memory (Matynia et al. 2002;Takeuchi et al. 2014). Thus, differences in the ability for LTP induction might contribute to the well-known functional segregation along the long (dorsoventral or septotemporal) axis of the hippocampus (Moser and Moser 1998;Fanselow and Dong 2010;Bast 2011;Goosens 2011;Small et al. 2011;Bannerman et al. 2014;Strange et al. 2014). Despite the established difference in the magnitude of HFS-induced LTP between the two hippocampus segments, the underlying mechanisms remain unknown. This is because, although HFS-induced LTP can be blocked by antagonists of NMDARs (Park et al. 2014), HFS might induce compound LTP in the CA1 field with the participation of L-type voltage-dependent calcium channels (L-VDCCs), (Grover and Teyler 1990;Cavus and Teyler 1996;Morgan and Teyler 2001;Bayazitov et al. 2007) and of metabotropic glutamate receptor-5 (mGluR5) (Jia et al. 1998). NMDAR-dependent and L-VDCC-dependent LTP might have distinct implication in certain types of learning and memory (Morris et al. 1986;Tsien et al. 1996;Borroni et al. 2000;Moosmang et al. 2005). In addition, NMDAR-LTP and L-VDCC-LTP might preferentially be involved in retention of information over short and long periods of time, respectively (Borroni et al. 2000).A parameter of LTP particularly relevant for the implications of LTP in the memory function is the persistence of potentiation (Martin et al. 2000;Lynch 2004). Nondecremental LTP lasting for severa...

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“…Although, lower levels of mRNA and protein expression for NR2A, NR2B subunits was reported in CA1 region of VH, compare to CA1 region of DH [10], it was suggested that in the low Mg 2+ model of ictogenesis the contribution of NMDA receptors was greater in VH than in DH in adults rats [5]. This discrepancy could be at least part ly explained by the difference in the ratio of NMDA receptor subunits NR2A and NR2B in VH and DH [10], with NR2A subunit prevailing in the DH. The decreased ratio of NR2A/NR2B in VH compared to DH implies the prolongation of the decay time of the NMDAR-mediated synaptic current and a weaker sensitivity of the NMDA receptor channel to Mg 2+ [14].…”

Section: Resultsmentioning

confidence: 82%

“…Lowering Mg 2+ in an extracellular solution has been shown to create a hyperexcitable environment conducive for seizure generation by decreasing membrane surface charge screening, lowering Mg 2+ -sensitive Ca 2+ channel block, and decreasing the Mg 2+ blockade of N-methyl-D-aspartate (NMDA) receptors [11][12][13]. Although, lower levels of mRNA and protein expression for NR2A, NR2B subunits was reported in CA1 region of VH, compare to CA1 region of DH [10], it was suggested that in the low Mg 2+ model of ictogenesis the contribution of NMDA receptors was greater in VH than in DH in adults rats [5]. This discrepancy could be at least part ly explained by the difference in the ratio of NMDA receptor subunits NR2A and NR2B in VH and DH [10], with NR2A subunit prevailing in the DH.…”

Section: Resultsmentioning

confidence: 99%

“…It was shown that ventral hippocampus (VH) is more vulnerable to the abnormal synchronization and dorsal part of hippocampus (DH) is more resistant to seizureinduced injury and neuronal death than VH [5,6,8,9]. The difference in seizure susceptibility of DH and VH could be at least partly explained by the difference in the receptor expression in different parts of hippocampus [8,10].…”

Section: Introductionmentioning

confidence: 99%

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Effect of altered extracellular magnesium concentration on the neuronal activity in different hippocampal regions of immature rats

Власюк¹,

Romanov²,

Isaev³

et al. 2017

Fiziol Zh

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Differential expression of NMDA and AMPA receptor subunits in rat dorsal and ventral hippocampus

Cited by 88 publications

References 62 publications

Role of a Hippocampal Src-Family Kinase-Mediated Glutamatergic Mechanism in Drug Context-Induced Cocaine Seeking

Role of a Hippocampal Src-Family Kinase-Mediated Glutamatergic Mechanism in Drug Context-Induced Cocaine Seeking

High-frequency stimulation-induced synaptic potentiation in dorsal and ventral CA1 hippocampal synapses: the involvement of NMDA receptors, mGluR5, and (L-type) voltage-gated calcium channels

Effect of altered extracellular magnesium concentration on the neuronal activity in different hippocampal regions of immature rats

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