Differential expression of N-Myc downstream regulated gene 2 (NDRG2) in the rat testis during postnatal development

Abstract

N-Myc downstream regulated gene 2 (NDRG2) is expressed in the testis of adult animals and is involved in cell differentiation and development. However, little is known about the expression pattern of NDRG2 in the testis during postnatal development. Here, we show that NDRG2 is consistently expressed in Leydig cells in the rat testis during postnatal development. However, its expression has also been detected at a high frequency in spermatogenic cells of the seminiferous tubules in young rats but at a much lower frequency in adult rats. Furthermore, high levels of NDRG2 expression have been found in methoxyacetic-acid-induced apoptotic germ cells, particularly at stages X-XIII of the seminiferous epithelium cycle of adult rats. Interestingly, high levels of NDRG2 expression have also been observed in spontaneously apoptotic germ cells in the seminiferous tubules of young and adult rats. Thus, the expression of NDRG2 in germ cells seems to alter during spermatogenesis. These findings suggest that NDRG2 regulates testicular development and spermatogenesis in rats and is involved in the physiological and pathological apoptosis of germ cells.

Regulated upon activation normal T-cell expressed and secreted originating from the epididymis differentially associates with viable and defective spermatozoa

“…The avidin-biotin technique was used as described (22). After H 2 O 2 treatment, paraffin-embedded sections were blocked for 30 minutes with 10% goat/rabbit serum and incubated with the related anti-RANTES primary antibodies (5 mg/mL) overnight at 4 C, then with the 1:300-diluted biotinylated secondary antibody.…”

Up-Regulation of NDRG2 in Senescent Lens Epithelial Cells Contributes to Age-Related Cataract in Human

“…Inhibition of cell proliferation by overexpression of NDRG2 in malignant cancer cells has also been reported [18], [19], [20]. Accumulating data also suggests that NDRG2 regulates cellular differentiation and development in vitro and in vivo [21], [22], [23], [24], [25], and is involved in cellular response to stress [19], [26].…”

Genome‐wide association study for age at puberty in young Nelore bulls

“…The NDRG2 (NDRG family member 2) gene regulates testicular development and spermatogenesis in rats, which is involved in cell differentiation, physiological and pathological apoptosis of germ cells (Hou et al, ). The SPA17 (sperm autoantigenic protein 17) gene encodes a conserved highly antigenic protein found in acrosome and fibrous sheath of the sperm flagellum, which has been implicated in cell–cell adhesion functions, sperm maturation, capacitation, acrosome reaction and binding of sperm to the zona pellucida of the oocyte (Chiriva‐Internati et al, ; Grizzi et al, ).…”