Differential expression of monocyte/macrophage- selective markers in human idiopathic pulmonary fibrosis

Abstract

Idiopathic interstitial pneumonias are a group of idiopathic interstitial lung diseases of which idiopathic pulmonary fibrosis (IPF) is the lesion of usual interstitial pneumonia. Although the pathogenic mechanisms remain incompletely understood, disease-specific changes in blood, a readily accessible biospecimen, have not been fully characterized. To identify biomarkers from blood and sera, the immune status of IPF patients and control subjects without structural lung disease was quantified by measuring cell surface markers, mRNA levels, and serum proteins. Statistically significant differences in cellular and molecular markers were observed between the 2 groups. The cytokine receptor IL-17RB was significantly higher in CD14+ peripheral blood mononuclear cells (PBMCs) from IPF patients, whereas expression of the chemokine receptor CXCR4 was lower. Gene expression analyses identified 18 differentially expressed genes out of 195 selected. Of these, EMR1, CCR3, UPAR, FCGR2A, OPN, CEACAM3, CD16a, CD18, CD11b, LTF, and LCN2 were up-regulated, whereas IL-17RB, IL-10, PDGFA, CD301/Clec10a, CD25/IL-2RA, IL-23p19, and IL-15 were down-regulated in IPF. Differentially regulated genes were in the functional areas of inflammation and cell signaling. Serum levels of UPAR and OPN were higher in IPF. These observations reveal significant differences in cell and molecular markers involved in monocyte/macrophage activation and migration, and suggest a role for IL-17RB in IPF.

Common and distinct mechanisms of induced pulmonary fibrosis by particulate and soluble chemical fibrogenic agents

“…SPP1 (OPN) has been reported to be induced and to function during wound healing and fibrosis in multiple tissues and organs including the skin, liver, and heart (Leung et al 2013; Matsui et al 2004; Mori et al 2008). The SPP1 level was increased in peripheral blood mononuclear cells and the bronchoalveolar lavage fluid from IPF patients (Desai et al 2011; Pardo et al 2005), as well as mouse lungs exposed to bleomycin (Berman et al 2004). We found that the transcription level of Spp1 was induced by bleomycin or paraquat for more than tenfold; the Spp1 level was also increased by silica for 3.839-fold, though the p value with FDR of silica-treated group was higher than 0.05 (Fig.…”

5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils

“…As shown in Figure 1(a), leukocytes accumulated in response to 50 and 100 ng/cavity eotaxin, in amounts that were significantly different from the RPMI controls (0 ng/cavity) as well as from lower doses of eotaxin (10 and 25 ng/cavity). These leukocytes included variable numbers of eosinophils (Figure 1(b)), monocytes/macrophages [39, 40] (Figure 1(c)), and neutrophils (Figure 1(d)). The morphology of all three leukocyte populations was recognizable without ambiguity, as shown in a representative photomicrograph (supplementary Figure 1 available online at http://dx.doi.org/10.1155/2014/102160).…”

Serological Investigation of the Collagen Degradation Profile of Patients with Chronic Obstructive Pulmonary Disease or Idiopathic Pulmonary Fibrosis

“…Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease characterized by fibroblast proliferation and extracellular remodeling 2,3. Common to both diseases is the highly altered interaction between fibrogenesis and fibrolysis leading to functional impairment of the lungs.…”