Differential expression of miRNAs related to angiogenesis and adipogenesis in subcutaneous fat of obese and nonobese women

Abstract

To disclose the mechanisms surrounding obesity, we selected microRNAs (miRNAs) that target genes involved in adipogenesis, angiogenesis, and inflammation and compared their expression levels in the subcutaneous adipose tissue of 40 obese and nonobese women. Mature miRNAs were extracted from subcutaneous adipose tissue samples that were collected during surgery and quantified by real-time polymerase chain reaction. miR-16 was overexpressed in the nonobese group (n-expression ratio = − 151.1; P < 0.001). Furthermore, the expression levels of two other miRNAs were significantly correlated with waist circumference in nonobese women (miR-27b, r = 0.453; P = 0.027 and miR-424-5p, r = 0.502, P = 0.014). Central and total subcutaneous adipose tissue thicknesses were correlated with miR-424-5p levels (r = 0.506, P = 0.034 and r = 0.475, P = 0.046, respectively) in the nonobese group. In the obese group, miR-424-5p expression was correlated with body mass index (r = 0.582, P = 0.018). miR-16 and miR-424 have shown correlations with body-fat-mass-related parameters. Because these miRNAs have vascular endothelial growth factor (VEGF) and its receptors as target genes, they may be involved in the alterations of angiogenesis observed in obesity. In addition, higher levels of miR-27 and miR-424 were correlated with higher fat depot measurements in nonobese women. These results highlight the importance of miRNA expression in subcutaneous adipose tissue and encourage further investigation of miRNAs as prognostic markers.

CTRP1 decreases ABCA1 expression and promotes lipid accumulation through the miR‐424‐5p/FoxO1 pathway in THP‐1 macrophage‐derived foam cells

“…Extensive studies have demonstrated that abnormal expression of miRNAs is associated with lipid metabolism disorder and atherosclerosis progression (Aryal et al, 2017; Feinberg & Moore, 2016). Emerging evidence suggests that miR‐424‐5p is involved in metabolic diseases, such as diabetes and obesity (Gasparotto et al, 2019; Wang et al, 2020). Interestingly, miR‐424‐5p is lowly expressed in the peripheral blood isolated from patients and rats with atherosclerosis (Li et al, 2020).…”

Obesity related microRNA‑424 is regulated by TNF‑&alpha; in adipocytes

“…A previous study revealed that miR-424 expression levels were closely associated with fat deposition in women (30); hence, in the present study, it was questioned whether a similar expression pattern would be observed in children. In the current study, the expression levels of miR-424 in the adipose tissue of obese and non-obese children were compared.…”

“…Functionally, the expression of miR-424 was identified to be regulated by PPAR-γ, an important transcription factor during adipogenesis (32). Interestingly, in non-obese women, those with higher fat droplet measurements had upregulated expression levels of miR-424 (30). These reports provided novel information about the role of miR-424 in obesity.…”

“…Previous studies have reported the functional relationship between miR-424 and several types of disease and biological processes, including cancer (20)(21)(22)(23), cell differentiation (24), diabetes (25), angiogenesis (26), vascular diseases (27) and inflammatory diseases (28,29). The role of miR-424 in adipogenesis has been studied in recent years, and its expression has been associated with waist-to-hip ratio and to body fat mass related parameters (30,31). Functionally, the expression of miR-424 was identified to be regulated by PPAR-γ, an important transcription factor during adipogenesis (32).…”

Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1

“…miRNA-18b-3p, identified by RNA-seq, inhibits chicken intramuscular adipocyte differentiation by targeting ACOT13 (Sun et al, 2019). Some miRNAs have also been identified as DE-miRNAs by previous RNA-seq studies related to lipid deposition, including miR-20b, miR-424-3p, miR-486-5p, and let-7f-3p (Hosui et al, 2017;Gasparotto et al, 2019;Huang et al, 2019;Ylioja et al, 2019). Interestingly, miR-664a and miR-542-5p were shown to play an important role in skeletal muscle development (Cai et al, 2018;Ji et al, 2020).…”