Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases

Abstract

BackgroundChildren with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profile in heart tissue after cardiac surgery using CPB.MethodsIn total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were employed to determine the miRNA expression profile from three patients before and after connection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools.ResultsAccording to miRNA array, a total of 90 miRNAs were significantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system.ConclusionOur result identified the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The differentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particular.Electronic supplementary materialThe online version of this article (doi:10.1186/s12967-017-1213-9) contains supplementary material, which is available to authorized users.

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

“…The relative abundance level for each miRNA was calculated using the 2 −ΔΔCT equation [23]. RNU6B small nuclear RNA (snRNA) was used an endogenous reference control for normalization purpose as previously described [12,13,22,[24][25][26][27] and because of its minimum abundance variance between the TS patients and HVs as observed by DataAssist™Software (Applied Biosystems). Correlations were computed using Spearman's regression coefficient and the difference between the groups was analyzed using a Mann-Whitney-U test using GraphPad Prism 7.0.…”

Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit+CD45− cell population in newborns

“…Nonetheless, postoperative myocardial stunning and systolic as well as diastolic dysfunction cannot be ruled out with our data. Recently our study group was able to show changes of microRNAs after CPB which are involved in the process of congenital heart defects and myocardial failure [ 27 ]. However, no conclusions of the impact of CPB on the neonatal heart can be drawn and further larger studies need to address this question.…”

Transcriptome analysis defines myocardium gene signatures in children with ToF and ASD and reveals disease-specific molecular reprogramming in response to surgery with cardiopulmonary bypass

“…miR-21, miR22, and miR-23 dysregulated levels have been reported to affect cardiac function and to have potential prognostic and/or therapeutic relevance in various cardiovascular disorders [80,[82][83][84][85], although conflicting results have indicated a cardioprotective role for miR-21 and miR-22 [86,87]. A recent report demonstrated changes in the heart miRNome of CHD patients after CPB [88]. However, our study is the first to provide specific evidence of miR-21, miR-22, and miR-23 upregulation in the post-CPB myocardium, identifying new potential molecular biomarkers and therapeutic targets for future investigations in CHDs.…”