Differential expression of microRNA related to irritable bowel syndrome in a rabbit model

Yang, Qin Qin; Xu, Xiao; Zhao, Hong; Cai, Yue; Pan, Yuming; Xu, Jian; Quan, Xin; Chen, Min Li

doi:10.1111/1751-2980.12485

Cited by 7 publications

(2 citation statements)

References 22 publications

(25 reference statements)

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“…IBS refers to a prevalent disease associated with abdominal pain, altered defecation, as well as slight inflammation and intestinal microbiota changes. 24 Yang et al 25 has indicated that many miRNAs were associated with the occurrence of IBS and increased sensitivity to IBS with a rabbit model. In view of this, the present study explored the anti-inflammatory effect of miR-181c-5p on IBS via IL1A.…”

Section: Discussionmentioning

confidence: 99%

Silencing interleukin 1α underlies a novel inhibitory role of miR‐181c‐5p in alleviating low‐grade inflammation of rats with irritable bowel syndrome

Zhao

et al. 2019

J of Cellular Biochemistry

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Irritable bowel syndrome (IBS) is a common disorder of unknown etiology. Studies have found a close relation between IBS and microRNAs (miRNAs), but the study concerning the relationship between IBS and miR‐181c‐5p in IBS is still blank. Thus, this study aims to explore the role of miR‐181c‐5p in IBS via interleukin 1α (IL1A). Initially, microarray analysis was used to retrieve the genes related to IBS and to predict miRNAs regulating IL1A gene. IBS model was then established with abdominal withdraw reflection (AWR) and Bristol stool grading in mice measured. Afterwards, the functional role of miR‐181c‐5p in IBS was determined using the ectopic expression, depletion and reporter assay experiments, as well as miR‐181c‐5p and IL1A expression detected. Subsequently, expression of tumor necrosis factor‐α (TNF‐α), interleukin‐2 (IL‐2), and IL‐6 were detected to further determine the effects of miR‐181c‐5p and IL1A on inflammation in IBS. miR‐181c‐5p and IL1A might be involved in IBS. miR‐181c‐5p was found to be decreased while IL1A was increased in IBS rats. In addition, miR‐181c‐5p could target and inhibit expression of IL1A, and IBS mice exhibited elevated AWR and Bristol stool grading, namely 6 to 7 points (70.4 [38 of 54]). Moreover, with the overexpression of miR‐181c‐5p or silencing of IL1A, the expression of TNF‐α, IL‐2, and IL‐6 was decreased. Collectively, this study suggested that overexpressed miR‐181c‐5p could silence IL1A, thus inhibiting low‐grade inflammation in IBS rats. miR‐181c‐5p/IL1A is expected to serve as a novel target for the treatment of IBS.

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Section: Discussionmentioning

confidence: 99%

Silencing interleukin 1α underlies a novel inhibitory role of miR‐181c‐5p in alleviating low‐grade inflammation of rats with irritable bowel syndrome

Zhao

et al. 2019

J of Cellular Biochemistry

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“…Therefrom, we choose 21 miRNAs in rats as study targets: let-7f, let-7i, miR-21, miR-23a, miR-23b, miR-24, miR-29a, miR-31a, miR-122-5p, miR-130b, miR-132, miR-143, miR-145, miR-146a, miR-192, miR-199a, miR-200b, miR-217, miR-221, miR-223, and miR-375. 6 12 13 14 15 16 17 18 19 20 21 22 …”

Section: Introductionmentioning

confidence: 99%

Regulation of Colonic Mucosal MicroRNA Expression via Multiple Targets in Visceral Hypersensitivity Rats by Tongxieyaofang

Chao

Wang

et al. 2018

Yonsei Med J

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PurposeThis study aimed to screen for differentially expressed microRNAs (miRNAs) in the colons of rats with visceral hypersensitivity to build the expression profiles of miRNAs therein and to determine the mechanism of Tongxieyaofang use in the treatment of irritable bowel syndrome (IBS).Materials and MethodsForty Sprague-Dawley rats were divided randomly into four groups: control group, model control group (induced by rectum stimulus and evaluated by abdominal withdraw reaction), treatment control group (normal saline), and Tongxieyaofang group (treated with Tongxieyaofang). We screened for differential expression of colonic mucosal miRNAs using liquid chip technology and verified the expression thereof using reverse transcription-PCR.ResultsThe visceral hypersensitivity rat model was successfully established. We found the expression of let-7f, let-7i, miR-130b, miR-29a, miR-132, miR-21, and miR-375 to be up-regulated (p<0.05), while the expression of miR-24, miR-31a, miR-192, miR-221, and miR-223 was down-regulated (p<0.05) in the visceral hypersensitivity rats. After treatment with Tongxieyaofang, the expression of let-7f, let-7i, miR-130b, miR-29a, miR-132, miR-21, and miR-375 was reduced (p<0.05), whereas the expression of miR-24, miR-31a, miR-192, miR-221, miR-223 was increased, compared to the treatment control group (p<0.05).ConclusionMiRNAs play a pivotal role in visceral hypersensitivity and might be targets in the treatment of IBS by Tongxieyaofang.

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High-throughput sequencing reveals microRNAs in response to heat stress in the head kidney of rainbow trout (Oncorhynchus mykiss)

Liu

Huang

et al. 2019

Funct Integr Genomics

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Differential expression of microRNA related to irritable bowel syndrome in a rabbit model

Abstract: Twelve miRNAs were differentially expressed in IBS rabbits. Five are specific in WHBE IBS rabbits, suggesting that they play a role in increased sensitivity to IBS.

Cited by 7 publications

References 22 publications

Silencing interleukin 1α underlies a novel inhibitory role of miR‐181c‐5p in alleviating low‐grade inflammation of rats with irritable bowel syndrome

Silencing interleukin 1α underlies a novel inhibitory role of miR‐181c‐5p in alleviating low‐grade inflammation of rats with irritable bowel syndrome

Regulation of Colonic Mucosal MicroRNA Expression via Multiple Targets in Visceral Hypersensitivity Rats by Tongxieyaofang

High-throughput sequencing reveals microRNAs in response to heat stress in the head kidney of rainbow trout (Oncorhynchus mykiss)

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