Differential Expression of Metabolism-related Genes in Liver of Diabetic Obese Rats

Seo, Eunhui; Park, Eun Jin; Park, Mikyoung; Kim, Duk Kyu; Lee, Hye Jeong; Hong, Sook-Hee

doi:10.4196/kjpp.2010.14.2.99

Cited by 10 publications

(8 citation statements)

References 16 publications

(14 reference statements)

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“…Hyperinsulinaemia, as observed in pre-and overt diabetes, may therefore be associated with induction of SCD1. Consistent with this, Seo et al [64] show that obese, hyperinsulinaemic Otsuka Long-Evans Tokushima Fatty rats have significantly up-regulated hepatic SCD1 expression compared to control rats. ERK1/2 phosphorlyation also appears to promote the activity sterol regulatory element binding protein (SREBP) [65], with Kotzka et al [66] providing evidence for this in hepatic hepG2 cells.…”

Section: Discussionsupporting

confidence: 67%

Diet-induced Obesity Up-regulates the Abundance of GPR43 and GPR120 in a Tissue Specific Manner

Cornall

Mathai

Hryciw

et al. 2011

Cell Physiol Biochem

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Background/Aims: GPR43 and GPR120 have recently been deorphanised as receptors for fatty acids. Fatty acids mediate a variety of metabolic processes in the body, however, the effect these receptors have on metabolism is not fully understood. Here, we characterise the effect of diet-induced obesity on the expression of GPR43 and GPR120 in tissues important in maintaining metabolic health. Methods: Six-week old male Sprague Dawley rats were fed either a high fat diet (HFD; 22% fat) or control diet (5% fat; n = 8-9/group) for 12 weeks. Rats were euthanized and the heart, liver, soleus and extensor digitorum longus (EDL) skeletal muscles were excised. GPR43 and GPR120 receptor abundance was quantified by ‘real-time’ PCR. Results: GPR43 mRNA abundance was significantly up-regulated by a HFD in liver and soleus and EDL skeletal muscles compared to control (p ≤ 0.05). Whilst a HFD significantly up-regulated GPR120 gene transcripts in cardiac tissue and EDL skeletal muscle when compare to control (p ≤ 0.05). Conclusion: We have shown for the first time that up-regulation of GPR43 and GPR120 in response to a HFD, is tissue specific. This suggests these receptors have different roles in mediating metabolic function in a number of tissues in the human body.

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Section: Discussionsupporting

confidence: 67%

Diet-induced Obesity Up-regulates the Abundance of GPR43 and GPR120 in a Tissue Specific Manner

Cornall

Mathai

Hryciw

et al. 2011

Cell Physiol Biochem

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“…To better understand the biological mechanisms underlying excessive alcohol use among adolescents and young adults in regard to prediabetes and early diabetes, we studied Otsuka Long-Evans Tokushima Fatty (OLETF) rats (11~22 weeks of age), which have prediabetes and early diabetes induced by excessive levels of alcohol. The clinical and pathological features of OLETF rats resemble those of humans with type 2 diabetes [ 16 17 18 ].…”

Section: Introductionmentioning

confidence: 99%

Chronic Alcohol Consumption Results in Greater Damage to the Pancreas Than to the Liver in the Rats

Lee

Hong

et al. 2015

Korean J Physiol Pharmacol

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Alcohol consumption increases the risk of type 2 diabetes. However, its effects on prediabetes or early diabetes have not been studied. We investigated endoplasmic reticulum (ER) stress in the pancreas and liver resulting from chronic alcohol consumption in the prediabetes and early stages of diabetes. We separated Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a type-2 diabetic animal model, into two groups based on diabetic stage: prediabetes and early diabetes were defined as occurrence between the ages of 11 to 16 weeks and 17 to 22 weeks, respectively. The experimental group received an ethanol-containing liquid diet for 6 weeks. An intraperitoneal glucose tolerance test was conducted after 16 and 22 weeks for the prediabetic and early diabetes groups, respectively. There were no significant differences in body weight between the control and ethanol groups. Fasting and 120-min glucose levels were lower and higher, respectively, in the ethanol group than in the control group. In prediabetes rats, alcohol induced significant expression of ER stress markers in the pancreas; however, alcohol did not affect the liver. In early diabetes rats, alcohol significantly increased most ER stress-marker levels in both the pancreas and liver. These results indicate that chronic alcohol consumption increased the risk of diabetes in prediabetic and early diabetic OLETF rats; the pancreas was more susceptible to damage than was the liver in the early diabetic stages, and the adaptive and proapoptotic pathway of ER stress may play key roles in the development and progression of diabetes affected by chronic alcohol ingestion.

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“…Long-term insulin resistance may cause type 2 diabetes and a subsequent increase in morbidity and impaired glucose tolerance [ 7 ]. Thus, controlling obesity is critical for the reduction of future health problems and morbidity [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Yoga Training Improves Metabolic Parameters in Obese Boys

Seo

Lee

Figueroa

et al. 2012

Korean J Physiol Pharmacol

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Yoga has been known to have stimulatory or inhibitory effects on the metabolic parameters and to be uncomplicated therapy for obesity. The purpose of the present study was to test the effect of an 8-week of yoga-asana training on body composition, lipid profile, and insulin resistance (IR) in obese adolescent boys. Twenty volunteers with body mass index (BMI) greater than the 95th percentile were randomly assigned to yoga (age 14.7±0.5 years, n=10) and control groups (age 14.6±1.0 years, n=10). The yoga group performed exercises three times per week at 40~60% of heart-rate reserve (HRR) for 8 weeks. IR was determined with the homeostasis model assessment of insulin resistance (HOMA-IR). After yoga training, body weight, BMI, fat mass (FM), and body fat % (BF %) were significantly decreased, and fat-free mass and basal metabolic rate were significantly increased than baseline values. FM and BF % were significantly improved in the yoga group compared with the control group (p<0.05). Total cholesterol (TC) was significantly decreased in the yoga group (p<0.01). HDL-cholesterol was decreased in both groups (p<0.05). No significant changes were observed between or within groups for triglycerides, LDL-cholesterol, glucose, insulin, and HOMA-IR. Our findings show that an 8-week of yoga training improves body composition and TC levels in obese adolescent boys, suggesting that yoga training may be effective in controlling some metabolic syndrome factors in obese adolescent boys.

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Differential Expression of Metabolism-related Genes in Liver of Diabetic Obese Rats

Cited by 10 publications

References 16 publications

Diet-induced Obesity Up-regulates the Abundance of GPR43 and GPR120 in a Tissue Specific Manner

Diet-induced Obesity Up-regulates the Abundance of GPR43 and GPR120 in a Tissue Specific Manner

Chronic Alcohol Consumption Results in Greater Damage to the Pancreas Than to the Liver in the Rats

Yoga Training Improves Metabolic Parameters in Obese Boys

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