Differential expression of maize mitochondrial genes as dependent on mitochondria redox state

The role of electron transport in the defence response of the South African abalone, Haliotis midae

“…For example, superoxide produced in haemocytes from the white shrimp, Penaeus vannamei, that had not been stimulated by various disease response elicitors was attributed as background superoxide [24]. Production of high levels of superoxide due to antimycin A treatment has been widely demonstrated in various cell types [6,12,25,26] and organisms [27]. Meaney et al [13] showed that mitochondria treated with antimycin A released five times more superoxide into the mitochondrial matrix compared to control samples.…”

Modified alternative oxidase expression results in different reactive oxygen species content in Arabidopsis cell culture but not in whole plants

“…To extract total RNA, suspension culture cells were homogenized with liquid nitrogen, then 0.45 cm 3 of TE buffer, 0.05 cm 3 SDS (10 %), and 0.5 cm 3 of phenol were added. Further extraction was performed as previously described (Garnik et al 2006). Total RNA from plants was extracted by TRI reagent (Sigma-Aldrich, St. Louis, USA) according to manufacturer's instructions.…”

Expression of genes encoding subunits of respiratory complexes in Arabidopsis leaves depends on light

“…Total cellular RNA was separated in a 1.2% agarose gel under denaturing conditions and then transferred onto a Hybond N nylon membrane (Amersham, United States) [10]. Prehybridization (42°C, 60 min) was performed in a buffer containing 5× SSC, 5× Den hardt's solution, 0.5% SDS, and 50% formamide.…”