Differential Expression of Long Noncoding RNAs in Patients with Coronary Artery Disease

Abstract

Long noncoding RNAs (lncRNAs) constitute the largest class of noncoding RNAs and play significant roles in the development of cardiovascular pathologies. In the present study, we aimed to evaluate whether 4 candidate lncRNAs – MIAT, MEG3, MALAT1, and MCM3AP-AS1 – have distinct expression levels in patients with obstructive coronary artery disease (CAD) and reveal the diagnostic and therapeutic potentials of these lncRNAs for CAD. A total of 90 patients who subjected to coronary angiography were enrolled. Relative expression of lncRNAs were assayed using qRT-PCR methodology. As a result, <i>MIAT</i> was downregulated, while <i>MEG3</i> was upregulated in CAD patients. Receiver operating characteristic curves demonstrated that these lncRNAs have a high potential to provide sensitive and specific diagnosis of CAD. The calculated area under curve levels indicated that MIAT and MEG3 have high diagnostic value for detecting the presence of significant CAD. However, <i>MALAT1</i> and <i>MCM3AP-AS1</i> levels were not sufficiently reliable for CAD development in our cases. Here, we demonstrate that <i>MIAT</i> and <i>MEG3</i> were differentially expressed in our patients and might be promising biomarkers and therapeutic targets for CAD. These results indicate that <i>MIAT</i> and <i>MEG3</i> could play chief roles in CAD development.

Association of Angio-LncRNAs MIAT rs1061540/MALAT1 rs3200401 Molecular Variants with Gensini Score in Coronary Artery Disease Patients Undergoing Angiography

“…Numerous biological processes, such as epigenetic modifications, chromatin remodeling, splicing, and cellular differentiation, have been linked to lncRNAs, solidifying their status as critical genetic regulators [ 6 ]. Accumulating evidence indicates that lncRNA can play key roles in CAD, including disease diagnosis and/or prognosis [ 7 , 8 , 9 , 10 ].…”

Long Non-Coding RNA MCM3AP-AS1: A Crucial Role in Human Malignancies

“…The functions of MCM3AP-AS1 are diverse and are mainly divided into those in neoplastic diseases and those in non-neoplastic diseases. In non-neoplastic diseases, MCM3AP-AS1 promotes chondrocyte apoptosis, promotes the progression of hemangiomas, regulates the proliferation of human bronchial smooth muscle cells, promotes osteogenic differentiation of dental pulp stem cells, and is associated with acute stroke and coronary artery disease [ 18 – 23 ]. In most tumors, MCM3AP-AS1 mainly shows pro-cancer effects, such as promoting tumor cell proliferation, inhibiting apoptosis and promoting cell metastasis.…”