Differential expression of litchi XET genes in relation to fruit growth

Lu, Wang-jin; Wang, Y.; Jiang, Yueming; Li, J.; Liu, H.; Duan, Xuewu; Song, Lili

doi:10.1016/j.plaphy.2006.09.020

Cited by 46 publications

(33 citation statements)

References 32 publications

(39 reference statements)

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“…They are hypothesized to disturb the hydrogen bonds between cellulose microfibers and cross-linking matrix glycans, resulting in slippage between cell walls. Besides expansins, there are many enzymes, such as xyloglucan endotransglycosylases (XETs) (Nishikubo et al 2011;Stratilová et al 2010;Lu et al 2006;Johansson et al 2004;Bourquin et al 2002) and endo-1,4-D-glucanases (EGs), which are also confirmed to have an effect on cell expansion.…”

Section: Discussionmentioning

confidence: 99%

OsEXPB2, a β-expansin gene, is involved in rice root system architecture

et al. 2015

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Expansins are cell wall loosening proteins which generally play important roles as endogenous regulators in plants. Here we report a rice (Oryza sativa L.) b-expansin gene, OsEXPB2, which encodes a 28.6-kDa protein of 261 amino acids. Sequence alignment revealed that the N-terminal and C-terminal of OsEXPB2 share six discontinuous cysteine residues and four intermittent tryptophan residues, respectively. The OsEXPB2 promoter contains conserved root hair-specific elements. Subcellular localization assay revealed that OsEXPB2 was localized in the cell wall. Analysis of spatial and temporal expression patterns demonstrated that OsEXPB2 was predominantly expressed in root of rice. OsEXPB2 expression levels were up-regulated by abiotic stresses, such as phosphate or iron deficiency, and also suppressed by abscisic acid. A clear difference was observed between RNA interference (RNAi) lines and wildtype in root system architecture and plant height, and the suppression of OsEXPB2 resulted in a visible alteration of the width of the leaf blade. Anatomical analysis found that the cell size of root cortical cells in OsEXPB2-suppressed lines was significantly smaller than that of their counterparts in wild-type plants. Furthermore, cryo-scanning electron microscopy analysis showed that the development of root hair was suppressed in RNAi lines. All these results suggest that OsEXPB2 is a root-predominant gene with a key role in root-hair formation and has the potential to be utilized in transgenic root breeding to improve abiotic stress tolerance.

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Section: Discussionmentioning

confidence: 99%

OsEXPB2, a β-expansin gene, is involved in rice root system architecture

et al. 2015

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“…Tm-NXG1, on the other hand, is expressed exclusively in this tissue, from which the active enzyme has been isolated (Edwards et al, 1986). Furthermore, Tm-NXG1 homologs are expressed specifically in a range of ripening fruits, including Litchi (Lu et al, 2006), papaya (Carica papaya) (GenBank accession number AY032600; R. Haireen, Z.M. Ali, R. Othman, and H. Lazan, unpublished data), grape (Vitis vinifera) (Ishimaru and Kobayashi, 2002), and apricot (Prunus armeniaca) (GenBank accession number AY578086; F. Geuna, R. Banfi, and D. Bassi, unpublished data).…”

Section: Biological Implicationsmentioning

confidence: 99%

“…Ali, R. Othman, and H. Lazan, unpublished data), Tropaeolum majus (Tm-NXG1) (de Silva et al, 1993), Litchi chinensis (Lc-XET1) (Lu et al, 2006), and Vitis labrusca 3 Vitis vinifera (Vv-XET1) (Ishimaru and Kobayashi, 2002), selected from curated CarbohydrateActive Enzyme database entries (http://www.cazy.org). Full-length Solanum lycopersicum ESTs (16 sequences) were selected from those reported by Saladié et al (2006).…”

Section: Bioinformatic Analysismentioning

confidence: 99%

Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan Endo-Transglycosylases: Biological Implications for Cell Wall Metabolism

et al. 2007

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High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure–function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen (Populus tremula × Populus tremuloides). Production of the loop deletion variant Tm-NXG1-ΔYNIIG yielded an enzyme that was structurally similar to Ptt-XET16-34 and had a greatly increased transglycosylation:hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.

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“…Results from the blast analysis demonstrate that its amino acid sequence was highly homologous to Arabidopsis thaliana, Asparagus officinalis, Cucumis sativus, Litchi chinensis and Populus tremula × Populus tremuloides. This proved Previous studies have shown that various members of the XET families had different functions, such as loosening cell walls, elongating some plant tissues (Fry, 1998), softening fruits (Lu et al, 2006) or forming secondary vascular tissues (Reidy et al, 2001). Recent studies have shown that XETs played roles in the synthesis of cell wall polysaccharides, the regulation of cell-wall loosening and assembling the polysaccharides on intermicrofibriles (Herbers et al, 2001) during cell growth.…”

Section: Discussionmentioning

confidence: 71%

Cloning of XET gene from Anthocephalus chinensis and its plant expression vector construction

Zhu

et al. 2010

For. Stud. China

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A full-length cDNA sequence of xyloglucan endotransglycosylase gene (XET), abundantly expressed in the cambium of Anthocephalus chinensis was cloned by conserved PCR, rapid-amplification of cDNA ends and by chromosome walking. Analytical results of the DNA sequence show that a 912 bp complete open reading frame (ORF) encoded a 303-amino acid protein was in the 1205 bp full cDNA sequence. The deduced amino acid sequence of AcXET, which contained the conserved specific EIDFE catalytic site sequence to XETs was homologous to the other known XET proteins. In order to study the gene function of AcXET and obtain transgenic plants, a plant expression vector pBIAcXET was constructed by recombinating the AcXET fragment from the cloning vector pMD19AcXET and the binary vector pBI121 between the XbaI and SmaI sites. The fragment of AcXET gene was inserted between the CaMV 35S promotor and the coding region of the GUS gene in pBI121. The identification results show that the plant expression binary vector pBIAcXET was constructed successfully. These results lay the foundation for studying the molecular mechanism of AcXET gene during wood formation.

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Differential expression of litchi XET genes in relation to fruit growth

Cited by 46 publications

References 32 publications

OsEXPB2, a β-expansin gene, is involved in rice root system architecture

OsEXPB2, a β-expansin gene, is involved in rice root system architecture

Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan Endo-Transglycosylases: Biological Implications for Cell Wall Metabolism

Cloning of XET gene from Anthocephalus chinensis and its plant expression vector construction

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