Differential Expression of Inflammation-related Genes after Intense Exercise

Abstract

Abstract:The present study focused on the identification of the difference in expression of inflammation-related genes after intense exercise by oligonucleotide microarray methods. This may finally lead to an improved understanding of underlying cellular and molecular mechanism of the immunological alterations in response to exercises. The study group consisted of three healthy road cyclists. Peripheral blood mononuclear cells (PBMCs) were collected preexercise, immediately post-exercise and after 15 min of recovery. The analysis of the expression profile of genes related to the inflammation was performed in PBMCs using HG-U133A oligonucleotide microarrays. 4 genes were found to be regulated by more than 2.0-fold (IL1R2, IL2RB, IL8, IL8RB). Venn diagram indicated that only one of differentially expressed genes (TXLNA) remains the same in each comparison. The balance of both pro-and anti-inflammatory cytokines after exercise seems to be important for athletes. Optimal inflammatory and immune response may help optimize exercise regimes, link physical activity with health and diagnose or prevent athletes from overtraining.

Effects of Exercise on Gene Expression of Inflammatory Markers in Human Peripheral Blood Cells: A Systematic Review

“… 69 ↑ after MT (of which 64 were identical to RTE) Pathways regulated were related to inflammation, stress response, signal transduction, and apoptosis. Kimsa et al [ 44 ] Ergometric cycling and expression pattern of inflammation-related genes. Blood samples were drawn before exercise (pre-ex), immediately after exercise (post-ex), and 15 min into recovery.…”

“…When using this approach, Kimsa et al found that an acute bout of bicycling regulated several biological pathways such as cytokine-mediated signaling pathways (IL6, IL8, and IL1B), intracellular signaling (IL5RA, IL6, and IL8), cell communication and cell-to-cell signaling (interleukin 2 receptor beta (IL2RB), colony-stimulating factor 2 (CSF2), and interleukin 1 receptor-like 1 (IL1R1). In total, ten inflammation-related genes were changed after exercise [ 44 ].…”

Effect of Lower and Upper Body High Intensity Training on Genes Associated with Cellular Stress Response

“…Changes in genes associated with inflammation and HSP show the result of alterations in signalling via these pathways. There is considerable evidence demonstrating the influence of various exercise types on inflammation [ 13 , 14 ] and gene expression of heat shock proteins [ 15 , 16 ], thereby mediating the health benefits of episodic and prolonged exercise.…”

“…The health-promoting effects of exercise are associated with interleukin production, eliciting the anti-inflammatory response by decreasing inflammation [ 10 ] and increasing stress tolerance. Therefore, determining the balance of pro- and anti-inflammatory cytokines after exercise is important, not only in sports, but also in terms of health [ 13 ]. Additionally, the type of training is one of many factors that can influence the adaptive effect to exercise.…”

Estudo da via de sinalização da apoptose de neutrófilos em atletas praticantes de meia maratona suplementados ou não com óleo de peixe.

“…Em humanos, durante e após as sessões de exercício físico, alguns leucócitos como linfócitos, macrófagos e células natural killer (NK) apresentam alterações funcionais e metabólicas comprometendo as respostas imunológica e inflamatória (GLEESON, 2007;KIMSA et al, 2014;MORO-GARCIA et al, 2014), principalmente nos exercícios físicos realizados de modo intenso e extenuante (MALM, 2006). Na competição de maratona, as alterações mais evidentes ocorrem nas células NK, macrófagos e neutrófilos (MOOREN et al, 2012;NIEMAN, 1997).…”