Differential Expression of IL-10 Gene and Protein in Target Tissues of Rattus Norvegicus Strain Wistar Model Type 2 Diabetes Mellitus (T2DM)

Bare, Yohanes; Marhendra, Agung Pramana Warih; Sasase, Tomohiko; Fatchiyah, Fatchiyah

doi:10.5455/aim.2018.26.87-92

Cited by 16 publications

(16 citation statements)

References 24 publications

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“…When inflammation occurs, the body will respond by forming anti-inflammatory cytokines to produce symptoms of inflammation. Bare et al, (2018) in the previous study reported the protein profile of all tissues in inflammation (type 2 diabetes mellitus) group and the normal group was completely diverse as proper by Experion Pro260 analysis besides in SDS-PAGE analysis show same results.…”

Section: Introductionmentioning

confidence: 68%

In Silico Insight the Prediction of Chlorogenic Acid in Coffee through Cyclooxygenase-2 (COX2) Interaction

Bare¹,

Sari

Rachmad³

et al. 2019

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Inflammation was signs of pathological or abnormality in tissue to give an alert as a trouble signal to the system. Therapeutic using NSAIDs has some side effects. This research explored the potential role of chlorogenic acid as natural therapeutic compound to inhibit the inflammation target such as COX-2 by interaction model. The research method used in this study was the molecular docking approach, which binds ligand and protein. Protein data provided by Protein Data Bank (ID: 6cox) while, chlorogenic acid obtain from PubChem (CID: 1794427). We docked COX-2 and chlorogenic acid using Hex 8.0.0. Visualization and analysis of the molecular interactions of chlorogenic acid and COX-2 conducted by the Discovery Studio Client 4.1 software. Chlorogenic acid has a high permeability and is easily absorbed based on five Lipinski Rule. Interestingly, we found Fifteen amino acid was binding with chlorogenic acid that formed by hydrogen bond and van der Waals.The interaction between ligand-protein results in energy binding -327.59cal/mol. Chlorogenic acid has a potential role to inhibit inflammation pathway by inhibiting COX-2. We predicted chlorogenic acid has a potential as therapy anti-inflammatory to suppress COX-2 as mediator inflammation.

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Section: Introductionmentioning

confidence: 68%

In Silico Insight the Prediction of Chlorogenic Acid in Coffee through Cyclooxygenase-2 (COX2) Interaction

Bare¹,

Sari

Rachmad³

et al. 2019

bio

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“…Hypertension is one of factor to Type 2 Diabetes Mellitus. In Case of high glucose showed has different profile protein compare with normal conditions (case of Type 2 Diabetes Mellitus) Bare, Marhendra, Sasase, & Fatchiyah, 2018).…”

Section: E E L L --H H a A Y Y A A H H 1 Introductionmentioning

confidence: 93%

In Silico Study: Prediction the Potential of Caffeic Acid As ACE inhibitor

Bare¹,

Kuki²,

Tiring³

et al. 2020

el–Hayah

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Hypertension is an abnormal increase in blood pressure. Regulating blood and cardiovascular function have correlated with the ACE pathway. To decrease blood pressure can use the ACE inhibitor. This paper aims to predict potential of Caffeic Acid as anti-hypertension by blocking the ACE pathway. The method in this research used in silico study. The protein was obtained from Protein Data Bank (PDB) and the ligand was obtained from PubChem. Molecular docking was performed by using HEX and visualization analysis was analyzed by using Discovery Studio. The interaction of caffeic acid and ACE has a functional as anti-hypertension roles. The evidence by twelve amino acid, which bind with the caffeic acid (ASP377, ASN277, ASN285, GLU376, ALA170, ASN167, ASN374, THR372, THR166, CYS370, GLU162 and PRO163). The chemistry bond was formed are hydrogen bond, van der Waals and electrostatics in amino residue ASP377. This binding could stop the synthesis of AT-I to AT-II which pathway to hypertension. Caffeic acid has a potential role as anti-hypertension by inhibiting ACE.

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“…Sistem imun merespon dengan melepaskan mediator sitokin antiinflamasi seperti IL-10 untuk menekan inflamasi yang yang terjadi di dalam tubuh. Akan tetapi penelitian Bare, Marhendra, Sasase, & Fatchiyah, (2018) melaporkan pada saat inflamasi gen IL-10 mengalami mutasi pada organ lambung dan otak. Mutasi yang terjadi menyebabkan menurunkan kinerja IL-10 sebagai anti-inflamasi.…”

Section: Pendahuluanunclassified

Prediksi Asam Kuinat Sebagai Anti-Inflamasi Terhadap COX-2 Secara Virtual

Bare¹,

Kuki²,

Rophi³

et al. 2019

Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati

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Inflamasi merupakan mekanisme pertahanan tubuh terhadap terhadap rangsangan berbahaya, seperti patogen, sel-sel yang rusak, senyawa beracun, atau iradiasi. Selama inflamasi dalam tubuh terdapat COX-2 mediator inflamasi yang peran meningkatkan inflamasi. Sistem imun anti-inflamasi yang mengalami mutasi menyebabkan inflmasi meningkat. Oleh karena itu untuk menurnkannya menggunakan bioaktif alam. Asam kuinat memiliki toksisitas yang sangat rendah dan tidak memberikan efek negatif terhadap organ tubuh manusia. Asam kuinat memiliki potensi yang besar sebagai kandidat obat tertinggi dalam terapi. Akan tetapi kurangnya kajiannya. Penelitian ini bertujuan unutk memprediksi potensi serta menganalisis asam kuinat sebagai agen inflamasi dengan cara menghambat COX-2. Metode yang digunakan terdiri atas pengunduhan protein COX-2 dari protein data bank (PDB) dan asam kuinat diperoleh dari database PubChem, persiapan protein (COX-2) dan ligan (asam Kuinat) dengan program PyRx, analisis interaksi protein dan ligan menggunakan program Hex 8.0.0 dan Discovery Studio client 4. Interaksi antara protein dan ligan menunjukan hasil positif dengan ditemukan 2 domain protein yang berikatan dengan asam kuinat. Protein domain A (GLU140, ASN144, SER143, dan TRP139) dan protein domain B (GLU236, THR237, LYS333, GLN241, GLN330, PHE329, dan LEU238). Ikatan yang terbentuk ada ikatan hidrogen dengan energi sebesar -198.95cal/mol. Asam kuinat diprediksi memiliki potensi sebagai terapi anti-inflamasi, hal ini ditunjukan karena ada ikatan yang terbentuk antara ligan dan 11 residu asam amino.

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Differential Expression of IL-10 Gene and Protein in Target Tissues of Rattus Norvegicus Strain Wistar Model Type 2 Diabetes Mellitus (T2DM)

Cited by 16 publications

References 24 publications

In Silico Insight the Prediction of Chlorogenic Acid in Coffee through Cyclooxygenase-2 (COX2) Interaction

In Silico Insight the Prediction of Chlorogenic Acid in Coffee through Cyclooxygenase-2 (COX2) Interaction

In Silico Study: Prediction the Potential of Caffeic Acid As ACE inhibitor

Prediksi Asam Kuinat Sebagai Anti-Inflamasi Terhadap COX-2 Secara Virtual

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