Differential expression of Ia molecules by human monocytes.

Gonwa, Thomas A.; Stobo, John D.

doi:10.1172/jci111503

Cited by 56 publications

(18 citation statements)

References 47 publications

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“…This has been shown in several previous studies (10,11,31) and was confirmed also in the rheumatoid situation in the present investigation. While entering the synovial tissue, blood monocytes apparently receive activation and/or differentiation signals that lead to an enhanced DR antigen expression as well as to a newly acquired expression of DQ antigens.…”

Section: Discussionsupporting

confidence: 93%

“…This is suggested by the findings that not all Ia molecules are expressed in equal amounts on the cell surface. Thus, a divergent expression of DR and DQ class II molecules has been described on peripheral blood monocytes (10) and certain tissue cells (11). In addition, investigating the differential Ta antigen expression of synovial cells after incubation with Interferon-y, which has recently been shown to be a potent Ta antigen inducer on these cells ( 12), may shed light on a possible role of this mediator in an in vivo situation.…”

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confidence: 99%

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Differential expression of Ia antigens by rheumatoid synovial lining cells.

Burmester¹,

Jahn²,

Rohwer³

et al. 1987

J. Clin. Invest.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Differential expression of Ia antigens by rheumatoid synovial lining cells.

Burmester¹,

Jahn²,

Rohwer³

et al. 1987

J. Clin. Invest.

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“…A similar predominance of HLA-DR expression has been noted on other cell types including monocytes (23), Kupffer cells (24), and synovial lining cells (25). Differential regulation of expression of these two MHC class II proteins in HL-60 myelomonocytic cells has been noted (26), but the mechanism and significance of the predominant HLA-DR expression by eosinophils, as well as by other cell types, are not defined.…”

Section: Methodsmentioning

confidence: 75%

Mature human eosinophils have the capacity to express HLA-DR.

Lucey

Nicholson‐Weller

Weller

1989

Proc. Natl. Acad. Sci. U.S.A.

145

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Human eosinophils are known to lose Ia antigen expression as they mature, and, accordingly, eosinophils obtained from the blood of five eosinophilic donors and three of four normal donors failed to display the major histocompatibility complex class II antigen HLA-DR, as determined by flow cytometry. However, when eosinophils from these nine donors were maintained in culture with recombinant human granulocyte-macrophage colony-stimulating factor and murine 3T3 fibroblasts, HLA-DR consistently developed on the eosinophils. By days 4-6 of culture, 24-97% ofeosinophils were HLA-DR+, and the eosinophils remained morphologically mature. In contrast, another class H antigen, HLA-DQ, was not detectable by flow cytometry on eosinophils from eight of nine donors. Cultured eosinophils were able to synthesize Culture of Eosinophils. Enriched eosinophils (1.5 x 106) were cultured in 3 ml of RPMI 1640 medium containing 10%6 (vol/vol) fetal calf serum and 150 units (=50 pM) of rhGM-CSF (expressed in yeast and purified to homogeneity; Genzyme) with an adherent monolayer of Swiss 3T3 fibroblasts (American Type Culture Collection) at 370C (5% C02/95% air) as described (7); 1.5 ml of culture medium was exchanged with fresh RPMI 1640/10% fetal calf serum/rhGM-CSF on days 2, 5, and 7. Nonadherent cells were collected on the indicated days; prior to flow cytometry, cell viability (>90%) was assessed with trypan blue and cell morphology was assessed by staining with phloxine-methylene blue and fast green-neutral red stains for eosinophils (8).Flow Cytometric Analyses. Flow cytometry (FACScan;Becton Dickinson) of 104 cells was performed after 5 x 10' eosinophils were stained with either 10 ,ul of fluorescein isothiocyanate-conjugated monoclonal antibodies for HLA-DR or CD5 (anti-Leu-1), both IgG K2a light chain (Becton Dickinson). Antigen expression was also assessed with fluorescein isothiocyanate-conjugated monoclonal antibodies (Becton Dickinson) for HLA-DQ, CD16 (anti-Leu-lla), and anti-Leu-M3. For comparison, HLA-DR expression was also evaluated on activated monocytes that had been isolated from peripheral blood, purified by adherence, and incubated for 24 hr with y-interferon (500 units, Sigma) as described (9).

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“…It has been reported that the average number of HLA-DR molecules per macrophage is 120,OOO/cell (163). The number ofHLA-DR molecules per cell or DRbearing macrophage in tuberculosis is unknown, and it is not known if there is a critical density of DR molecules for macrophage function.…”

Section: Discussionmentioning

confidence: 99%