Differential Expression of Hypertension-Associated MicroRNAs in the Plasma of Patients With White Coat Hypertension

Abstract

White coat hypertension (WCH) is a high cardiovascular risk condition, and a fundamental understanding of the cause and pathophysiology of the disorder is still lacking. Recent studies demonstrated that microRNAs (miRNAs) are involved in hypertension; however, the roles of miRNAs in WCH are not known.The expressions of selected 10 miRNAs were investigated independently in plasma samples from 30 hypertension (HT) patients, 30 WCH patients, and 30 normotensive (NT) subjects.MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively). MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively). MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively).Additionally, the ambulatory 24-hour and daytime systolic and diastolic blood pressures were negatively correlated with miR-296-5p. MiR-296 and miR-637 had area under the curve (AUC) values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals. MiR-296 and miR-637 had AUC values of 0.868 and 0.680, respectively, which shows their potential to distinguish WCH from HT individuals.We report for the first time a plasma miRNA profile for WCH patients and demonstrate a novel link between miRNA and WCH. These findings may reveal crucial insights into the development of WCH.

Up‐regulation of circular RNA hsa_circ_0037909 promotes essential hypertension

“…It could act as a suppressor regulated on follicular thyroid carcinomas, ovarian cancer, and hepatocellular carcinoma, and it also participated in the differentiation of cells, such as human mesenchymal stem cells, migration and invasion of glioma cell, and human pancreatic ductal adenocarcinoma cells via suppressing Akt1 expression . At the same time, hsa‐miR‐637 can performed as a marker of follicular thyroid cancer, even hypertension . In the ATP6V0A1 polymorphism, the miR‐637 expression has been associated with EH development .…”

MicroRNAs and Endothelial (Dys) Function

“…A recent study aiming to investigate the differential expression of hypertension‐associated miRs in the plasma of patients with white coat hypertension revealed that miR‐21, miR‐122, miR‐637, and let‐7e are up‐regulated in hypertensive subjects, whereas miR‐122 and miR‐637 are higher in white‐coat patients than in control subjects (Cengiz et al, ). BP values were negatively correlated with miR‐296‐5p.…”

“…Moreover, high titres of CMV have been found in hypertensive patients, leading to the speculative conjecture that CMV could contribute to hypertension by modulating miR pathways. A recent study aiming to investigate the differential expression of hypertension-associated miRs in the plasma of patients with white coat hypertension revealed that miR-21, miR-122, miR-637, and let-7e are up-regulated in hypertensive subjects, whereas miR-122 and miR-637 are higher in whitecoat patients than in control subjects (Cengiz et al, 2015). BP values were negatively correlated with miR-296-5p.…”

Circulating microRNA let‑7e is decreased in knee osteoarthritis, accompanied by elevated apoptosis and reduced autophagy

“…As a tumor suppressor microRNA, the plasma levels of let-7e are downregulated in malignant germ cell tumors, retinoblastoma, and esophageal adenocarcinoma (5)(6)(7). Let-7e is also associated with cardiovascular diseases; let-7e expression levels were significantly upregulated in patients with hypertension (8) and ischemic stroke during the acute stage (9), and downregulated in patients with ventricular septal defect (10). In addition, let-7e is associated with the number of metabolic syndrome Circulating microRNA let-7e is decreased in knee osteoarthritis, accompanied by elevated apoptosis and reduced autophagy traits (11), and the expression levels of let-7e are significantly increased in patients with Hashimoto's disease (12).…”