Differential expression of HLA‐DR and DR‐linked determinants on human leukemias and lymphoid cells

Abstract

Leukemic and normal hemopoietic cells were examined with the monoclonal anti-bodies DA2 and Genox 353 for the presence of HLA-DR and DR-linked (DC/MB) determinants, respectively. Although most non-T acute leukemias and leukemic cell lines expressed the monomorphic DR determinant detected by DA2, fewer than expected expressed the DR-linked polymorphic specificity detected by Genox 353. TdT+ lymphoid precursors from normal bone marrow were also DA2+ but Genox 353-. T cells and thymocytes which were DA2-, Genox 353- became DA2+, Genox 353+ after activation in vitro. Immunoprecipitation using DA2 and Genox 353 gave bands on polyacrylamide gel-electrophoresis which were of different molecular weights. In addition, DA2 could absorb out Genox 353 determinants from a cell lysate whereas Genox 353 could not absorb out DA2 determinants. It is concluded that DA2 and Genox 353 detect HLA-DR and DR-linked (DC1/MB1) determinants, respectively, and that these are differentially expressed on hemopoietic cells during differentiation.

Induction of HLA-DC/DS (LEU 10) antigen expression by human precursor B cell lines.

“…It is of interest to note that the DC/DS determinant detected by Genox 3.53 is not expressed on AML blasts and precursor cells of the erythroid and myeloid series (4,5). In addition, Genox 3.53 fails to stain TdT + bone marrow cells and only 20% of the pre-B cells are positive (6). It appears that DC/ DS expression is limited to more mature stages of B cell differentiation.…”

“…The recently described HLA-linked DC/DS antigens (2, 3) have more limited cell distribution (4)(5)(6) and are expressed predominantly on B cells, activated T cells, and a minor population ofmonocytes. Although their expression is documented in cases of B cell chronic lymphocytic leukemia (CLL), they are infrequently found on cells of acute lymphoblastic leukemia (ALL) of non-T cell type (4).…”

“…Although their expression is documented in cases of B cell chronic lymphocytic leukemia (CLL), they are infrequently found on cells of acute lymphoblastic leukemia (ALL) of non-T cell type (4). l Cell lines of ALL origin also fail to express these antigens (6). In addition, nonlymphoid leukemia cells as well as precursor cells of the erythroid and myeloid series have been shown to lack them (5,6).…”

“…l Cell lines of ALL origin also fail to express these antigens (6). In addition, nonlymphoid leukemia cells as well as precursor cells of the erythroid and myeloid series have been shown to lack them (5,6).…”

Expression of MHC class II antigens in human B-cell leukaemia and non-Hodgkin's lymphoma

“…Non-coordinate expression of DR and DQ antigens is also a feature of a number of established lines derived from cases of ALL Newman et al, 1983;Wang et al, 1983). That is, the majority of cells from the lines are DRpositive and fewer cells are DQ-positive.…”