Differential expression of hexose-regulated fungal genes within Amanita muscaria/Populus tremula × tremuloides ectomycorrhizas

Nehls, Uwe; Bock, Andrea; Hampp, Rüdiger

doi:10.1007/0-306-47624-x_315

Cited by 8 publications

(30 citation statements)

References 9 publications

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“…muscaria ectomycorrhizas (Nehls et al 2001). All PIP proteins contained six hydrophobic membrane-spanning domains, two conserved NPA motifs as well as accompanying amino acids supporting the selectivity for water transport.…”

Section: Discussionmentioning

confidence: 99%

“…Isolation of cDNAs containing the entire reading frame of poplar aquaporins cDNAs of all clones were obtained from an EST-project of fully developed Populus tremula x tremuloides/Amanita muscaria ectomycorrhizas (Nehls et al 2001). The missing 5¢-end of PttPIP2.2 was obtained by RACE PCR, using the SMART RACE cDNA Amplification Kit (Clontech, Palo Alto, USA) and first strand cDNA obtained from P. tremula x tremuloides/A.…”

Section: Biological Materialsmentioning

confidence: 99%

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Aquaporins in poplar: What a difference a symbiont makes!

Marjanović

Uehlein

Kaldenhoff

et al. 2005

Planta

137

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The formation of ectomycorrhizas, a tight association between fine roots of trees and certain soil fungi, improves plant nutrition in a nutrient-limited environment and may increase plant survival under water stress conditions. To investigate the impact of mycorrhiza formation on plant water uptake, seven genes coding for putative water channel proteins (aquaporins) were isolated from a poplar ectomycorrhizal cDNA library. Four out of the seven genes were preferentially expressed in roots. Mycorrhiza formation resulted in an increased transcript level for three of these genes, two of which are the most prominently expressed aquaporins in roots. When expressed in Xenopus laevis oocytes, the corresponding proteins of both genes were able to transport water. Together, these data indicate, that the water transport capacity of the plasma membrane of root cells is strongly increased in mycorrhized plants. Measurements of the hydraulic conductance of intact root systems revealed an increased water transport capacity of mycorrhized poplar roots. These data, however, also indicate that changes in the properties of the plasma membrane as well as those of the apoplast are responsible for the increased root hydraulic conductance in ectomycorrhizal symbiosis.

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Section: Discussionmentioning

confidence: 99%

Section: Biological Materialsmentioning

confidence: 99%

Aquaporins in poplar: What a difference a symbiont makes!

Marjanović

Uehlein

Kaldenhoff

et al. 2005

Planta

137

View full text Add to dashboard Cite

show abstract

“…In A. muscaria, hexose transporter gene expression is up-regulated by a threshold response mechanism, which depends on the extracellular monosaccharide concentration (Nehls et al, 1998(Nehls et al, , 2001b. In hyphae grown in the presence of hexose concentrations below 2 mM, AmMst1 is expressed at a basal level, while higher concentrations trigger an at least sixfold increase in transcript levels.…”

Section: Monosaccharide Uptake By Fungal Hyphaementioning

confidence: 99%

Sugar for my honey: Carbohydrate partitioning in ectomycorrhizal symbiosis

et al. 2007

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“…In functional ectomycorrhizas, elevated hexose transporter gene expression was exclusively observed in hyphae of the Hartig net (Nehls et al 2001a) . Differences in the apoplastic hexose concentration at the Hartig net versus the fungal sheath could be a signal that regulates fungal physiological heterogeneity in ectomycorrhizas (Nehls et al 2001b ;Nehls 2004) .…”

Section: Impact Of Nutrient Levels and Transport In Plant-fungus Commmentioning

confidence: 98%

“…In an axenic Ammanita muscaria culture, the expression of sugar importer genes is regulated by a threshold response mechanism that is dependent on the extracellular monosaccharide concentration (Nehls et al 2001a) . In functional ectomycorrhizas, elevated hexose transporter gene expression was exclusively observed in hyphae of the Hartig net (Nehls et al 2001a) .…”

Section: Impact Of Nutrient Levels and Transport In Plant-fungus Commmentioning

confidence: 99%

Communication and Signaling in the Plant–Fungus Symbiosis: The Mycorrhiza

Seddas¹,

Gianinazzi‐Pearson²,

Schoefs³

et al. 2009

Plant-Environment Interactions

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The study of symbiotic mycorrhizal associations is of fundamental and practical interest, raising questions about not only interorganism coevolution but also the ecological significance of the symbiosis in sustainable plant production systems. The partners in these associations belong to the Basidiomycota, Ascomycota or Glomeromycota, and about 95% of extant land plants. Successful colonization of roots by mycorrhizal fungi and subsequent effects on plant processes depend on recognition processes resulting from coordinated genetic programs in both partners and must be driven, at each stage, by reciprocal signaling events. This chapter summarizes current knowledge on communication and signaling in the two most frequent mycorrhizal associations: arbuscular mycorrhiza and ectomycorrhiza.

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Differential expression of hexose-regulated fungal genes within Amanita muscaria/Populus tremula × tremuloides ectomycorrhizas

Cited by 8 publications

References 9 publications

Aquaporins in poplar: What a difference a symbiont makes!

Aquaporins in poplar: What a difference a symbiont makes!

Sugar for my honey: Carbohydrate partitioning in ectomycorrhizal symbiosis

Communication and Signaling in the Plant–Fungus Symbiosis: The Mycorrhiza

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