Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickens

Abstract

The present study was aimed at investigating the role of in ovo administered amino acids: lysine, arginine, threonine or methionine plus cysteine (Met+Cys) in 14-day embryos on expression profile of growth (chicken growth hormone (cGH), insulin like growth factors (IGF) I and II, and mucin) and immunity related genes (IL-2, IL-4, . On incubation day (ID) 18, higher (P < 0.01) cGH and mucin gene expression was observed in lysine, threonine, arginine or Met+Cys injected embryos, while IGF-II expression was higher in threonine, arginine or Met+Cys injected embryos on ID 20. Expression of growth genes was down regulated (P < 0.01) on day of hatch in most of the amino acids injected chicks. On day 7 post-hatch (PH), threonine or arginine exhibited higher expression of cGH, IGF-I, and IGF-II but higher mucin gene expression only on day 14 PH. Threonine or Met+Cys injected birds had higher expression of IL-6 and TNF-α, while arginine injected birds had higher TNF-α expression. Lysine, threonine or Met+Cys injected birds had higher IL-2, but lower of IL-12 and IFN-γ gene expression. It is concluded that arginine and threonine enhanced the expression of growth related genes, while threonine and Met+Cys modulated expression of immune genes in broiler chickens.

Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens

“…() showed the enhanced FI of in ovo Thr‐administered group during days 14–21 of age. Furthermore, Arg, Thr and methionine + cysteine in ovo inoculation was found to increase the mucin gene expression in broiler chicks which may contribute to GIT development and subsequently higher FI (Kadam et al., ; Bhanja et al., ). Saki et al.…”

Effect of mannan-oligosaccharides (MOS) supplementation on performance, immunity and HSP70 gene expression in broiler chicken during hot-dry summer

“…The total RNA isolation from tissue and cDNA synthesis was carried out as per the procedure of Bhanja et al (2014). The Oligonucleotide sequences of the primers used for the gene expression study are presented in Table 2.…”

Delayed post-hatch feeding affects the performance and immunocompetence differently in male and female broiler chickens

“…The weight of bursa, spleen and thymus were recorded and expressed as per cent of live weight. Approximately 150 mg of spleen tissue was collected from each bird and total RNA isolation and cDNA synthesis were carried out as per the procedure of Bhanja et al (2014). The oligonucleotide sequences of the primers used for the gene expression study are presented in Table 1.…”

“…The oligonucleotide sequences of the primers used for the gene expression study are presented in Table 1. The expression of immune-related gene encoding IL-6 (interleukin 6), TLR-2 (toll-like receptor-2) and TNF-α (tumour necrosis factor) was analysed by real-time PCR, using an iQ5 cycler following a previously described procedure (Bhanja et al 2014).…”