Differential expression of genes involved in steroidogenesis pathway in human oocytes obtained from patients with polycystic ovaries

Al-Omar, Z.; Ozbakir, Burcu; Tulay, Pınar

doi:10.1016/j.jri.2020.103191

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…Hyperandrogenism is one of the most prominent clinical features of patients with PCOS [20,21]. Elevated expression of Cyp17A1 has been reported in the GCs, TCs, and even preovulatory oocytes of PCOS patients [20,21,31], suggesting that gene alterations in GCs and oocytes are responsible for the poor oocyte quality and decreased fertilization rates in IVF patients with PCOS [32]. Furthermore, Klf4 expression is down-regulated in the ovaries of PCOS patients [20].…”

Section: Discussionmentioning

confidence: 99%

Luteinizing Hormone Surge-Induced Krüppel-like Factor 4 Inhibits Cyp17A1 Expression in Preovulatory Granulosa Cells

Choi,

Lee,

Ryu

et al. 2023

Biomedicines

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Previous in vivo and in vitro studies have demonstrated a dramatic up-regulation of Krüppel-like factor 4 (Klf4) in rat preovulatory granulosa cells (GCs) after LH/hCG treatment and its role in regulating Cyp19A1 expression during the luteal shift in steroidogenesis. In this study, we examined whether Klf4 also mediates the LH-induced repression of Cyp17A1 expression in primary rat preovulatory GCs. In response to LH treatment of GCs in vitro, Cyp17A1 expression declined to less than half of its initial value by 1 h, remaining low for 24 h of culture. Overexpression of Klf4 decreased basal and Sf1-induced Cyp17A1 expressions and increased progesterone secretion. Reduction of endogenous Klf4 by siRNA elevated basal Cyp17A1 expression but did not affect LH-stimulated progesterone production. Overexpression of Klf4 also significantly attenuated Sf1-induced Cyp17A1 promoter activity. On the other hand, mutation of the conserved Sp1/Klf binding motif in the promoter revealed that this motif is not required for Klf4-mediated repression. Taken together, these data indicate that the Cyp17A1 gene may be one of the downstream targets of Klf4, which is induced by LH in preovulatory GCs. This information may help in identifying potential targets for preventing the molecular changes occurring in hyperandrogenic disorders.

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Section: Discussionmentioning

confidence: 99%

Luteinizing Hormone Surge-Induced Krüppel-like Factor 4 Inhibits Cyp17A1 Expression in Preovulatory Granulosa Cells

Choi,

Lee,

Ryu

et al. 2023

Biomedicines

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“…Patients with polycystic ovaries showed slightly greater follicle and oocyte numbers compared to the control group. HSD17B1 expression in oocytes was statistically different, indicating that polycystic ovaries had changed steroidogenesis pathways, potentially playing a role in their development (Al-Omar et al, 2020).…”

Section: Correlation Between Dna Methylation and Mrna Expression Of T...mentioning

confidence: 95%

“…According to Al-Omar et al (2020), the oocytes' HSD17B1 expression level varied significantly (p 0.05).…”

Section: Correlation Between Dna Methylation and Mrna Expression Of T...mentioning

confidence: 99%

Associations of HSD17B1 Gene Expression with its DNA Methylation and Estradiol Level in Polycystic Ovary Syndrome Indonesian Patients

Puspita,

Hestiantoro,

Hestiantoro

et al. 2024

OnLine Journal of Biological Sciences

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expression in PCOS patients is not significant. (p = 0.076). A significant negative association has been seen between the mRNA. There is a significant negative correlation between the mRNA expression of the HSD17B1 gene and serum E2 levels. (p = 0.020). "The more down-regulated mRNA expression of the HSD17B1 gene, the lower serum E2 levels." The integrated analysis in this study was hypomethylated DNA and down-regulated mRNA expression of HSD17B1 genes. The hypomethylated DNA was not involved in down-regulating mRNA expression. Therefore, down-regulated mRNA expression of the HSD17B1 gene in PCOS patients can cause lower E2 levels in PCOS, preventing cell growth and potentially contributing to the cause of PCOS pathogenesis.

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Expression of genes in the AKT signalling pathway in human oocytes from patients with polycystic ovaries

Hafizi

Ozbakir

Tulay

2023

Zygote

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Summary Polycystic ovary syndrome is an endocrine disorder commonly found among females of reproductive age. Different factors have been correlated with this syndrome, although the aetiology of the disease is still unrecognized with both environmental and hereditary factors leading to the progression. Hormonal effects of the AKT pathway have made it an interesting study unit for PCOS cases. The aim of this study was to investigate the expression patterns of genes involved in the AKT pathway, including IRS1, IRS2, AKT1 and AKT2. In total, 13 human oocytes were collected for this study at the meiosis II stage, in which seven of them were collected from individuals with polycystic ovaries and the rest formed the control group of individuals with no signs of polycystic ovaries. RNA was extracted from oocytes and then the RNA was converted into cDNA for the real-time PCR process. Expression levels of four genes in the AKT pathway, in addition to housekeeping gene (ACTB), were evaluated. Expression levels of each gene were quantified using real-time PCR and statistical analysis was performed. The results of this study showed that there was no significant correlation between the expression of genes in oocyte samples obtained from patients with polycystic ovaries and the control group. This study is the first to evaluate the expression levels of genes involved in the AKT pathway in human oocyte samples. Therefore, it provides crucial information to form the basis of further studies.

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Differential expression of genes involved in steroidogenesis pathway in human oocytes obtained from patients with polycystic ovaries

Cited by 7 publications

References 28 publications

Luteinizing Hormone Surge-Induced Krüppel-like Factor 4 Inhibits Cyp17A1 Expression in Preovulatory Granulosa Cells

Luteinizing Hormone Surge-Induced Krüppel-like Factor 4 Inhibits Cyp17A1 Expression in Preovulatory Granulosa Cells

Associations of HSD17B1 Gene Expression with its DNA Methylation and Estradiol Level in Polycystic Ovary Syndrome Indonesian Patients

Expression of genes in the AKT signalling pathway in human oocytes from patients with polycystic ovaries

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