Differential expression of Fas system apoptotic molecules in peripheral lymphocytes from patients with Graves' disease and Hashimoto's thyroiditis.

Fountoulakis, Stelios; Vartholomatos, George; Kolaitis, Nikolaos; Frillingos, Stathis; Philippou, George; Tsatsoulis, Agathocles

doi:10.1530/eje-08-0092

Cited by 29 publications

(34 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported previously, Th1 and Th2 cells are the extreme polarized forms of CD4 + Th cells, when it becomes dangerous, Th1 can be shifted to a less polarized profile Th0, or even Th2, via a process called immune deviation (29). Predominance of the immune response of Th1 accelerates the apoptosis of thyrocytes (30), which mediated by Fas and TRAIL, leading to HT (2,31). Whereas the predominance of a Th2 immune response induces antigen-specific B cells to produce antithyroid antibodies, just as stimulatory antiTSH receptor antibodies are responsible for Graves' disease and the blocking antiTSH receptor antibodies are responsible for atrophic thyroiditis; thus, the balance of Th1-Th2 directly affects the clinical expression of thyroid autoimmunity (32).…”

Section: Discussionmentioning

confidence: 99%

Immunological changes of T helper cells in flow cytometer‑sorted CD4+ T cells from patients with Hashimoto's thyroiditis

et al. 2018

View full text Add to dashboard Cite

Abstract. Recent incidence rates for Hashimoto's thyroiditis (HT) and hypothyroidism are higher than those of previous studies. Previous studies indicated that T helper cells may have a major role in the pathogenesis and development of HT, but there is no consensus in the literature. The aim of the present study was to explore the peripheral T helper cell response in the different stages of HT. In this cross-sectional study, we performed flow cytometry analysis to determine the various T cell subsets of 389 patients with HT (34 patients with HT who developed overt hypothyroidism, and 148 patients with HT who developed subclinical hypothyroidism), as well as 51 healthy controls. Anti-thyroid antibodies, and thyroid function were measured. The findings demonstrated that the proportion of peripheral Th1 cells was significantly lower in patients with HT than in healthy euthyroid controls (P<0.001), and the proportion of peripheral Th2, Treg cells was significantly higher in patients with HT than in healthy euthyroid controls (P<0.001). Therefore the Th1/Th2 ratio was significantly lower in HT patients than in healthy euthyroid controls (P<0.001). The Th17/Treg ratio in HT patients was significantly lower than that control subjects (P<0.001). Th1 proportions in patients with overt hypothyroidism HT were significantly higher than in subclinical hypothyroidism HT patients (P=0.031). In conclusion, the findings of the present study demonstrated that there is an increased immune deviation of Th1 lymphocytes and compensatory accelerating activity of Treg cells in HT, and the peripheral Th1 cells from the HT patients correlated to the developmental stage of hypothyroidism.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunological changes of T helper cells in flow cytometer‑sorted CD4+ T cells from patients with Hashimoto's thyroiditis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Scant data are available in the medical literature on the serum levels of sFas and sFasL in hypothyroidism [23,30,31]. However, serum levels of sFas were investigated in patients with autoimmune thyroid diseases (AITD) [23,30,32].…”

Section: Discussionmentioning

confidence: 99%

Influence of levothyroxine treatment on serum levels of soluble Fas (CD95) and Fas Ligand (CD95L) in chronic autoimmune hypothyroidism

Nabipour

Kalantarhormozi

Assadi

et al. 2010

Endocr

View full text Add to dashboard Cite

Fas/FasL-mediated apoptosis results in the destruction of thyrocytes in chronic autoimmune hypothyroidism (CAIH). In this study, we examined the serum levels of soluble Fas (sFas) and soluble sFas ligand (sFasL) in euthyroid patients with chronic autoimmune hypothyroidism, who were taking levothyroxine (euthyroid, LT4-CAIH), to investigate the possible role of thyroid hormone therapy in down-regulation of apoptotic factors. Fifty euthyroid patients with CAIH on levothyroxine (median of duration 36 months, range 6-228 months) were compared with 75 age- and sex-matched healthy individuals. Serum levels of soluble Fas and soluble Fas Ligand, autoantibodies to thyroid peroxide and thyroglobulin were measured using ELISA. Serum levels of sFas were significantly higher in the euthyroid, LT4-CAIH group [median 9.12 ng/ml, interquartile range (7.86-10.72 ng/ml)] than in the controls [6.11 ng/ml (5.60-6.81 ng/ml)] (P < 0.0001). Compared with controls [80.33 pg/ml (68.22-103.70 pg/ml)], the euthyroid, LT4-CAIH group [125.71 pg/ml (106.11-149.48 pg/ml)] had significantly higher levels of sFasL (P < 0.0001). In a chronological study, there was no significant correlation between sFas, sFasL, and the duration of levothyroxine therapy. In conclusion, normalization of serum sFas and sFasL levels cannot be achieved during levothyroxine treatment in patients with CAIH. It appears that levothyroxine therapy has no important effect on down-regulation of apoptotic factors in CAIH. Thus, like thyroid autoantibodies, monitoring of serum levels of sFas/sFasL is not indicated during thyroid hormone therapy.

show abstract

“…Therefore, the Fas pathway is the most important mechanism of Tlymphocyte mediated apoptosis. It is just possible that this process plays an essential role in the pathogenesis of Hashimoto thyroiditis, because cytotoxic T lymphocytes are fully present in the thyroid in places where apoptosis is located (Mitsiades et al ., 1998 ;Fountoulakis et al, 2008;Chen el al ., 2004;Baker., 1999;Bretz.,2002).…”

Section: Apoptosis In Htmentioning

confidence: 99%

Hashimoto's Disease

Bougacha-Elleuch¹,

Mnif-Feki²,

Charfi-Sellami³

et al. 2012

A New Look at Hypothyroidism

View full text Add to dashboard Cite

Differential expression of Fas system apoptotic molecules in peripheral lymphocytes from patients with Graves' disease and Hashimoto's thyroiditis.

Abstract: The Fas system apoptotic molecules appear to be differentially expressed on peripheral lymphocytes in the two opposite phenotypes of AITD.

Cited by 29 publications

References 36 publications

Immunological changes of T helper cells in flow cytometer‑sorted CD4+ T cells from patients with Hashimoto's thyroiditis

Immunological changes of T helper cells in flow cytometer‑sorted CD4+ T cells from patients with Hashimoto's thyroiditis

Influence of levothyroxine treatment on serum levels of soluble Fas (CD95) and Fas Ligand (CD95L) in chronic autoimmune hypothyroidism

Hashimoto's Disease

Contact Info

Product

Resources

About