Differential expression of defense-related genes in chilli pepper infected with anthracnose pathogen Colletotrichum truncatum

Mishra, Rukmini; Nanda, Satyabrata; Rout, Ellojita; Chand, Subodh Kumar; Mohanty, Jatindra Nath; Joshi, Raj Kumar

doi:10.1016/j.pmpp.2016.11.001

Cited by 32 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pepper is an important cash crop with high nutritional and medicinal value (Sundaramoorthy et al, 2012). It contains various forms of vitamins (such as vitamin A, B, C, E, K), mineral substances, dietary fibers, and natural pigments that are good for human health (Mishra et al, 2017). However, the growth and production of pepper are affected by indigenous pathogens resulting in pepper yield reduction.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.)

Huang

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Pepper seedling wilt disease is the main cause of crop yield reduction. Biocontrol agents are widely used to control plant diseases caused by pathogenic fungi and activate plant defense systems. Our preliminary work showed that Bacillus subtilis SL-44 played a significant role in the reduction of wilt disease severity on pepper plants. To evaluate biological control mechanism of B. subtilis SL-44 on wilt disease caused by Rhizoctonia solani, the activities of the related enzymes were detected in the pepper seedling with different treatment in this study. Fluorescence microscopy combined with different dyes showed that B. subtilis SL-44 induced a large amount of active oxygen and callose accumulation in pepper leaves. The defense-related enzyme activities in pepper were improved significantly when treated with B. subtilis SL-44, including peroxidase, catalase, superoxide dismutase, polyphenol oxidase, and phenylalanine ammonia lyase. The activity of chitinase and β-1,3-glucanase in B. subtilis SL-44-treated pepper was also enhanced. Furthermore, the expression level of pepper-resistance gene CaPIN II was significantly increased in B. subtilis SL-44 treatment. Besides, B. subtilis SL-44 filtrate led to the death of the pathogenic fungus by fracturing the mycelia and leaking of the cell contents. Surfactin, iturin, and fengycin were found in B. subtilis SL-44 crude extracts, which could be effective antifungal compounds against R. solani. The results suggest that B. subtilis SL-44 could not only activate induced systemic resistance of pepper seedling against wilt disease caused by R. solani by jasmonic acid-dependent signaling pathway but also produce antifungal compounds to inhibit or even damage the mycelium growth of R. solani. The findings of this study provide novel guidance in plant protection development.

show abstract

Section: Introductionmentioning

confidence: 99%

Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.)

Huang

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Salicylic acid (SA) is associated with resistance to biotrophs and hemibiotrophs, while jasmonic acid (JA) and ethylene (ET) regulate defense during necrotrophic infection 46,47 . The transcript accumulation of JA and ET responsive genes such as plant defensin 1.2 (PDF1.2), Lypoxygenase 3 (Lox3), Allene oxide synthase (AOS), ACC synthase 2 (ACS2), phenylalanine ammonia-lyase 3 (PAL3), and pathogenesis related proteins (PR2 and PR5) were more rapid and had higher induction in the resistant cultivar of chili and C. truncatum pathosystem 15 . These genes, related to pepper defense response against fungal interaction, were observed in this study, and were also rapidly induced in ripe fruits, while in unripe fruits the response was delayed.…”

Section: Discussionmentioning

confidence: 99%

“…However, the specific effects of anthracnose interaction on transcriptome-level responses are still poorly understood. While studies of individual expression of defense-related genes have provided insights into pepper responses to anthracnose 14 , 15 , large scale transcriptome studies allow for contrasts of whole expression profiles. These comparisons are an interesting subject for pepper breeding purposes, since anthracnose is caused mainly by C. scovillei and can affect unripe and ripe fruits stages, although red pepper fruits seem to be more resistant than green fruits 16 , 17 .…”

Section: Introductionmentioning

confidence: 99%

Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages

Baba

Powell

Ivamoto

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3′ RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (Uf) and ripe fruits (Rf) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epiaristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs. Chili peppers originate from the Americas and they are among the oldest cultivated plants, dating from before 3400 BC. Capsicum annuum L. is the most commercially important species, due to its usefulness in the human diet, medicines, beverages, and as ornamentals. Pepper fruits have several nutraceutical benefits for human health, due to a variety of antioxidant, anti-inflammatory, antimicrobial, anti-carcinogenic, and cardio-protective properties 1. Anthracnose is caused by Colletotrichum spp., and represents the major disease of chili fruit worldwide, leading to significant postharvest yield loss and reducing marketability 2. Twenty-four Colletotrichum species have been identified as pathogens of chili anthracnose, with the three main pathogenic species being C. scovillei (previously identified as C. acutatum), C. truncatum (syn. C. capsici), and C. siamense (previously identified as C. gloeosporioides) 3 .

show abstract

“…Genomic and its Coding sequences (CDS) of deduced RLK hot pepper proteins were retrieved from Sol Genomics Network (https://solgenomics.net/). Primer sets for RT-qPCR were designed in 3 and 5 untranslated regions of individual genes to avoid non-specific amplification using Prime Quest Tool (http://eu.idtdna.com/PrimerQuest/Home/Index) [23]. For all primers Ubiquitin 3 was used as reference gene.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Analysis of Non-Arginine Aspartate Receptor like kinase gene family under Colletotrichum truncatum stress conditions in Hot pepper

Srideepthi

Krishna

Suneetha

et al. 2020

Preprint

View full text Add to dashboard Cite

Receptor Like kinases (RLKs) are conserved upstream signaling molecules that regulate several biological processes, including plant development and stress adaptation. Non arginine aspartate (non-RD) an important class of RLKs plays a vital role in disease resistance and apoptosis in plants. In present investigation, a comprehensive Insilco analysis for non-RD Kinase gene family including identification, sequence similarity, phylogeny, chromosomal localization, gene structures, gene duplication analysis, promoter analysis and transcript expression profiles were elucidated. In this study twenty six genes were observed on nine out of twelve chromosomes. All these genes were clustered into seven subfamilies under large monophyletic group termed as Interleukin-1 Receptor-Associated Kinase (IRAK) family. Structural diversity in genomic structure among non-RD kinase gene family were identified and presence of pathogen induced cis regulatory elements like STRE, MYC, MYB,W box were found. Expression profiles of genes involved in providing resistance to anthracnose pathogen Colletotrichum truncatum in hot pepper were analyzed at different infective stages in both resistant and susceptible genotypes. Among twenty six genes, CaRLK1 gene belonging to LRRXII subfamily was up regulated under severe stress after infection in resistant genotype PBC-80. This integrative approach has helped us to identify candidate genes involved in disease resistance which would be helpful in future crop improvement programs.

show abstract

Differential expression of defense-related genes in chilli pepper infected with anthracnose pathogen Colletotrichum truncatum

Cited by 32 publications

References 50 publications

Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.)

Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.)

Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages

Genome-Wide Identification, Characterization and Expression Analysis of Non-Arginine Aspartate Receptor like kinase gene family under Colletotrichum truncatum stress conditions in Hot pepper

Contact Info

Product

Resources

About