Differential Expression of Cytochrome P450 CYP6 Genes in the Brown Marmorated Stink Bug, Halyomorpha halys (Hemiptera: Pentatomidae)

Mittapelly, Priyanka; Bansal, Raman; Michel, Andy

doi:10.1093/jee/toz007

Cited by 16 publications

(13 citation statements)

References 60 publications

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Section: Discussionmentioning

confidence: 99%

“…Notably, N. lecontei larvae frequently ingest pine bark in addition to pine needles (Wilson 1992), suggesting that CYP3 may expand predictably in wood feeders. Additionally, one of the two Neodiprion-specific CYP3 clades with evidence of positive selection (Table 3) belongs to the CYP6 subfamily, which is linked to host plant adaptation in several insect taxa (Li et al 2003;Li et al 2007;Feyereisen 2012;Mittapelly et al 2019). Because pine resin has antimicrobial (Himejima et al 1992;Cowan 1999;Gershenzon and Dudareva 2007) and fungicidal properties (Grayer and Harborne 1994), we hypothesized that N. lecontei co-opted these compounds for its own defense, leading to relaxed selection on genes involved in immunity and a reduced innate immune response.…”

Section: Within-genome Signatures Of Gene-family Size Changementioning

confidence: 99%

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Ecological correlates of gene family size in a pine-feeding sawfly genome and across Hymenoptera

Vertacnik

Herrig

Godfrey

et al. 2021

Preprint

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A central goal in evolutionary biology is to determine the predictability of adaptive genetic changes. Despite many documented cases of convergent evolution at individual loci, little is known about the repeatability of gene family expansions and contractions. To address this void, we examined gene family evolution in the redheaded pine sawfly Neodiprion lecontei, a non-eusocial hymenopteran and exemplar of a pine-specialized lineage evolved from angiosperm-feeding ancestors. After assembling and annotating a draft genome, we manually annotated multiple gene families with chemosensory, detoxification, or immunity functions and characterized their genomic distributions and evolutionary history. Our results suggest that expansions of bitter gustatory receptor (GR), clan 3 cytochrome P450 (CYP3), and antimicrobial peptide (AMP) subfamilies may have contributed to pine adaptation. By contrast, there was no evidence of recent gene family contraction via pseudogenization. Next, we compared the number of genes in these same families across insect taxa that vary in diet, dietary specialization, and social behavior. In Hymenoptera, herbivory was associated with large GR and small olfactory receptor (OR) families, eusociality was associated with large OR and small AMP families, and--unlike investigations among more closely related taxa--ecological specialization was not related to gene family size. Overall, our results suggest that gene families that mediate ecological interactions may expand and contract predictably in response to particular selection pressures, however, the ecological drivers and temporal pace of gene gain and loss likely varies considerably across gene families.

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Section: Discussionmentioning

confidence: 99%

Section: Within-genome Signatures Of Gene-family Size Changementioning

confidence: 99%

Ecological correlates of gene family size in a pine-feeding sawfly genome and across Hymenoptera

Vertacnik

Herrig

Godfrey

et al. 2021

Preprint

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“…In the pea aphid Acyrthosiphon pisum, changes in transcription were more pronounced for race effect than host plant effect, with only a few hundred genes having expression changes attributable to diet type (Eyres et al, 2016). More recently, in the closely related stink bug Halyomorpha halys, no significant changes were detected on different host plants for cytochrome P450 enzymes involved in detoxification (Mittapelly et al, 2019). Our results of a somewhat moderate response in transcriptional changes after feeding on different diets are therefore not entirely unexpected and are similar to those observed for other hemipterans, in contrast to clear responses reported for lepidopteran and coleopteran species.…”

Section: Discussionmentioning

confidence: 99%

Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets

Cantón

Bonning

2020

Front. Physiol.

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Nezara viridula is a polyphagous stink bug that feeds on crops of economic importance such as corn, soybean and cotton. To increase understanding of the ability of this pest insect to feed on such diverse cropping systems, we analyzed the impact of an exclusive diet of corn or green bean on the enzymatic activity and transcriptomic profile of digestive enzymes. Growth rate and survival were reduced when insects were reared exclusively on green bean compared to corn. However, the overall protease and nuclease activity profiles were comparable between the two treatments. Distinct differences in inhibitor sensitivity and activity were seen in some cases, particularly for serine proteases in some regions of the midgut. The transcription profiles from N. viridula fed on corn versus green bean were distinct on principal component analysis of RNA-seq data. While specific transcripts differentially transcribed according to diet and across several tissues were identified, a large number of these transcripts remain unannotated. Further annotation for identification of these genes will be important for improved understanding of the remarkable polyphagy of N. viridula.

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“…P450s are particularly well studied; upregulation of P450s in the midgut has often been found to underpin insecticide resistance [18][19][20]. Information on P450s in stink bugs is currently limited to one species Halyomorpha halys, where a preliminary identification and analysis has been performed [21,22].…”

Section: Introductionmentioning

confidence: 99%

A transcriptomic and proteomic atlas of expression in the Nezara viridula (Heteroptera: Pentatomidae) midgut suggests the compartmentalization of xenobiotic metabolism and nutrient digestion

et al. 2020

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Background: Stink bugs are an emerging threat to crop security in many parts of the globe, but there are few genetic resources available to study their physiology at a molecular level. This is especially true for tissues such as the midgut, which forms the barrier between ingested material and the inside of the body. Results: Here, we focus on the midgut of the southern green stink bug Nezara viridula and use both transcriptomic and proteomic approaches to create an atlas of expression along the four compartments of the anterior-posterior axis. Estimates of the transcriptome completeness were high, which led us to compare our predicted gene set to other related stink bugs and Hemiptera, finding a high number of species-specific genes in N. viridula. To understand midgut function, gene ontology and gene family enrichment analyses were performed for the most highly expressed and specific genes in each midgut compartment. These data suggested a role for the anterior midgut (regions M1-M3) in digestion and xenobiotic metabolism, while the most posterior compartment (M4) was enriched in transmembrane proteins. A more detailed characterization of these findings was undertaken by identifying individual members of the cytochrome P450 superfamily and nutrient transporters thought to absorb amino acids or sugars. Conclusions: These findings represent an initial step to understand the compartmentalization and physiology of the N. viridula midgut at a genetic level. Future studies will be able to build on this work and explore the molecular physiology of the stink bug midgut.

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Differential Expression of Cytochrome P450 CYP6 Genes in the Brown Marmorated Stink Bug, Halyomorpha halys (Hemiptera: Pentatomidae)

Cited by 16 publications

References 60 publications

Ecological correlates of gene family size in a pine-feeding sawfly genome and across Hymenoptera

Ecological correlates of gene family size in a pine-feeding sawfly genome and across Hymenoptera

Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets

A transcriptomic and proteomic atlas of expression in the Nezara viridula (Heteroptera: Pentatomidae) midgut suggests the compartmentalization of xenobiotic metabolism and nutrient digestion

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