Differential expression of circulating Th1/ Th2/ Th17 cytokines in serum of Chlamydia trachomatis-infected women undergoing incomplete spontaneous abortion

Prasad, Priya; Singh, Namita; Das, Banashree; Raisuddin, Sheikh; Dudeja, Mridu; Rastogi, Sangita

doi:10.1016/j.micpath.2017.06.031

Cited by 15 publications

(21 citation statements)

References 42 publications

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“…To cause an in ammatory reaction, pathogenic agents need access the intrauterine surroundings of the fetal tissues or endometrium, which can result in miscarriage [3,39]. Prasad et al investigated the role of Th1/Th2/Th17 cytokines in the immunopathogenesis process of miscarriage in Chlamydia trachomatis (Ct)-positive rst-trimester aborters [19,40]. They found that some cytokines such as IL-6, IFN-γ, IL-2, TNF-α, and IL-17A were noticeably increased in spontaneous abortion (SA) group and recurrent miscarriage(RM) group (Ct-infected) versus controls.…”

Section: Discussionmentioning

confidence: 99%

Molecular Investigation of Association Among Common IL-6 Polymorphism with Cytomegalovirus(CMV) Infection and Recurrent Miscarriage in Iranian Women

Ardakani

Rahimi

Panahi

et al. 2021

Preprint

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Background: Recurrent pregnancy loss (RPL) is described as two or more spontaneous abortions. Until now, although various factors such as genetic, endocrinology, anatomy, immunology, and microbiology have been distinguished that affect abortions, the precise basic etiology in up to 50% of RPL cases are not determined. Human cytomegalovirus (CMV) infection and host genetic background, like IL-6 SNP polymorphisms play important roles in RPL etiology. Objective: This study aimed to evaluate relationship among single nucleotide polymorphisms (-634C/G and -174 G/C) in the IL-6 gene with CMV infection and risk of RPL for early detection and treatment of RPL. Materials and methods: This case-control study was carried on 80 Iranian females with RPL and 80 healthy females as control group. The extraction of DNA from samples and detection of CMV and IL6 SNPs were determined by Tetra ARMS-PCR. Finally, the statistical analysis for detection CMV and two polymorphisms roles in RPL were analyzed by Epi Info TM software by X2 test. Results: Our results indicated an increased rate of CMV infection in RPL group (44%) versus the control group (25.45%). Also, the prevalence of IL-6 -634C/G genotype among RPL patients with CMV infection was 80%, while the frequency of this genotype among RPL patients without CMV infection was 50%. Furthermore, no substantial relation was found between IL-6 -174 G/C genotypes and RPL (P ≤0.0001). BesidesConclusion: This study not only indicated a significant role of CMV in RPL, but also showed CMV association with allele G in IL6 -634 among Iranian women. In addition, suggested the use of CMV and IL-6 -634 GG genotypes in RPL as diagnostic and prognostic biomarkers in Iranian population.

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Section: Discussionmentioning

confidence: 99%

Molecular Investigation of Association Among Common IL-6 Polymorphism with Cytomegalovirus(CMV) Infection and Recurrent Miscarriage in Iranian Women

Ardakani

Rahimi

Panahi

et al. 2021

Preprint

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“…Perubahan fisiologis pada wanita hamil akan berdampak pada perjalanan dan manifestasi klinis infeksi, salah satunya ineksi yang disebabkan oleh Chlamydia trachomatis. 8 Infeki C. trachomatis dalam kehamilan dapat dihubungkan dengan abortus spontan, kehamilan ektopik, kematian janin dalam kandungan, infeksi perinatal, intrauterine growth restriction, kelainan kongenital, ketuban pecah dini, prematuritas, chorioamnionitis, infeksi puerperalis dan infeksi neonatal. 9 Prevalensi infeksi C. trachomatis pada wanita hamil di iran tercatat adalah 18,2%, sedangkan di brazil prevalensi infeksi C. trachomatis pada wanita hamil adalah 11,0%.…”

Section: Pendahuluanunclassified

“…Infeksi Chlamydia sebagai suatu penyakit menular seksual (Sexually Transmitted Infection) dapat melibatkan beberapa organ, yaitu cerviks, urethra, salping, uterus dan epidydimis. 17 Dalam jangka pendek maupun jangka panjang, infeksi Chlamydia yang tidak terobati dapat menyebabkan masalah kesehatan yang serius, baik pada pria dan wanita, demikian juga pada bayi yang dilahirkan dari ibu yang telah terinfeksi. Insidensi infeksi C. trachomatis genitalis lebih sulit diperkirakan.…”

Section: Hubungan Infeksi Chlamydia Trachomatis Dengan Kejadian Abortunclassified

HUBUNGAN INFEKSI Chlamydia trachomatis DENGAN KEJADIAN ABORTUS SPONTAN DI RSUD DR. RASIDIN DAN RSIA SITI HAWA PADANG

Octarina¹,

Putra²,

Antonius³

2018

JKA

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AbstrakAbortus merupakan salah satu penyebab dari morbiditas dan mortalitas maternal. Abortus adalah berakhirnya kehamilan pada atau sebelum kehamilan tersebut berusia 22 minggu. Banyak faktor yang mempengaruhi abortus, salah satunya adalah infeksi yang disebabkan oleh Chlamydia trachomatis, selain itu faktor resiko lain yang mempengaruhi terjadinya abortus adalah usia maternal, paritas, riwayat kejadian abortus pada kehamilan sebelumnya, dan jarak kehamilan. Tujuan penelitian ini adalah untuk mengetahui hungan infeksi Chlamydia trachomatis dengan kejadian abortus spontan. Penelitian ini merupakan studi analitik dengan desain case control yaitu untuk mengetahui infeksi C. trachomatis dengan kejadian abortus spontan. Populasi penelitian adalah semua ibu hamil dengan abortus spontan di RS dr. Rasidin padang dan RSIA Siti Hawa Padang. Teknik pengambilan sampel dengan consecutive sampling yaitu setiap penderita yang memenuhi kriteria inklusi dimasukkan dalam subyek penelitian sampai jumlah sampel tercapai yaitu sebanyak 50 sampel. Hasil penelitian didapatkan Proporsi kejadian abortus pada sampel positif C. trachomatis yaitu 44,0%. Analisa statistik dengan uji Fisher's Exact Test menunjukan, terdapat hubungan yang bermakna antara infeksi C.trachomatis dengan kejadian abortus (p<0.05) dengan nilai Odds Ratio (OR) 5,7. Dari hasil penelitian dapat disimpulkan terdapat hubungan antara infeksi C.trachomatis dengan kejadian abortus. Diharapkan untuk penelitian berikutnya melakukan penelitian lebih lanjutan mengenai hubungan faktor resiko maternal dan infeksi Chlamydia trachomatis terhadap kejadian abortus spontan dengan metode penelitian yang berbeda dan jumlah sampel yang lebih banyak. AbstractAbortion is one of the causes of maternal morbidity and mortality. Abortion is the end of a pregnancy on or before the pregnancy is 22 weeks. Many factors affect the abortion, one of them is an infection caused by Chlamydia trachomatis, besides other risk factors that affect the occurrence of abortion is maternal age, parity, history of abortion in previous pregnancy, and the distance of pregnancy. The objective of this study was to know the relationship between C. trachomatis infection with abortion events. This study is an analytic study with case control design that is to know the relationship between C. trachomatis infection with abortion events. The participants were all pregnant women with spontaneous abortion in dr. Rasidin padang and RSIA Siti Hawa Padang. Technique of sampling with consecutive sampling that every patient who meets inclusion criteria included in the subject of research until the number of samples reached as many as 50 samples.The results of the study were the proportion of abortus occurrence in C. trachomatis positive sample was 44.0%. Statistical analysis with Fisher's Exact Test showed that there was a significant correlation between C. trachomatis infection with abortion (p <0.05) and Odds Ratio ( OR) 5.7. From the results of the study it can be concluded there are significant relationship between C. trach...

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“…T-cells are important for orchestrating the immune response and can be categorized into subsets according to their phenotypic characteristics resulting from polarization of naïve T-cells (i.e. Th-1, Th-2 and regulatory T-cell; T-reg) [17][18][19] . Each T-cell subset has a unique functional role highlighted by their capacity to produce pro-inflammatory and anti-inflammatory cytokines in response to various immune challenges.…”

mentioning

confidence: 99%

“…Given the critical role of cytokines in regulating immune responses, subtle differences in cytokine expression may have a major effect on the outcomes of those responses 20 . Single nucleotide polymorphisms (SNP) in the regulatory region of the cytokine genes have been shown to correlate with individual variations in cytokine production [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] . Thus, variations in susceptibility to NODAT may be influenced by inter-individual genetic variations of cytokine genes [19][20][21][22][23] .…”

mentioning

confidence: 99%

Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant

Jahromi

Al-Otaibi²,

Gheith

et al. 2021

Sci Rep

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New Onset Diabetes After Transplantation (NODAT) is a serious metabolic complication. While β-cell dysfunction is considered the main contributing factor in the development of NODAT, the precise pathogenesis is not well understood. Cytokines are thought to be involved in the inflammation of islet β-cells in diabetes; however, few studies have investigated this hypothesis in NODAT. A total of 309 kidney transplant recipients (KTRs) were included in this study. An association between kidney transplants, and the development of diabetes after transplant (NODAT) was investigated. Comparison was made between KTRs who develop diabetes (NODAT cases) or did not develop diabetes (control), using key cytokines, IL-6 G (− 174)C, macrophage mediator; IL-4 C (− 490)T, T helper (Th)-2 cytokine profile initiator; Th-1 cytokine profile initiator interferon-γ T (+ 874) A gene and TGF β1 C (+ 869) T gene polymorphisms were investigated. The genes were amplified using well-established polymerase chain reaction (PCR) techniques in our laboratory. Compared to the AA and AT genotypes of interferon gamma (IFNG), there was a strong association between the TT genotype of IFNG and NODAT kidney transplant recipients (KTRs) versus non-NODAT KTRs (p = 0.005). The AA genotype of IFNG was found to be predominant in the control group (p = 0.004). Also, significant variations of IL6 G (− 174) C, IL-4 C (− 590) T, interferon-γ T (+ 874) A gene and transforming growth factor β1 C (+ 869) T may contribute to NODAT. Our data is consistent with theTh-1/T-reg pathway of immunity. Further larger pan Arab studies are required to confirm our findings.

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Differential expression of circulating Th1/ Th2/ Th17 cytokines in serum of Chlamydia trachomatis-infected women undergoing incomplete spontaneous abortion

Cited by 15 publications

References 42 publications

Molecular Investigation of Association Among Common IL-6 Polymorphism with Cytomegalovirus(CMV) Infection and Recurrent Miscarriage in Iranian Women

Molecular Investigation of Association Among Common IL-6 Polymorphism with Cytomegalovirus(CMV) Infection and Recurrent Miscarriage in Iranian Women

HUBUNGAN INFEKSI Chlamydia trachomatis DENGAN KEJADIAN ABORTUS SPONTAN DI RSUD DR. RASIDIN DAN RSIA SITI HAWA PADANG

Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant

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