Differential expression of cDNA coding for chalcone reductase, a key enzyme of the 5-deoxyflavonoid pathway, under various stress conditions in Medicago sativa

Sallaud, Christophe; El-Turk, Joumana; Breda, C.; Buffard, Dominique; Kozak, Isabelle de; Esnault, R.; Kondorosi, Ádám

doi:10.1016/0168-9452(95)04179-x

Cited by 29 publications

(13 citation statements)

References 29 publications

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“…CHS is an enzyme of central importance in the biosynthetic pathway of all classes of flavonoids (Hahlbrock and Scheel 1989). The genes coding for the enzymes CHS, chalcone reductase, chalcone isomerase, chalcone O‐methyltransferase and IFR ( chs , chr , chi , chomt and ifr , respectively) are expressed at high levels in roots of legumes (Djordjevic et al 1997, Maxwell et al 1993, McKhann and Hirsch 1994, Paiva et al 1991, Sallaud et al 1995). The expression of chs is under complex tissue‐specific and developmental regulation.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of nodulation inMedicago truncatulaby low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Fei

Vessey

2009

Physiologia Plantarum

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Although mineral nitrogen generally has negative effects on nodulation in legume-rhizobia symbioses, low concentrations of ammonium stimulate nodulation in some legumes. In this study, the effects of ammonium and nitrate on growth, nodulation and expression of 2 nitrogen transport and 12 putative nodulation-related genes of the model symbiosis of Medicago truncatula - Sinorhizobium meliloti are investigated. After 3 weeks of hydroponic growth, whole-plant nodulation was enhanced in all the ammonium treatments and up to three-fold in the 0.5 mM treatment compared with the zero-nitrogen control. Specific nodulation (nodules g(-1) root dry weight) was greatly stimulated in the 0.1 and 0.5 mM NH4+ treatments, to a lower extent in the 0.1 mM NO3- treatment, and inhibited in all other treatments. Expression of the 14 selected genes was observed at 0, 6, 12 and 24 h after exposure to rhizobia and nitrogen. Expression of nitrogen transporter genes increased significantly, but responses of the three genes putatively associated with symbiosis signaling/nodule initiation were mixed. There were infrequent responses of genes coding for an ABA-activated protein kinase or a gibberellin-regulated protein, but an ethylene-responsive element-binding factor showed increased expression in various treatments and sampling times. Three auxin-responsive genes and three cytokinin-responsive genes showed varied responses to ammonium and nitrate. This study indicates that low concentrations of ammonium stimulate nodulation in M. truncatula, but the data were inconclusive in verifying the hypothesis that a relatively high ratio of cytokinin to auxin in roots may be an underlying mechanism in this stimulation of nodulation.

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Section: Discussionmentioning

confidence: 99%

Stimulation of nodulation inMedicago truncatulaby low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Fei

Vessey

2009

Physiologia Plantarum

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“…Flavonoids function in various plant development processes such as pigmentation, pollen germination, auxin transport, UV protection, interactions with microbes in the roots of legumes [47][48][49][50], and in response to wounding and pathogen invasion [51]. Chalcone reductase (CHR) is a key enzyme in flavonoid and isoflavonone synthesis.…”

Section: Resultsmentioning

confidence: 99%

Comparative gene expression analysis in stems of <i>Dolichos purpureus</i> and <i>Arabidopsis thaliana</i>

Yan¹,

Wang²,

Chu³

et al. 2012

AJMB

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The outermost layer epidermis of a plant stem plays an important role in protection and environmentsensing. The mechanisms of sensing and response to the environment through the stem epidermis remain unclear. Here we report enriched expression of genes involved in stress resistance and signal transduction functions in the stem epidermis of both D. purpureus and A. thaliana by cDNA cloning and QPCR in D. purpureus and by analysis using dataset from a genome-wide comparison with cDNAs differentially expressed between the epidermis and inner parts of top and base stem in A. thaliana. Among 188 cDNAs from the stem epidermis of D. purpureus, 13% and 17% were related to signal transduction and defense respectively. Most of them were up-regulated more in the stem epidermis than the inner stem, as well as in A. thaliana. Also, the distribution of the numbers and specificities of up-regulated genes related to signal transduction and regulatory networks in the epidermis and inner stem revealed the possibility of positional differences in regulation. The results revealed the importance of the epidermis in signal transduction and plant defence.

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“…The activation of CHS gene as a response to wounding has been found earlier in Medicago sativa leaves (Sallaud et al 1995), Picea glauca needles (Richard et al 2000), and in Phaseolus vulgaris hypocotyls (Lawton and Lamb 1987) in addition to some other plant species Paiva 1995, De Bruxelles andRoberts 2001). On the other hand, Haukioja (2003) found no clear correlation between the accumulation of phenolic compounds and the consumption of mountain birch leaves by herbivores.…”

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confidence: 93%

Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves

Jaakola¹,

Koskimäki²,

Riihinen³

et al. 2008

Biol. plant.

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The influence of artificial wounding on biosynthesis of flavonoids and hydroxycinnamic acids was studied in bilberry leaves using two separate wounding experiments. In the first experiment bilberry leaves were wounded by cutting. The expression of the first gene from flavonoid pathway, chalcone synthase (CHS) and a wound induced pathogenesis related PR-10 gene was analysed from samples collected immediately and after 3, 6, 24 h and 4 d from the wounding treatment. In the second experiment annual shoots were removed. Proanthocyanidins, flavonol glycosides and hydroxycinnamic acids were quantified in leaf samples after 0 -5 d (experiment 1) and 5 weeks (experiment 2) from the treatment. In the first experiment, no change was observed in the expression of CHS whereas increase in expression of PR-10 gene was detected after 6 h of wounding treatment. In both experiments, the contents of flavonol glycosides and hydroxycinnamic acids were not influenced by the wounding treatment and the contents of proanthocyanidins were decreased.

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Differential expression of cDNA coding for chalcone reductase, a key enzyme of the 5-deoxyflavonoid pathway, under various stress conditions in Medicago sativa

Cited by 29 publications

References 29 publications

Stimulation of nodulation inMedicago truncatulaby low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Stimulation of nodulation inMedicago truncatulaby low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Comparative gene expression analysis in stems of <i>Dolichos purpureus</i> and <i>Arabidopsis thaliana</i>

Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves

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Differential expression of cDNA coding for chalcone reductase, a key enzyme of the 5-deoxyflavonoid pathway, under various stress conditions in Medicago sativa

Cited by 29 publications

References 29 publications

Stimulation of nodulation inMedicago truncatulaby low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Stimulation of nodulation inMedicago truncatulaby low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Comparative gene expression analysis in stems of &lt;i&gt;Dolichos purpureus&lt;/i&gt; and &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves

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Comparative gene expression analysis in stems of <i>Dolichos purpureus</i> and <i>Arabidopsis thaliana</i>