Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells

Singh, Nisha; Chakraborty, Raja; Bhullar, Rajinder P.; Chelikani, Prashen

doi:10.1016/j.bbrc.2014.02.140

Cited by 54 publications

(70 citation statements)

References 23 publications

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“…Recently, several studies have also described the expression and roles of TAS2Rs in several cancer cell lines. For example, Singh and colleagues discovered the expression of TAS2R4 differed between metastatic breast cancer and non-cancerous epithelial cell lines [6], and Gaida and colleagues demonstrated the expression and localization of TAS2R38 in a pancreatic cancer cell line [8]. However, little is known about the role of TAS2Rs in the cancer stemness, migration, and invasion.…”

Section: Discussionmentioning

confidence: 99%

“…Putative functions of TAS2Rs have also been related to various diseases, including severe asthma and cancer [5–7]. In breast and pancreatic cancers, targeting of TAS2Rs has shown potential to serve as a novel anti-cancer strategy [6, 8]. To date, there have been limited studies of bitter taste receptors, although it is generally accepted that sensing of bitter taste are associated with a self-defense system by which humans can protect themselves against the ingestion of potentially harmful, dangerous, and toxic substances [9].…”

Section: Introductionmentioning

confidence: 99%

“…Agonists of TAS2R14, including quercetin and naringenin, have exhibited anti-cancer activities in various cancer types, including NB cells [24, 26, 27]. Recently, it has been reported that differential expression of TAS2Rs were observed in non-cancerous breast epithelial versus breast cancer cells, and TAS2Rs were down-regulated in breast cancer cells [6]. However, the anti-cancer stemness and anti-metastatic effects of TAS2Rs remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells

et al. 2017

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Neuroblastoma (NB) originates from immature neuronal cells and currently has a poor clinical outcome. NB cells possess cancer stem cells (CSCs) characteristics that facilitate the initiation of a tumor, as well as its metastasis. Human bitter taste receptors, referred to as TAS2Rs, are one of five types of basic taste receptors and they belong to a family of G-protein coupled receptors. The recent finding that taste receptors are expressed in non-gustatory tissues suggest that they mediate additional functions distinct from taste perception. While it is generally admitted that the recognition of bitter tastes may be associated with a self-defense system to prevent the ingestion of poisonous food compounds, this recognition may also serve as a disease-related function in the human body. In particular, the anti-cancer stemness and invasion effects of TAS2Rs on NB cells remain poorly understood. In the present study, endogenous expression of TAS2R8 and TAS2R10 in SK-N-BE(2)C and SH-SY5Y cells was examined. In addition, higher levels of TAS2R8 and TAS2R10 expression were investigated in more differentiated SY5Y cells. Both TAS2Rs were up-regulated following the induction of neuronal cell differentiation by retinoic acid. In addition, ectopic transfection of the two TAS2Rs induced neurite elongation in the BE(2)C cells, and down-regulated CSCs markers (including DLK1, CD133, Notch1, and Sox2), and suppressed self-renewal characteristics. In particular, TAS2RS inhibited tumorigenicity. Furthermore, when TAS2Rs was over-expressed, cell migration, cell invasion, and matrix metalloproteinases activity were inhibited. Expression levels of hypoxia-inducible factor-1α, a well-known regulator of tumor metastasis, as well as its downstream targets, vascular endothelial growth factor and glucose transporter-1, were also suppressed by TAS2Rs. Taken together, these novel findings suggest that TAS2Rs targets CSCs by suppressing cancer stemness characteristics and NB cell invasion, thereby highlighting the chemotherapeutic potential of bitter taste receptors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells

et al. 2017

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“…However, recent studies on TAS2Rs confirmed that these receptors are expressed in many extra-oral tissues, including the respiratory system, gastrointestinal tract, brain, reproductive tissues and airways [57,66,69]. Lee et al [70] demonstrated that TAS2R38 is involved in the operation of defence mechanisms that protect the respiratory system from microbes such as Pseudomonas aeruginosa and other Gram-negative bacteria.…”

Section: Bitter Taste Receptors In Extra-oral Tissuesmentioning

confidence: 92%

“…Similarly to TAS1R, TAS2R is in a family of glycoprotein receptors [1] capable of homo-and heterooligomer formation [50]. The presence of TAS2R was demonstrated in the entire oral cavity and in the lungs, tissues of the alimentary tract, sexual organs and brain [65,66]. Receptor cells of bitter taste are equipped with many bitter taste receptors.…”

Section: Activation Of Receptor Cells For Umami Sweet and Bitter Tastesmentioning

confidence: 97%

How taste works: cells, receptors and gustatory perception

Kikut-Ligaj

Trzcielińska-Lorych

2015

Cellular and Molecular Biology Letters

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The sensitivity of taste in mammals varies due to quantitative and qualitative differences in the structure of the taste perception organs. Gustatory perception is made possible by the peripheral chemosensory organs, i.e., the taste buds, which are distributed in the epithelium of the taste papillae of the palate, tongue, epiglottis, throat and larynx. Each taste bud consists of a community of ~100 cells that process and integrate taste information with metabolic needs. Mammalian taste buds are contained in circumvallate, fungiform and foliate papillae and react to sweet, salty, sour, bitter and umami stimuli. The sensitivity of the taste buds for individual taste stimuli varies extensively and depends on the type of papillae and the part of the oral cavity in which they are located. There are at least three different cell types found in mammalian taste buds: type I cells, receptor (type II) cells and presynaptic (type III) cells. This review focuses on the biophysiological mechanisms of action of the various taste stimuli in humans. Currently, the best-characterized proteins are the Brought to you by |

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T2R bitter taste receptors regulate apoptosis and may be associated with survival in head and neck squamous cell carcinoma

et al. 2021

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Better management of head and neck squamous cell carcinomas (HNSCCs) requires a clearer understanding of tumor biology and disease risk. Bitter taste receptors (T2Rs) have been studied in several cancers, including thyroid, salivary, and GI, but their role in HNSCC has not been explored. We found that HNSCC patient samples and cell lines expressed functional T2Rs on both the cell and nuclear membranes. Bitter compounds, including bacterial metabolites, activated T2R‐mediated nuclear Ca2+ responses leading to mitochondrial depolarization, caspase activation, and ultimately apoptosis. Buffering nuclear Ca2+ elevation blocked caspase activation. Furthermore, increased expression of T2Rs in HNSCCs from The Cancer Genome Atlas is associated with improved overall survival. This work suggests that T2Rs are potential biomarkers to predict outcomes and guide treatment selection, may be leveraged as therapeutic targets to stimulate tumor apoptosis, and may mediate tumor‐microbiome crosstalk in HNSCC.

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Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells

Cited by 54 publications

References 23 publications

Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells

Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells

How taste works: cells, receptors and gustatory perception

T2R bitter taste receptors regulate apoptosis and may be associated with survival in head and neck squamous cell carcinoma

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