Differential expression of annexins I and II in normal and malignant human mammary epithelial cells

Schwartz‐Albiez, Reinhard; Koretz, K.; Möller, P.; Wirl, Gerhard

doi:10.1111/j.1432-0436.1993.tb00635.x

Cited by 44 publications

(33 citation statements)

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“…Therefore domes in the LA7 cultures correspond to the initial stage of mammary gland development, associated with the formation of tubules and of early alveoli during pregnancy. Consistent with these observations, as reported by Schwarz-Albiez and coworkers (23), the mammary alveolar epithelial cells are the most immunoreactive to annexin I. The LA7 line, therefore, when treated with dome inducers, appears to parallel a distinct stage of the mammary development in vivo.…”

Section: Discussionsupporting

confidence: 79%

“…In addition we show that the DMSO-induced domes express at a high level the annexin I and HSP90-␤ proteins, the expression of which also have been shown to be stage-specific during pregnancy and lactation in other in vitro models and in the animal in vivo (25,26). It has been shown that both of these genes are involved in the secretory and functional differentiation of the mammary gland in vivo (23,25,26); now we demonstrate that their expression in LA7 is required for the formation of domes. We therefore can conclude that the formation of domes corresponds to a specific stage in the lobulo-alveolar development of the mammary gland occurring during pregnancy and lactation.…”

Section: Discussionmentioning

confidence: 95%

“…They are annexin I and HSP90-␤, which are highly expressed in DMSO-induced LA7 cells. These two proteins appear also to be involved in the secretory differentiation process and in the developmental regulation of the mammary gland (23)(24)(25)(26). Interestingly, alveolar epithelial cells of the mammary gland display the highest level of annexin I expression, suggesting a specialized function of annexin I in these cells (23).…”

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confidence: 99%

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Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland

Zucchi

Bini

Albani

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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The study of the development of the mammary gland at the molecular level in the animal is difficult because of the complex tissue organization of the gland. We have previously developed an in vitro system for genetic analysis of mammary cell differentiation, based on the cell line LA7 clonally derived from a rat mammary adenocarcinoma. This cell line, after induction with DMSO, differentiates forming structures called domes. This process is under strict gene regulation, and we have previously identified several of the genes involved. In the present paper, we have defined the meaning of dome formation in relation to mammary development, by showing that treatment of LA7 cells with the lactogenic hormones hydrocortisone and prolactin induces dome formation; in the animal, these hormones precede and accompany milk production. Moreover, dome formation is accompanied by expression within the cells of the milk protein genes WDMN1 and ␤-casein, which are differentiation markers for the gland during pregnancy and lactation. We also show that two proteins, highly expressed in the mammary gland during lactation, HSP90-␤ and annexin I, are strongly expressed in DMSO-induced LA7 cells. Both proteins are essential in the formation of domes because when their synthesis is blocked by antisense RNA oligonucleotides, dome formation is abolished. Thus our in vitro system is a model for lobulo-alveolar development, and the genes identified in the pathway of dome formation are likely to be involved in the early differentiation steps occurring in the rat mammary gland during pregnancy and lactation.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland

Zucchi

Bini

Albani

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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“…In human BRCA1-associated breast carcinoma, Annexin A8 expression did not correlate with apoptosis; 8 In the human breast, Annexin A8 was expressed in the lobular and ductal luminal epithelium and some basal cells of major ducts and was rarely expressed in tumor cells. In this respect, Annexin A8 behaved similarly to Annexin A1, which is localized in acini and ductal basal cells of the normal human breast but not in tumor tissue (36). However, Annexin A1 was found to bind to CK8 and CK18, two luminal cell marker proteins (37), whereas Annexin A8 RNA expression was linked with the expression of CK5 and CK17 in recent microarray studies (15 -17).…”

Section: Discussionmentioning

confidence: 99%

Annexin A8 Is Up-Regulated During Mouse Mammary Gland Involution and Predicts Poor Survival in Breast Cancer

Stein

Price

Morris

et al. 2005

Clinical Cancer Research

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Purpose: Microarray studies have linked Annexin A8 RNA expression to a ''basal cell^like'' subset of breast cancers, including BRCA1-related cancers, that are characterized by cytokeratin 5 (CK5) and CK17 expression and show poor prognosis. We assessed Annexin A8's contribution to the overall prognosis and its expression in normal, benign, and cancerous tissue and addressed Annexin A8's physiologic role in the mammary gland. Experimental Design: Using microarrays and reverse transcription-PCR, the Annexin A8 expression was studied during mouse mammary gland development and in isolated mammary structures. Reverse transcription-PCR on cultured human luminal and basal cells, along with immunocytochemistry on normal and benign breast tissues, was used for cellular localization. Annexin A8's prognostic relevance and its coexpression with CK5 were assessed on tissue arrays of 1,631cases of invasive breast cancer. Coexpression was further evaluated on a small cohort of 14 BRCA1-related breast cancers. Results: Annexin A8 was up-regulated during mouse mammary gland involution and in pubertal ductal epithelium. Annexin A8 showed preferred expression in cultured basal cells but predominant luminal expression in normal human breast tissue in vivo. Hyperplasias and in situ carcinomas showed a strong staining of basal cells. Annexin A8 expression was significantly associated with grade (P < 0.0001), CK5 (P < 0.0001), and estrogen receptor status (P < 0.0001); 85.7% BRCA1-related breast tumors coexpressed Annexin A8 and CK5. Conclusion: Annexin A8 is involved in mouse mammary gland involution. In humans, it is a luminally expressed protein with basal expression in cell culture and in hyperplasia/ductal carcinoma in situ. Expression in invasive breast carcinomas has a significant effect on survival (P = 0.03) but is not independent of grade or CK5.

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“…In addition, all three ERM proteins are ubiquitously expressed in most cultured cells (21,22,52). However, some tissue-specific requirements for individual ERM proteins probably do exist as one or more of the ERM proteins can be absent or predominate in certain tissues (53)(54)(55).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Interaction between ELMO1 and ERM Proteins

Grimsley

Haney

et al. 2006

Journal of Biological Chemistry

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ERMs are closely related proteins involved in cell migration, cell adhesion, maintenance of cell shape, and formation of microvilli through their ability to cross-link the plasma membrane with the actin cytoskeleton. ELMO proteins are also known to regulate actin cytoskeleton reorganization through activation of the small GTPbinding protein Rac via the ELMO-Dock180 complex. Here we showed that ERM proteins associate directly with ELMO1 as purified recombinant proteins in vitro and at endogenous levels in intact cells. We mapped ERM binding on ELMO1 to the N-terminal 280 amino acids, which overlaps with the region required for binding to the GTPase RhoG, but is distinct from the C-terminal Dock180 binding region. Consistent with this, ELMO1 could simultaneously bind both radixin and Dock180, although radixin did not alter Rac activation via the Dock180-ELMO complex. Most interestingly, radixin binding did not affect ELMO binding to active RhoG and a trimeric complex of active RhoG-ELMO-radixin could be detected. Moreover, the three proteins colocalized at the plasma membrane. Finally, in contrast to most other ERM-binding proteins, ELMO1 binding occurred independently of the state of radixin C-terminal phosphorylation, suggesting an ELMO1 interaction with both the active and inactive forms of ERM proteins and implying a possible role of ELMO in localizing or retaining ERM proteins in certain cellular sites. Together these data suggest that ELMO1-mediated cytoskeletal changes may be coordinated with ERM protein crosslinking activity during dynamic cellular functions.

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Differential expression of annexins I and II in normal and malignant human mammary epithelial cells

Cited by 44 publications

References 44 publications

Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland

Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland

Annexin A8 Is Up-Regulated During Mouse Mammary Gland Involution and Predicts Poor Survival in Breast Cancer

Characterization of a Novel Interaction between ELMO1 and ERM Proteins

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