Differential Expression of &amp;lt;italic&amp;gt;PPARγ&amp;lt;/italic&amp;gt;, &amp;lt;italic&amp;gt;FASN&amp;lt;/italic&amp;gt;, and &amp;lt;italic&amp;gt;ACADM&amp;lt;/italic&amp;gt; Genes in Various Adipose Tissues and &amp;lt;italic&amp;gt;Longissimus dorsi&amp;lt;/italic&amp;gt; Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle

Ji, Shuang; Yang, Runjun; Lu, Chao; Qiu, Zhengyan; Yan, Chunri; Zhao, Zhihui

doi:10.5713/ajas.2013.13422

Cited by 23 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differential expressions of FASN in muscle were reported within the same breed divergent for IMF content (in pig; Lim et al., ) and meat tenderness (in cattle; Zhao, Tian, et al., ; in pig; Zhao, Li, et al., ). Breed‐specific expression of FASN was also observed in pigs (Duran‐Montge, Theil, Lauridsen, & Esteve‐Garcia, ), cattle (Ji et al., ), and chicken (Cui et al., ). Consistent with previous studies performed by Ponsuksili, Murani, Walz, Schwerin, and Wimmers () and Miao et al.…”

Section: Discussionmentioning

confidence: 92%

“…Differential expressions of FASN in muscle were reported within the same breed divergent for IMF content (in pig; Lim et al, 2017) and meat tenderness (in cattle; Zhao, Tian, et al, 2012;in pig;Zhao, Li, et al, 2012). Breed-specific expression of FASN was also observed in pigs (Duran-Montge, Theil, Lauridsen, & Esteve-Garcia, 2009), cattle (Ji et al, 2014), and chicken (Cui et al, 2012). Consistent with previous studies performed by Ponsuksili, Murani, Walz, Schwerin, and Wimmers (2007) and Miao et al (2010) on pigs differing in fatness traits, which revealed that the expression of FASN was up-regulated in the fatter pig breed than in the leaner breed, our present study identified significantly increased expression of FASN in both the liver and the breast muscle in male SQ squabs characterized with significantly higher IMF content.…”

Section: Variability Of Expression Levels Of Genes In Relation To Imfmentioning

confidence: 92%

See 1 more Smart Citation

Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

Chen³

et al. 2018

Animal Physiology Nutrition

View full text Add to dashboard Cite

In this study, meat quality traits were compared between squabs from two pigeon breeds: one Chinese indigenous breed, the Shiqi (SQ) meat-type pigeon, and an imported breed, the white king (WK) meat-type pigeon. Breed differences were detected in the content of intramuscular fat (IMF) in the breast muscle. SQ squabs had significantly higher IMF content than the WK birds. The shear force value (an objective measure of meat tenderness) of SQ birds was also relatively lower than that of the WK squabs. Further analysis of fatty acids profile revealed that SQ squabs exhibited significant advantage in the synthesis of polyunsaturated fatty acids, while WK squabs were significantly higher in the sum of monounsaturated fatty acids. Breast muscle in the SQ squabs was also significantly higher in the ratio of polyunsaturated fatty acids to saturated fatty acids, as well as the sum of omega 6 fatty acids. Variability of expression levels of functional genes in relation to fat accumulation and meat tenderness was analysed by qRT-PCR. Gene expression analyses showed that the hepatic expression of LPL (lipoprotein lipase), FABP4 (fatty acid-binding protein 4), and CAPN2 (calpain-2) were significantly higher in the SQ squabs. In the breast muscle tissue, the FABP3 (fatty acid-binding protein 3) and CAPN2mRNA abundance was significantly higher in SQ squabs. Our results suggested that these differentially expressed genes might be candidate genes used in the programmes of targeted selection for squabs with higher IMF content, tender meat, and more favourable fatty acids composition.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Variability Of Expression Levels Of Genes In Relation To Imfmentioning

confidence: 92%

Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

Chen³

et al. 2018

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

“…This would be consistent with the results obtained here, where, aside from the mammary gland, higher levels of expression of FASN were only seen in the subcutaneous fat. High levels of FASN gene expression have previously been reported in lactating and non-lactating buffalo ( B. bubalis ) mammary gland and subcutaneous fat tissue [28], although in humans, FASN was reported to be expressed in all tissues studied, with the highest levels observed in the liver and lungs [29]. …”

Section: Discussionmentioning

confidence: 99%

Variation in the Fatty Acid Synthase Gene (FASN) and Its Association with Milk Traits in Gannan Yaks

Shi

Jiang

Chen

et al. 2019

Animals

View full text Add to dashboard Cite

Simple SummaryThe yak (Bos grunniens) is a symbolic animal living in alpine climates (between 2000 to 5000 m) in the Qinghai–Tibetan Plateau. More than 13 million domestic yaks provide the basic resources (such as meat, milk, hair, transportation, and dung for fuel) necessary for Tibetans and nomads in China. While yak milk production is not elevated, yak milk is superior to dairy cow milk in nutrient composition (protein and fat). Fatty acid synthase (FASN) is an enzyme involved in the synthesis of fatty acids (FA) and plays a central role in de novo lipogenesis in mammals. However, there have been few reports on the effects of the FASN gene on the milk traits of yak. Our study elucidated the tissue expression of the yak FASN gene and the association of variants and haplotypes in the gene with milk fat percentage and total milk solid percentage. The results provide guidance for the molecular-assisted selection of milk traits in yaks.AbstractFatty acid synthase (FASN) is an enzyme involved in the synthesis of fatty acids (FA) and plays a central role in de novo lipogenesis in mammals. This study was conducted to ascertain the relative level of expression of the FASN gene (FASN) in tissues from the yak (Bos grunniens), and to search for variation in two regions of yak FASN using polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) analyses; it also ascertains whether that variation is associated with yak milk traits. The gene was found to be expressed in twelve tissues, with the highest expression detected in the mammary gland, followed by subcutaneous fat tissue. Two regions of the gene were analyzed in 290 Gannan yaks: A region spanning exon 24-intron 24 and a region spanning exon 34. These regions both produced two PCR-SSCP patterns, which, upon sequencing, represented different DNA sequences. This sequence variation resulted from the presence of three nucleotide substitutions: c.4296+38C/T (intron 24), c.5884A/G, and c.5903G/A, both located in exon 34. The exon 34 substitutions would result in the amino acid substitutions p.Thr1962Ala and p.Gly1968Glu if expressed. Four haplotypes spanning from the exon 24-intron 24 region to exon 34 were identified. Of these, two were common (A1-A2 and B1-A2), and two were rare (A1-B2 and B1-B2) in the yaks investigated. The presence of A1-A2 was associated with an increase in milk fat content (p = 0.050) and total milk solid content (p = 0.037), while diplotype A1-A2/B1-A2 had a higher milk fat content (p = 0.038) than the other diplotypes. This study suggests that further characterization of the FASN gene might provide for an improved understanding of milk traits in yaks.

show abstract

“…In cumulus cells of the PAPP‐A treatment group, AGPAT9 , FASN , EGFR , HAS2 , and IMPDH1 were downregulated, whereas VNN1 was upregulated. Regarding genes related to lipid metabolism, AGPAT9 participates in triacylglycerol synthesis and membrane‐lipid biosynthesis (Körbes, Kulcheski, Margis, Margis‐Pinheiro, & Turchetto‐Zolet, ; Zeng et al, ), whereas the FASN gene is involved in long fatty acid biosynthesis (Ji et al, ; Yang, Cappello, & Wang, ). IGF‐1 plays an important role in the development and differentiation of white and brown adipose tissue (Blüher, Kratzsch, & Kiess, ; Sudano et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…participates in triacylglycerol synthesis and membrane-lipid biosynthesis (Körbes, Kulcheski, Margis, Margis-Pinheiro, & Turchetto-Zolet, 2016;Zeng et al, 2017), whereas the FASN gene is involved in long fatty acid biosynthesis (Ji et al, 2014;Yang, Cappello, & Wang, 2015).…”

Section: Groupsmentioning

confidence: 99%

Use of pregnancy‐associated plasma protein‐A during oocyte in vitro maturation increases IGF‐1 and affects the transcriptional profile of cumulus cells and embryos from Nelore cows

Giroto

Fontes

Franchi

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

Insulin‐like growth factor 1 (IGF‐1) activity is established by the regulation of IGF binding protein activity, which blocks IGF‐1 functions, whereas pregnancy‐associated plasma protein‐A (PAPP‐A) improves IGF‐1 bioavailability and facilitates binding to IGF receptors. To further extend our understanding of the effect of exogenous PAPP‐A on bovine embryo production, we added this protein during in vitro maturation of cumulus‐oocyte complexes (COCs); moreover, we assessed its effects on IGF‐1 quantity in the maturation medium, embryonic yield and postwarming survival, blastocyst quality, and transcript abundance. Bovine COCs were matured in a serum‐free medium, either with PAPP‐A supplementation (100 ng/ml) or without (control). The treatment group produced higher IGF‐1 concentrations in the maturation medium; however, showed no difference on cleavage, blastocysts rates, and embryonic survival 3 and 24 hr postcryopreservation. Regarding gene expression, VNN1 was upregulated, whereas AGPAT9, FASN, EGFR, HAS2, and IMPDH1 were downregulated in PAPP‐A treated. PAPP‐A treated, CPT2, DNMT3A, and TFAM were upregulated, whereas ATF4 and IFITM3 were downregulated. We concluded that although the addition of PAPP‐A did not affect embryo yield and blastocyst survival, higher IGF‐1 levels may affect embryo competence through differential expression of genes involved in lipid metabolism, oocyte competence, and mitochondrial function.

show abstract

Differential Expression of <italic>PPARγ</italic>, <italic>FASN</italic>, and <italic>ACADM</italic> Genes in Various Adipose Tissues and <italic>Longissimus dorsi</italic> Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle

Cited by 23 publications

References 32 publications

Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

Variation in the Fatty Acid Synthase Gene (FASN) and Its Association with Milk Traits in Gannan Yaks

Use of pregnancy‐associated plasma protein‐A during oocyte in vitro maturation increases IGF‐1 and affects the transcriptional profile of cumulus cells and embryos from Nelore cows

Contact Info

Product

Resources

About