Differential expression of ABH histo-blood group antigens and LAMPs in infantile hemangioma

Sarafian, Victoria; Dikov, Dorian; Karaivanov, M; Belovejdov, V.; Stefanova, Penka

doi:10.1007/s10735-006-9017-x

Cited by 6 publications

(12 citation statements)

References 29 publications

(27 reference statements)

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“…Furthermore, lysosome-associated membrane proteins (LAMPs), proteins associated with autolysosome formation, were found in immature endothelium of proliferating infantile hemangioma, a tumor of the microvasculature characterized by aggressive angiogenesis during infancy. LAMPs were increased in involuting hemangioma, which might be associated with augmented autophagy required for tissue remodeling during tumor involution (36). Additionally, cathepsin D, a lysosomal hydrolase associated with autophagy, has been found to enhance angiogenesis of cancer cells probably by degrading extracellular matrix (20).…”

Section: Discussionmentioning

confidence: 99%

Role of autophagy in angiogenesis in aortic endothelial cells

Teng

Guan³

et al. 2012

American Journal of Physiology-Cell Physiology

154

126

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Angiogenesis plays critical roles in the recovery phase of ischemic heart disease and peripheral vascular disease. An increase in autophagy is protective under hypoxic and chronic ischemic conditions. In the present study we determined the role of autophagy in angiogenesis. 3-Methyladenine (3-MA) and small interfering RNA (siRNA) against ATG5 were used to inhibit autophagy induced by nutrient deprivation of cultured bovine aortic endothelial cells (BAECs). Assays of BAECs tube formation and cell migration revealed that inhibition of autophagy by 3-MA or siRNA against ATG5 reduced angiogenesis. In contrast, induction of autophagy by overexpression of ATG5 increased BAECs tube formation and migration. Additionally, inhibiting autophagy impaired vascular endothelial growth factor (VEGF)-induced angiogenesis. However, inhibition of autophagy did not alter the expression of pro-angiogenesis factors such as VEGF, platelet-derived growth factor, or integrin αV. Furthermore, autophagy increased reactive oxygen species (ROS) formation and activated AKT phosphorylation. Inhibition of autophagy significantly decreased the production of ROS and activation of AKT but not of extracellular regulated kinase, whereas overexpression of ATG5 increased cellular ROS production and AKT activation in BAECs. Inhibition of AKT activation or ROS production significantly decreased the tube formation induced by ATG5 overexpression. Here we report a novel observation that autophagy plays an important role in angiogenesis in BAECs. Induction of autophagy promotes angiogenesis while inhibition of autophagy suppresses angiogenesis, including VEGF-induced angiogenesis. ROS production and AKT activation might be important mechanisms for mediating angiogenesis induced by autophagy. Our findings indicate that targeting autophagy may provide an important new tool for treating cardiovascular disease.

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Section: Discussionmentioning

confidence: 99%

Role of autophagy in angiogenesis in aortic endothelial cells

Teng

Guan³

et al. 2012

American Journal of Physiology-Cell Physiology

154

126

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“…It was shown that a histo-blood group B-like antigen is strongly upregulated during rat mammary gland involution, implying that this antigen might either provide a survival function and/ or be expressed in epithelial cells destined to remodel mammary ducts (Mengwasser and Sleeman 2001). These molecules are also implicated in the death of endothelial cells in regressing infantile hemangioma (Sarafian et al 2005). The current study indicates that HGBAs are expressed not only in human and animal tissues undergoing cell death but also in cell cultures exposed to toxic agents.…”

Section: Discussionmentioning

confidence: 99%

“…The indirect immunoperoxidase technique using the universal LSAB-2 kit (Dako, Glostrup, Denmark; cat no. K0672) with chromogen aminoethylcarbazole (AEC) was carried out according to previously described protocols (Sarafian et al 2005). Cell proliferation was assessed using monoclonal antibodies with defined specificity to human antigens: PCNA (proliferative cell nuclear antigen) (Dako; cat no.…”

Section: Immunocytochemistrymentioning

confidence: 99%

“…Their precise immunobiological role in cell differentiation, proliferation, and death is still unclear. We have recently demonstrated that HBGAs are related to proliferation and death of endothelial cells (Sarafian et al 2005). It is assumed that they enhance the resistance to apoptosis and facilitate escape from immune control of rat colon carcinoma cells (Marionneau et al 2002).…”

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confidence: 99%

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Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers

et al. 2007

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The present work is focused on examining the effect of the structurally similar dental monomers bis-GA and bis-GMA on the expression of histo-blood group antigens (HBGAs) in comparison with fibroblast vitality and proliferation. The fibroblast cell line McCoy-Plovdiv was cultivated in a serum-free medium and was treated with both monomers. Cell vitality was measured by the crystal violet test. Mitotic index and cell morphology were assessed. An immunocytochemical technique was applied to follow the expression of proliferative antigens PCNA and Ki-67 and of HBGA. The expression level of HBGA was measured by an improved pixel selection algorithm with proprietary software. The lowest concentration of 2.5 micromol/L did not significantly affect morphology, vitality, or proliferation activity. Interestingly, the quantitative analysis revealed augmented expression of HBGA B at 2.5 micromol/L. The higher concentrations of the dental monomers reduced cell vitality and mitotic indices and altered proliferative antigen expression. Bis-GA proved to be more toxic than bis-GMA and caused more prominent alterations including greater enhancement of HBGA B expression. We present novel evidence for altered expression of proliferative antigens and enhanced expression of HBGA B in fibroblasts treated with dental monomers bis-GA and bis-GMA suggesting that these substances affect cell morphology, proliferative activity, and antigenic profile.

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“…As endothelial cells are continuously hit by circulating cells, they are believed to be among the cells most frequently wounded and repaired (1). Our previous investigations suggest that ABH histo‐blood group antigens (HBGAs) might be used as markers for endothelial differentiation of embryonic mesenchymal cells (2), as well as indicators of endothelial cells in vascular tumors such as infantile hemangioma (3). As there are few investigations of these molecules in the granulation tissue it seemed interesting to follow their expression in sprouting capillaries.…”

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confidence: 99%

LAMPs and ABH histo‐blood group antigens in granulation tissue

Sarafian

Dikov²

2007

APMIS

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Endothelial cells are major participants in angiogenic processes accompanying wound repair. The functions of ABH histo-blood group antigens (HBGAs) and lysosome-associated membrane proteins LAMP-1 and LAMP-2 in endothelial cells of granulation tissue are currently unkown. Here we hypothesize that HBGAs and LAMPs enrich the phenotypic characteristics of endothelial cells and might be implicated in the plasticity of granulation tissue. Immunohistochemistry revealed permanent expression of HBGAs in the cytoplasm of endothelial cells of all sprouting capillaries regardless of the organ examined. A modulation in both the localization and the intensity of the signal for LAMPs was observed. Interestingly, LAMP-1 showed a more intensive staining compared to LAMP-2. LAMP-1 was found in the cytoplasm, as well as on plasma membranes of endothelial cells. We present the first comparative immunohistochemical study of the expression of HBGA and LAMPs in endothelial cells of granulation tissue. Novel evidence for modulating LAMP reactivity is reported. Our results suggest that both glycoconjugates might contribute to the process of neoangiogenesis and tissue remodeling in wound healing.

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Differential expression of ABH histo-blood group antigens and LAMPs in infantile hemangioma

Cited by 6 publications

References 29 publications

Role of autophagy in angiogenesis in aortic endothelial cells

Role of autophagy in angiogenesis in aortic endothelial cells

Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers

LAMPs and ABH histo‐blood group antigens in granulation tissue

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