Differential expression of a subset of ribosomal protein genes in cell lines derived from human nasopharyngeal epithelium

Sim, Edmund Ui Hang; Ang, Chow Hiang; Ng, Ching Ching; Lee, Choon Weng; Narayanan, K.

doi:10.1038/jhg.2009.124

Cited by 19 publications

(19 citation statements)

References 10 publications

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“…While this expression phenomenon reinforced the fact that they are likely candidates of NPC-associated RP factors, their expression behaviors contradict our previous report [7] of downregulation in NPC cells. Such discrepancy is difficult to explain, but, needless to say, our current findings are more definitive due to the greater accuracy of quantitative RT-PCR over semiquantitative strategy.…”

Section: Discussioncontrasting

confidence: 51%

“…Early evidence of RP genes involvement in NPC was limited to RPeS26 (RPS26), RPeS27 (RPS27), RPuS19 (RPS15), RPeL27 (RPL27), RPeL43 (RPL37a), and RPeL41 (RPL41) [5–7]. Albeit providing information on NPC-associated RP genes, these preliminary findings are largely speculative due to analysis that are semiquantitative in nature and/or confined to assessment at transcript level.…”

Section: Introductionmentioning

confidence: 99%

“…Its upregulation in NPC samples was also not evaluated at protein level. The three RP genes, RPeL27, RPeL43, and RPeL41, were identified to be associated with NPC from a study that employed semiquantitative RT-PCR assay of all RP genes encoding products for the large ribosome subunit [7]. Their underexpression in NPC cell lines compared to normal epithelial cell line remains to be reconfirmed using quantitative RT-PCR and/or Western Blot analysis.…”

Section: Introductionmentioning

confidence: 99%

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Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Sim

Chan

et al. 2016

Disease Markers

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Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes' involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (RPeL27, RPeL41, and RPeL43) between cell lines derived from tumor and normal nasopharyngeal epithelium. However, the results therein were based on a semiquantitative assay, thus preliminary in nature. Herein, we provide findings of a deeper analysis of these three genes in the context to nasopharyngeal carcinoma (NPC) tumorigenesis. Their expression patterns were analyzed in a more quantitative manner at transcript level. Their protein expression levels were also investigated. We showed results that are contrary to previous report. Rather than downregulation, these genes were significantly overexpressed in NPC cell lines compared to normal control at both transcript and protein levels. Nevertheless, their association with NPC has been established. Immunoprecipitation pulldown assays indicate the plausible interaction of either RPeL27 or RPeL43 with POTEE/TUBA1A and ACTB/ACTBL2 complexes. In addition, RPeL43 is shown to bind with MRAS and EIF2S1 proteins in a NPC cell line (HK1). Our findings support RPeL27, RPeL41, and RPeL43 as potential markers of NPC and provide insights into the interaction targets of RPeL27 and RPeL43 proteins.

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Section: Discussioncontrasting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Sim

Chan

et al. 2016

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“…Rp have been found overexpressed in cancer, for example rpL19, rpL7a, rpL37 in prostate cancer24 rpL15 and rpL19 in gastric cancer2526. The expression of other rp was found reduced as rpL27, rpL37, rpL41 in a subset of cell lines derived from nasopharyngeal carcinomas27. Changes in the expression levels of rps have been used as prognostic or predictive values to distinguish between normal and cancer cells.…”

Section: Discussionmentioning

confidence: 99%

rpL3 promotes the apoptosis of p53 mutated lung cancer cells by down-regulating CBS and NFκB upon 5-FU treatment

Russo

Saide

Cagliani

et al. 2016

Sci Rep

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5-FU is a chemotherapy drug commonly used for the treatment of human cancers; however drug resistance represents a major challenge for its clinical application. In the present study, we reporte that rpL3 induced by 5-FU treatment in Calu-6 cells represses CBS transcription and reduces CBS protein stability leading to a decrease of CBS protein levels. rpL3 also regulates negatively the activation of NFκB by preventing NFκB nuclear translocation through IκB-α up-regulation. Furthermore, we demonstrate that rpL3 significantly enhances the apoptosis of 5-FU treated Calu-6 cells promoting the overexpression of the pro-apoptotic proteins Bax and the inhibition of the anti-apoptotic protein Bcl-2. We finally demonstrate that rpL3 potentiates 5-FU efficacy inhibiting cell migration and invasion. Our results suggest that combination of rpL3 and 5-FU is a promising strategy for chemotherapy of lung cancers lacking functional p53 that are resistant to 5-FU.

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“…Recent studies showed that the ribosomal protein large subunit (RPL) genes of RPL27, RPL37a and RPL41 are significantly downregulated in cell lines derived from nasopharyngeal carcinoma (NPC) compared to a derivative from normal nasopharyngeal epithelium [6], [7]. However, limited studies on protein-protein interaction activities have made the understanding of their extraribosomal roles difficult.…”

mentioning

confidence: 99%

Bioinformatics Analysis of the Ribosomal Proteins,RPL27, RPL37a and RPL41: 3-D Protein Modeling and Protein-protein Interaction Prediction

Chan¹,

Sim²

2013

IJBBB

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Abstract-Ribosomal proteins (RPs) are constituents of ribosome important for protein biosynthesis but likely to have extraribosomal functions. Many RPs are associated with various diseases and cancers. A previous study reported RPL27, RPL37a and RPL41 gene to be downregulated in nasopharyngeal carcinoma (NPC) derived cell lines compared to their normal counterpart. However, their actual physiological roles in organogenesis or tumorigenesis have not been properly defined. In this paper, we report on the findings of structural prediction of these three genes and infer their interactions with other proteins using structural neighbor prediction and molecular docking strategies. Our results revealed that RPL27 interact with SYNJ2 and UBC9. RPL27 is predicted to mediate RNA binding protein and deregulate sumolyation. RPL37a is suggested to interact with CTNNB1, SCMH1 and ATBF1. It is predicted to deregulate Wnt degradation pathway, inhibit β-catenin migration and regulate homeotic transcription. Our studies on RPL41 did not allow logical inference on possible interacting factors. Nevertheless, results on RPL27 and RPL37a provide rational data for the elucidation of their molecular activities.Index Terms-Protein modeling, extraribosomal functions, protein-protein interaction prediction, ribosomal protein.

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Differential expression of a subset of ribosomal protein genes in cell lines derived from human nasopharyngeal epithelium

Cited by 19 publications

References 10 publications

Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

rpL3 promotes the apoptosis of p53 mutated lung cancer cells by down-regulating CBS and NFκB upon 5-FU treatment

Bioinformatics Analysis of the Ribosomal Proteins,RPL27, RPL37a and RPL41: 3-D Protein Modeling and Protein-protein Interaction Prediction

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