Differential expression of 3Î²-HSD and mlncRNAs in response to abiotic stresses in Digitalis nervosa

Salimi, Vahideh; Maroufi, Asad; Majdi, Mohammad

doi:10.14715/cmb/2018.64.14.15

Cited by 2 publications

(3 citation statements)

References 44 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It indicated the adjacent relationship between 3β-HSD and three mlncRNAs through an unknown mechanism. Also, it revealed that the possible accumulation of mentioned transcripts in the aerial parts of plants was associated with secondary metabolites biosynthesis site [88]. Due to the fact that the expression levels of three mlncRNAs and 3β-HSD under stress conditions are similar and decreased, mlncRNAs are more possibly stress-responsive [88].…”

Section: Plos Onementioning

confidence: 99%

“…Identification of mlncRNAs. The mlncRNAs are considered as a subset of long noncoding RNAs (lncRNAs) and a new group of regulatory elements [88], which can be spliced, capped and polyadenylated [43]. In plants, mlncRNAs have regulatory roles in phosphate-starvation response, gender specific expression, nodulation, development, and response to hormones; also, they can affect cellular activity through specific sequences and RNA-folding structures [89].…”

Section: Plos Onementioning

confidence: 99%

“…SPS was involved in the biosynthesis of ubiquinone and plastoquinone [43]. In Digitalis nervosa, Salimi et al (2018) showed that the expression levels of Δ5-3β-hydroxysteroid dehydrogenase (a key gene in cardenolides biosynthesis, 3β-HSD), mlncRNA23, mlncRNA28, and mlncRNA30 across different tissues under normal conditions within leaves and roots were respectively high and low [88]. It indicated the adjacent relationship between 3β-HSD and three mlncRNAs through an unknown mechanism.…”

Section: Plos Onementioning

confidence: 99%

See 2 more Smart Citations

Identification of key genes involved in secondary metabolite biosynthesis in Digitalis purpurea

et al. 2023

View full text Add to dashboard Cite

The medicinal plant Digitalis purpurea produces cardiac glycosides that are useful in the pharmaceutical industry. These bioactive compounds are in high demand due to ethnobotany’s application to therapeutic procedures. Recent studies have investigated the role of integrative analysis of multi-omics data in understanding cellular metabolic status through systems metabolic engineering approach, as well as its application to genetically engineering metabolic pathways. In spite of numerous omics experiments, most molecular mechanisms involved in metabolic pathways biosynthesis in D. purpurea remain unclear. Using R Package Weighted Gene Co-expression Network Analysis, co-expression analysis was performed on the transcriptome and metabolome data. As a result of our study, we identified transcription factors, transcriptional regulators, protein kinases, transporters, non-coding RNAs, and hub genes that are involved in the production of secondary metabolites. Since jasmonates are involved in the biosynthesis of cardiac glycosides, the candidate genes for Scarecrow-Like Protein 14 (SCL14), Delta24-sterol reductase (DWF1), HYDRA1 (HYD1), and Jasmonate-ZIM domain3 (JAZ3) were validated under methyl jasmonate treatment (MeJA, 100 μM). Despite early induction of JAZ3, which affected downstream genes, it was dramatically suppressed after 48 hours. SCL14, which targets DWF1, and HYD1, which induces cholesterol and cardiac glycoside biosynthesis, were both promoted. The correlation between key genes and main metabolites and validation of expression patterns provide a unique insight into the biosynthesis mechanisms of cardiac glycosides in D. purpurea.

show abstract

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

See 1 more Smart Citation

Identification of key genes involved in secondary metabolite biosynthesis in Digitalis purpurea

et al. 2023

View full text Add to dashboard Cite

show abstract

Bioinformatics-assisted, integrated omics studies on medicinal plants

Meng

Wang

et al. 2019

Briefings in Bioinformatics

View full text Add to dashboard Cite

The immense therapeutic and economic values of medicinal plants have attracted increasing attention from the worldwide researchers. It has been recognized that production of the authentic and high-quality herbal drugs became the prerequisite for maintaining the healthy development of the traditional medicine industry. To this end, intensive research efforts have been devoted to the basic studies, in order to pave a way for standardized authentication of the plant materials, and bioengineering of the metabolic pathways in the medicinal plants. In this paper, the recent advances of omics studies on the medicinal plants were summarized from several aspects, including phenomics and taxonomics, genomics, transcriptomics, proteomics and metabolomics. We proposed a multi-omics data-based workflow for medicinal plant research. It was emphasized that integration of the omics data was important for plant authentication and mechanistic studies on plant metabolism. Additionally, the computational tools for proper storage, efficient processing and high-throughput analyses of the omics data have been introduced into the workflow. According to the workflow, authentication of the medicinal plant materials should not only be performed at the phenomics level but also be implemented by genomic and metabolomic marker-based examination. On the other hand, functional genomics studies, transcriptional regulatory networks and protein–protein interactions will contribute greatly for deciphering the secondary metabolic pathways. Finally, we hope that our work could inspire further efforts on the bioinformatics-assisted, integrated omics studies on the medicinal plants.

show abstract

Differential expression of 3Î²-HSD and mlncRNAs in response to abiotic stresses in Digitalis nervosa

Cited by 2 publications

References 44 publications

Identification of key genes involved in secondary metabolite biosynthesis in Digitalis purpurea

Identification of key genes involved in secondary metabolite biosynthesis in Digitalis purpurea

Bioinformatics-assisted, integrated omics studies on medicinal plants

Contact Info

Product

Resources

About