Differential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, Pectobacterium chrysanthemi

Jang, In-Chang; Park, Soo‐Young; Kim, Kee-Yeun; Kwon, Suk‐Yoon; Kim, Jong-Guk; Kwak, Sang‐Soo

doi:10.1016/j.plaphy.2004.04.002

Cited by 39 publications

(30 citation statements)

References 28 publications

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“…Additionally, three POX cDNAs were also isolated from dehydrated fibrous roots of sweetpotato (Kim et al 2008). Expression profiles of 14 antioxidant genes were performed as the first step toward understanding the physiological functions of each gene in response to various environmental stresses such as wounding, chilling, dehydration, stress-related chemicals and pathogen infection (Huh et al 1997;Kim et al 1999Kim et al , 2000Kim et al , 2007Kim et al , 2008Park et al 2003Park et al , 2004Jang et al 2004). However, little is known about expression patterns of POXs and APX genes during leaf development of sweetpotato.…”

Section: Introductionmentioning

confidence: 99%

Changes in activities of antioxidant enzymes and their gene expression during leaf development of sweetpotato

Kim

Lee

et al. 2009

Plant Growth Regul

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To understand the functions of antioxidant enzymes during leaf development in sweetpotato, we investigated the activities of several antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POX), ascorbate peroxidase (APX) and catalase (CAT). Significant increases were observed in the activities of SOD, POX and APX during the late stage of leaf development, whereas CAT activity increased during the early developmental stage. By RT-PCR analysis, various POX and APX genes showed differential expression patterns during leaf development. Four POX genes swpa3, swpa4, swpa6, swpb4 and one APX gene swAPX1 exhibited high levels of gene expression during the senescence stage of leaf development, but two POX genes, swpa1 and swpa7 were preferentially expressed at both the mature green and the late senescence stages of leaf development. These results indicate that hydrogen peroxide (H 2 O 2 )-related antioxidant enzymes are differentially regulated in the process of leaf development of sweetpotato.

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Section: Introductionmentioning

confidence: 99%

Changes in activities of antioxidant enzymes and their gene expression during leaf development of sweetpotato

Kim

Lee

et al. 2009

Plant Growth Regul

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“…Ten POD cDNA clones were isolated from sweetpotato cell cultures, and their expression proWles were determined as the initial step toward understanding the physiological functions of each POD gene in response to a variety of environmental stresses (Huh et al 1997;Kim et al 1999Kim et al , 2000Park et al 2003;Jang et al 2004). In the ten POD cDNAs, the transcript levels of swpa4 were shown to have been markedly increased under a variety of stress conditions, including methyl viologen (MV), H 2 O 2 , salt, chilling, wounding, and pathogenic bacteria Jang et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of sweetpotato swpa4 peroxidase results in increased hydrogen peroxide production and enhances stress tolerance in tobacco

Kim

Song

et al. 2007

Planta

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Plant peroxidases (POD) reduce hydrogen peroxide (H 2 O 2 ) in the presence of an electron donor. Extracellular POD can also induce H 2 O 2 production and may perform a signiWcant function in responses to environmental stresses via the regulation of H 2 O 2 in plants. We previously described the isolation of 10 POD cDNA clones from cell cultures of sweetpotato (Ipomoea batatas). Among them, the expression of the swpa4 gene was profoundly induced by a variety of abiotic stresses and pathogenic infections (Park et al. in Mol Gen Genome 269:542-552 2003; Jang et al. in Plant Physiol Biochem 42:451-455 2004). In the present study, transgenic tobacco (Nicotiana tabacum) plants overexpressing the swpa4 gene under the control of the CaMV 35S promoter were generated in order to assess the function of swpa4 in planta. The transgenic plants exhibited an approximately 50-fold higher POD speciWc activity than was observed in control plants. Both transient expression analysis with the swpa4-GFP fusion protein and POD activity assays in the apoplastic washing Xuid revealed that the swpa4 protein is secreted into the apoplastic space. In addition, a signiWcantly enhanced tolerance to a variety of abiotic and biotic stresses occurred in the transgenic plants. These plants harbored increased lignin and phenolic content, and H 2 O 2 was also generated under normal conditions. Furthermore, they showed an increased expression level of a variety of apoplastic acidic pathogenesis-related (PR) genes following enhanced H 2 O 2 production. These results suggest that the expression of swpa4 in the apoplastic space may function as a positive defense signal in the H 2 O 2 -regulated stress response signaling pathway.

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“…Investigations to understand the function of each POD in terms of various biotic and abiotic stresses helped to identify a stress-induced promoter (Jang et al 2004). The presence or absence of isoenzymes in response to stress showed that they are likely involved in a defence mechanism (Jang et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The presence or absence of isoenzymes in response to stress showed that they are likely involved in a defence mechanism (Jang et al 2004). In the present study, the presence of some isoenzymes at low irradiance and absence at high irradiance and conversely showed that some specific POD isoenzymes were involved in light stress in chestnut oak seedlings.…”

Section: Discussionmentioning

confidence: 99%

Effects of different irradiance levels on peroxidase activities in Quercus castaneifolia C.A. Mey. seedlings from different provenances

Babaei¹,

Jalali²,

Sohrabi³

et al. 2016

J. For. Sci.

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ABSTRACT:To understand the function of peroxidase (POD) in relation to a light gradient, changes in POD activity were studied in five different provenances of chestnut oak seedlings. An experiment was conducted in controlled conditions and six different irradiances (10, 20, 30, 50, 60, and 70% of full light) were considered. According to the results, POD activity was strongly related to irradiance and showed a decreasing response to light, with the largest changes at low light (10 to 30%) and a levelling-off at high light (50-70%). Five provenances were also significantly distinguished at 10-30% of full light. The gel analysis showed that there were several different bands between irradiances from 10 to 70% regardless of provenances. One isoenzyme with the highest electrophoretic mobility was significantly increased in response to reduced light and slightly decreased at high irradiance. Other isoenzymes were significantly increased at high irradiance, suggesting that these isoenzymes are most likely involved in response to light stress and leaf adaptation to high irradiance.

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Differential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, Pectobacterium chrysanthemi

Cited by 39 publications

References 28 publications

Changes in activities of antioxidant enzymes and their gene expression during leaf development of sweetpotato

Changes in activities of antioxidant enzymes and their gene expression during leaf development of sweetpotato

Overexpression of sweetpotato swpa4 peroxidase results in increased hydrogen peroxide production and enhances stress tolerance in tobacco

Effects of different irradiance levels on peroxidase activities in Quercus castaneifolia C.A. Mey. seedlings from different provenances

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