Differential expression changes in K562 cells during the hemin-induced erythroid differentiation and the phorbol myristate acetate (PMA)-induced megakaryocytic differentiation

Abstract

K562 cell line has been used as a model of common progenitor of erythroblasts and magakaryocytes and can be differentiated into erythroid and megakaryocytic lineages by hemin and phorbol myristate acetate (PMA) respectively. We analyzed mRNA expression in un-induced, hemin-induced and PMA-induced K562 cells by differential display reverse transcription polymerase chain reaction (DDRT-PCR) method. 314 differential expression sequence tags (ESTs) were obtained. Among them, 201 ESTs displayed up-regulation and 85 ESTs down-regulation after hemin induction, 186 ESTs showed up-regulation and 72 ESTs down-regulation after PMA induction. The differentially expressed genes included those encoding transcription factors, signaling factors, apoptosis-associated factors and others. 45 of these ESTs stand for genes whose open reading frames were found but whose functions remain unknown. 4 ESTs represent possibly new genes. Furthermore we compared differences of gene expression during hemin-induced erythroid differentiation and PMA-induced megakaryocytic differentiation and found that the expressional changes of some transcription factors and metabolism proteins are the common but the expressional changes of some signal pathways in these two differentiation processes are different. These results suggested that erythroid differentiation and megakaryocytic differentiation are associated in activation and repression of different signal pathways.

Induction of anion exchanger-1 translation and its opposite roles in the carcinogenesis of gastric cancer cells and differentiation of K562 cells

“…Therefore, differentiation of K562 cells has served as a representative model of studies of erythroid differentiation for many years (Huo et al, 2006;Wang et al, 2009). The undetectable expression of AE1 in K562 cells suggests that miR-24 may take part in the tumorigenesis of erythroleukemia by arresting erythroid maturation through silencing of AE1 expression.…”

The Effect of Hemin and Deferoxamine on Selenium, Zinc, and Iron Levels of K562 Cells

“…These processes are associated with a series of molecular events. The molecular events that govern these processes are only partially understood [34]. The function of erythrocyte is oxygen delivery.…”

“…Obtaining synchronized cells from long-term cultured proerythroblast cells is very difficult which led us to use K562 cells, which were suitable for this study. The K562 cell line has been used as a model of common progenitor of erythroblasts and megakaryocytes and can be differentiated into erythroid and megakaryocytic lineages by hemin (ferriprotoporphyrin IX) and phorbol myristate acetate (PMA) respectively [34].…”

Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells

“…Hemin chloride 40 uM (H-5533 Sigma; St Louis, MO, USA) and theophylline 7 mM (T-1633 Sigma; St Louis, MO, USA) were used as positive differentiation inducers for K-562 (Huo et al, 2006) and Daudi (Sandlund et al, 1993) cell lines, respectively. Triplicates of cell suspension of each cell line (10 6 cell/mL) in complete RMPI 1640 medium containing ARF solution to give final concentrations ranging from 0.1 to 100 µg/mL, or the required concentration of the cell differentiation inducers, in a 1000 µL final volume/well were distributed in 24-well culture plates (TPP; Switzerland) and incubated for 7 days at 37 °C in 5 % CO 2 .…”