Differential expression and regulation of the retinoblastoma family of proteins during testicular development and spermatogenesis: roles in the control of germ cell proliferation, differentiation and apoptosis

Yan, Wei; Kero, Jukka; Suominen, J.; Toppari, Jorma

doi:10.1038/sj.onc.1204254

Cited by 38 publications

(28 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly to tumorigenesis, spermatogenesis is a dynamic and complex process involved in massive cell proliferation and differentiation. Normal spermatogenesis is highly dependent on well-balanced germ cell proliferation, differentiation, and apoptosis (Yan et al 2001). The control of apoptosis is especially important.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of glucose-regulated protein 78 during spermatogenesis

Huo

Zhu

et al. 2004

Cell and Tissue Research

View full text Add to dashboard Cite

The aim of the present study was to isolate and identify proteins involved in the process of spermatogenesis. To achieve this goal we used the technique of proteomic analysis. Comparison of testis protein patterns obtained by high-resolution two-dimensional gel electrophoresis from 1-week- and 7-week-old mice showed significant differences in protein spot intensities. Subsequently several of these variant protein spots were identified by mass spectrometry. Glucose-regulated protein (GRP) 78 (Bip) was one of them. GRP78, expressed at a lower level in 1-week-old mouse testes compared to 7-week-old mouse testes, is a member of the heat shock 70 protein family. It has recently been shown to be important for protecting cells from apoptosis in somatic cells, especially in progressively growing tumor cells. Further, immunohistochemical (IHC) analyses of mouse and human testes sections were performed to determine the cellular distribution of this protein. A strong GRP78 staining was seen beginning with pachytene spermatocytes. These findings suggested that GRP78 might perform an important function in the process of spermatogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential expression of glucose-regulated protein 78 during spermatogenesis

Huo

Zhu

et al. 2004

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…Hyperphosphorylated states of p107 correlate with cell cycle progression while hypophosphorylated and reduced levels are associated with suppression of proliferation and apoptosis of germ cells. Severely reduced levels of pRb correlate with spermatogonial apoptosis Yan et al, 1997Yan et al, , 2001). …”

Section: Transcription Factorsmentioning

confidence: 97%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

View full text Add to dashboard Cite

As germ cells divide and differentiate from spermatogonia to spermatozoa, they share a number of structural and functional features that are common to all generations of germ cells and these features are discussed herein. Germ cells are linked to one another by large intercellular bridges which serve to move molecules and even large organelles from the cytoplasm of one cell to another. Mitochondria take on different shapes and features and topographical arrangements to accommodate their specific needs during spermatogenesis. The nuclear envelope and pore complex also undergo extensive modifications concomitant with the development of germ cell generations. Apoptosis is an event that is normally triggered by germ cells and involves many proteins. It occurs to limit the germ cell pool and acts as a quality control mechanism. The ubiquitin pathway comprises enzymes that ubiquitinate as well as deubiquitinate target proteins and this pathway is present and functional in germ cells. Germ cells express many proteins involved in water balance and pH control as well as voltage-gated ion channel movement. In the nucleus, proteins undergo epigenetic modifications which include methylation, acetylation, and phosphorylation, with each of these modifications signaling changes in chromatin structure. Germ cells contain specialized transcription complexes that coordinate the differentiation program of spermatogenesis, and there are many male germ cell-specific differences in the components of this machinery. All of the above features of germ cells will be discussed along with the specific proteins/genes and abnormalities to fertility related to each topic.

show abstract

“…Bouin's-fixed testis sections (5 m) were used for in situ hybridization and autoradiography as described (14). Northern blot analysis was performed as described (15).…”

Section: Methodsmentioning

confidence: 99%

Previously uncharacterized roles of platelet-activating factor acetylhydrolase 1b complex in mouse spermatogenesis

Yan

Assadi

Wynshaw‐Boris

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Platelet-activating factor (PAF) has been shown to affect sperm motility and acrosomal function, thereby altering fertility. PAF acetylhydrolase 1b (PAFAH1B) hydrolyzes PAF and is composed of three subunits [the lissencephaly (LIS1) protein and ␣1 and ␣2 subunits] and structurally resembles a GTP-hydrolyzing protein.Besides the brain, transcripts for Lis1, ␣1, and ␣2 are localized to meiotic and early haploid germ cells. Here, we report disruptions of the ␣2 (Pafah1b2) and ␣1 (Pafah1b3) genes in mice. Male mice homozygous null for ␣2 (␣2 ؊/؊ ) are infertile, and spermatogenesis is disrupted at mid-or late pachytene stages of meiosis or early spermiogenesis. Whereas mice homozygous mutant for ␣1 (␣1 ؊/؊ ) have normal fertility and normal spermatogenesis, those with disruptions of both ␣1 and ␣2 (␣1 ؊/؊ ␣2 ؊/؊ ) manifest an earlier disturbance of spermatogenesis with an onset at preleptotene or leptotene stages of meiosis. Testicular Lis1 protein levels are up-regulated in the ␣2 ؊/؊ and ␣1 ؊/؊ ␣2 ؊/؊ mice. Lowering Lis1 levels by inactivating one allele of Lis1 in ␣2 null or ␣1͞␣ 2 null genetic backgrounds (i.e., ␣2 ؊/؊ Lis1 ؉/؊ or ␣1 ؊/؊ ␣2 ؊/؊ Lis1 ؉/؊ mice) restored spermatogenesis and male fertility. Our data provide evidence for unique roles of the PAFAH1B complex and, particularly, the lissencephaly protein Lis1 in spermatogenesis.testis ͉ apoptosis ͉ lissencephaly

show abstract

Differential expression and regulation of the retinoblastoma family of proteins during testicular development and spermatogenesis: roles in the control of germ cell proliferation, differentiation and apoptosis

Cited by 38 publications

References 46 publications

Differential expression of glucose-regulated protein 78 during spermatogenesis

Differential expression of glucose-regulated protein 78 during spermatogenesis

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Previously uncharacterized roles of platelet-activating factor acetylhydrolase 1b complex in mouse spermatogenesis

Contact Info

Product

Resources

About