Differential expression and localisation of connexin-37 and connexin-43 in follicles of different stages in the 4-week-old mouse ovary

Teilmann, Stefan C.

doi:10.1016/j.mce.2004.10.014

Cited by 73 publications

(55 citation statements)

References 40 publications

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“…They have shown that mouse COCs do not need Cx43 to grow to developmentally competent cells, but Cx37 seems to be necessary for proper communication between surrounding cumulus-granulosa cells and oocytes during normal oogenesis. However, as opposed to that and in agreement with our results, Teilmann (2005) found in mice COCs that Cx43 may be translocated between cumulus-granulosa cells and oocyte in follicles during the process of preantral to antral transition. Furthermore, the localisation of Cx43 within the zona pellucida suggests its trans-zonal projection, which would point to new intracellular communications between somatic and germ cells during early folliculogenesis.…”

Section: A B C Dsupporting

confidence: 89%

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Kempisty

Ziółkowska

Piotrowska

et al. 2014

Acta Veterinaria Hungarica

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It is recognised that connexin 43 (Cx43) and cyclin-dependent kinase 4 (Cdk4) are involved in the cumulus cell-oocyte communication via gap junctions and the control of cell cycle progress. However, little is known about their mRNA expression pattern and encoded proteins distribution in porcine oocytes during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were collected from 31 puberal crossbred Landrace gilts and analysed for their Cdk4 and Cx43 mRNA expression using RQ-PCR and for the respective protein expression by confocal microscopic observations. An increased Cdk4 and Cx43 mRNA expression was found in oocytes after IVM (P < 0.001 and P < 0.05, respectively). Confocal microscopic observations revealed a significant increase of Cdk4 protein expression in the cytoplasm of oocytes during the maturation process. The localisation of Cx43 changed from zona pellucida before to cytoplasm of oocytes after IVM. It is supposed that the increased expression of Cdk4 and Cx43 mRNA in oocytes after IVM is linked with the accumulation of a large amount of templates during the process of oocyte maturation. The translocation especially of Cx43 from the zona pellucida into the cytoplasm may be associated with a decrease in gap junction activity in fully grown porcine oocytes. Both Cdk4 and Cx43 can be used as 'checkpoints' of oocyte maturation.

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Section: A B C Dsupporting

confidence: 89%

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Kempisty

Ziółkowska

Piotrowska

et al. 2014

Acta Veterinaria Hungarica

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“…In addition to paracrine signaling, direct cell-to-cell communication via gap junctions also plays a significant role in pre-antral growth, as evident from mice with deficiencies of connexin43 (CX43) and connexin37 (CX37), two of the core proteins that form the gap junctions in the ovary (Granot and Dekel 1994;Teilmann 2005). In CX43-deficient ovaries, follicles fail to develop beyond the primary stage (Ackert et al 2001), whereas CX37-deficient mice fail to develop mature (Graafian) follicles (Simon et al 1997).…”

Section: Tgf-β Superfamily and Their Role In Pre-antral Folliculogenesismentioning

confidence: 99%

Ovarian Folliculogenesis

Rimon-Dahari¹,

Yerushalmi-Heinemann²,

Alyagor³

et al. 2016

Results and Problems in Cell Differentiation

186

111

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Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showed that the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended. Abstrak Gosipol adalah zat yang terkandung dalam ekstrak biji kapas dan diduga mempunyai kemampuan antifertilitas sehingga sering digunakan sebagai bahan herbal kontrasepsi. Penelitian ini bertujuan mengkaji gambaran folikulogenesis pada mencit yang diberi ekstrak biji kapas. Sebanyak 60 ekor induk mencit betina strain DDY berumur 14-15 minggu dengan bobot badan 30-35 g dibagi menjadi 4 kelompok dan diberi ekstrak biji kapas masing-masing 0; 1,5; 2,1 dan 2,7 g/kg BB selama 5, 10, 15, 24 dan 24 + 10 hari (tanpa pemberian ekstrak biji kapas). Pada setiap akhir waktu pengamatan, mencit di-euthanasia untuk mengamati histomorfologi perkembangan folikel (masing-masing tiga ekor dari tiap perlakuan). Status estrus mencit dievaluasi berdasarkan gambaran sel dari ulas vagina dengan pewarnaan Giemsa. Hasil penelitian menunjukkan bahwa jumlah folikel rendah (P < 0,05) dibandingkan dengan kontrol setelah 5 hari pemberian ekstrak biji kapas dengan dosis 2,7 g/kg BB yaitu 23 ± 3,6. Pada dosis 1,5 dan 2,1 g/kg BB jumlah folikel menjadi rendah setelah 24 hari yaitu 25 ± 10,4; 27 ± 3,5. Efek pemulihan jumlah folikel setelah pemberian ekstrak biji kapas selama 24 hari paling baik pada dosis 1,5 g/kg BB. Pemanjangan siklus estrus terjadi pada mencit yang diberi ekstrak bji kapas pada semua dosis perlakuan. Berdasarkan hasil tersebut dapat disimpulkan bahwa walaupun terjadi penurunan jumlah folikel yang berkembang dan terjadi pemanjangan siklus estrus setelah pemberian ekstrak biji kapas, tetapi efek tersebut bersifat reversible setelah pe...

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“…p70 S6 kinase, which phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 0 -oligopyrimidine tract mRNAs (Manning 2004), is phosphorylated during the transition from primary to secondary follicles, presumably serving to promote the protein synthesis required for subsequent oocyte growth (unpublished data). Connexin-43, which is required for gap-junction formation and communication between granulosa cells Specificity of the Foxo3 phenotype (Ackert et al 2001, Teilmann 2005, is first detectable in primary follicles, and its levels continue to increase during the progression to the secondary and antral stages. For all of these markers, the timing of these changes in expression was identical, stage-for-stage, in Foxo3 C/C and Foxo3 K/K ovaries (Fig.…”

Section: Foxo3mentioning

confidence: 99%

Specificity of the requirement for Foxo3 in primordial follicle activation

John¹,

Shirley²,

Gallardo³

et al. 2007

Reproduction

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Primordial follicles are long-lived structures assembled early in life. The mechanisms that control the balance between the conservation and the activation of primordial follicles are critically important for fertility and dictate the onset of menopause. The forkhead transcription factor Foxo3 serves an essential role in these processes by suppressing the growth of primordial follicles, thereby preserving them until later in life. While other factors regulating primordial follicle growth have been described, most serve multiple functions at several stages of female germ cell or follicle development, and corresponding mouse mutants exhibit pleiotropic phenotypes with disruption of multiple stages of follicle assembly, development, or survival. To investigate the possibility that Foxo3 also functions in other aspects of ovarian development beyond its known role in primordial follicle activation (PFA), we performed detailed analyses of mouse ovaries including electron microscopy to study primordial follicle structure, assembly, and early growth. These analyses revealed that the timing of primordial follicle assembly, early oocyte survival, and the expression of early germ line markers were unaffected in early Foxo3 ovaries. Taken together, these studies demonstrate that the phenotype associated with Foxo3 deficiency is remarkably specific for PFA and further support the placement of Foxo3 in a unique phenotypic class among mammalian female sterile mutants. Lastly, we discuss the implications of the specificity of this mutant phenotype with regard to the hypothesis that oocyte regeneration may occur in adults and serves as a means to replenish oocytes lost via natural physiological processes.

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Differential expression and localisation of connexin-37 and connexin-43 in follicles of different stages in the 4-week-old mouse ovary

Cited by 73 publications

References 40 publications

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Ovarian Folliculogenesis

Specificity of the requirement for Foxo3 in primordial follicle activation

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