Differential estrogen-related responses in myofiber cross-sectional area of pelvic floor muscles in female rats

Carrasco-Ruiz, Ángeles; Sánchez-García, Octavio; Pacheco, Pablo; Martı́nez–Gómez, Margarita; Cuevas-Romero, Estela; Castelán, Francisco

doi:10.1080/09513590.2021.1875427

Cited by 3 publications

(5 citation statements)

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“…Serum estradiol levels and the age of rabbits could explain the CSA response herein and in the previous study. Otherwise, findings herein, and from other rat studies, 27,28 confirm estrogen can differentially affect individual PFM. Although, in rats, the Pcm myofiber CSA seems more responsive to estrogens that induce a CSA reduction 27,28 .…”

Section: Discussionsupporting

confidence: 61%

“…Otherwise, findings herein, and from other rat studies, 27,28 confirm estrogen can differentially affect individual PFM. Although, in rats, the Pcm myofiber CSA seems more responsive to estrogens that induce a CSA reduction 27,28 . Overall, we believe these findings shed light on the understanding of menopausal hormone therapy on PFM morphometry 29 …”

Section: Discussionsupporting

confidence: 61%

“…Although, in rats, the Pcm myofiber CSA seems more responsive to estrogens that induce a CSA reduction. 27,28 Overall, we believe these findings shed light on the understanding of menopausal hormone therapy on PFM morphometry. 29 Reduced estradiol levels undermine bladder contractility in rabbits.…”

Section: Discussionmentioning

confidence: 65%

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Estrogens influence differentially on the pelvic floor muscles activation at somatovisceral reflexes involved in micturition of rabbits

et al. 2021

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Objective: To determine the estrogen-dependency of the bladder and urethral function and the coordinated activation of pelvic floor muscles (PFM) during micturition.Methods: We allocated age-matched female rabbits to control, 1-month ovariectomized (OVX), and OVX plus 2-week estradiol benzoate (EB) groups to record cystometry, urethral pressure, and electromyograms of bulbospongiosus (Bsm), and pubococcygeus muscles (Pcm) simultaneously. We also measured serum estradiol levels and myofiber cross-sectional area. We assessed urodynamic and urethral variables, categorized the Bsm-Pcm activation patterns at storage and voiding phases, and obtained the power spectrum density of muscle activation around the voiding phase. We investigated the influence of ovarian hormones, in general, and the contribution of estrogen, particularly on the functions of the bladder, urethra, and PFM. Statistical significance was set at P < 0.05.Results: Ovarian hormones influence the bladder, urethral, and PFM functions. The urodynamics analyses indicated estrogens contribute to voiding duration and, to a lesser extent, to the time between bladder contractions. Urethral pressure at closure (maximal pressure-to-maximal urethral pressure ratio) improved partially (8%, P < 0.05) in the OVX plus 2-week estradiol benzoate compared with OVX, but urethral resistance increased ($1.9-fold, P < 0.05) compared with control rabbits. Our findings support that Pcm activity at voiding is estrogensensitive, albeit EB administration reduced it at storage resume, which relates to high urethral resistance.Conclusions: Ovariectomy impairs bladder and urethral pressures and Bsm and Pcm activation at micturition in anesthetized rabbits. Estrogen administration partially reverts some of these effects and influences Pcm activation.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionsupporting

confidence: 61%

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Estrogens influence differentially on the pelvic floor muscles activation at somatovisceral reflexes involved in micturition of rabbits

et al. 2021

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“…Diminished genital blood flow is a common physiological factor in FSD 28 and is a side effect of ovariectomizing female rats. 16 Hormone priming has been shown to maintain vaginal and pelvic floor muscle health after ovariectomy, 29,30 which should have yielded consistent muscle health across H+/H++ animal groups in Experiment 2 but may have contributed to variability in Experiment 1 animals. Tibial nerve stimulation has been shown to increase vaginal blood flow 10 but not vulvar blood flow 11 in anesthetized rats.…”

Section: Discussionmentioning

confidence: 99%

“…An increase in hormone production in response to nerve stimulation at other locations has been documented 33,35 and could explain the increase in sexually receptive behavior over time that we observed here. The vaginal and pelvic floor structural integrity can be examined in a future study 29,30 to determine if stimulation has beneficial effects at different hormone levels. The cortical effects of stimulation can also be studied by looking at c-fos activation in the estrogen-containing regions of the brain in response to tibial stimulation.…”

Section: Discussionmentioning

confidence: 99%

Immediate and Long-Term Effects of Tibial Nerve Stimulation on the Sexual Behavior of Female Rats

Zimmerman

Mentzelopoulos

Parrish

et al. 2022

Preprint

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Objectives: There are limited treatment options for female sexual dysfunction (FSD). Percutaneous tibial nerve stimulation (PTNS) has shown improvements in FSD symptoms in neuromodulation clinical studies, but the direct effects on sexual function are not understood. This study evaluated the immediate and long-term effects of PTNS on sexual motivation and receptivity in a rat model of menopausal women. Our primary hypothesis was that long-term PTNS would yield greater changes in sexual behavior than short-term stimulation. Materials and Methods: In two Experiments, after receiving treatment, we placed ovariectomized female rats in an operant chamber apparatus with two compartments: a tethered, sexually active male was on one compartment and the female's access to the male's compartment was controlled by nose poking. We used five treatment conditions, which were with or without PTNS and no, partial, or full hormone priming. In Experiment 1, we rotated rats through each treatment condition twice with behavioral testing immediately following treatment for 10 weeks. In Experiment 2, we committed rats to one treatment condition for 6 weeks and sexual behavior was tracked over time. We quantified sexual motivation through number, latency, and frequency of nose pokes as well as completed intervals. We quantified sexual receptivity through mounts, lordosis quotient, and time spent in chamber areas. Results: No primary comparisons were significant in this study. In Experiment 1, we observed increased sexual motivation immediately following PTNS with hormones, but not receptivity. In Experiment 2, we observed trends of increased sexual receptivity and some sexual motivation metrics when PTNS was applied long-term with hormones. Conclusions: PTNS combined with hormone priming shows potential for increasing sexual motivation in the short-term and sexual receptivity in the long-term in rats. Further studies are needed to examine variability in rat behavior and to investigate PTNS as a treatment for FSD in menopausal women.

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Immediate and Long-Term Effects of Tibial Nerve Stimulation on the Sexual Behavior of Female Rats

Zimmerman

Mentzelopoulos

Parrish

et al. 2024

Neuromodulation: Technology at the Neural Interface

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Differential estrogen-related responses in myofiber cross-sectional area of pelvic floor muscles in female rats

Cited by 3 publications

References 32 publications

Estrogens influence differentially on the pelvic floor muscles activation at somatovisceral reflexes involved in micturition of rabbits

Estrogens influence differentially on the pelvic floor muscles activation at somatovisceral reflexes involved in micturition of rabbits

Immediate and Long-Term Effects of Tibial Nerve Stimulation on the Sexual Behavior of Female Rats

Immediate and Long-Term Effects of Tibial Nerve Stimulation on the Sexual Behavior of Female Rats

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