Differential estrogen receptor gene expression in human peripheral blood mononuclear cell populations

Phiel, Kristen L.; Henderson, Ruth A.; Adelman, Steven J.; Elloso, M. Merle

doi:10.1016/j.imlet.2004.10.007

Cited by 297 publications

(259 citation statements)

References 47 publications

(51 reference statements)

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“…In accordance with previous reports, we confirmed that both ER mRNA isoforms are constitutively expressed in human peripheral blood monocytes ( Fig. 2A) (16,17).…”

Section: Er Expression In Primary Human Monocytessupporting

confidence: 93%

Estradiol Attenuates Lipopolysaccharide-Induced CXC Chemokine Ligand 8 Production by Human Peripheral Blood Monocytes

Pioli

Jensen

Weaver

et al. 2007

The Journal of Immunology

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Regulation of the inflammatory response is imperative to the maintenance of immune homeostasis. Activated monocytes elaborate a broad variety of proinflammatory cytokines that mediate inflammation, including CXCL8. Release of this chemokine attracts neutrophils to sites of bacterial invasion and inflammation; however, high levels of CXCL8 may result in excessive neutrophil infiltration and subsequent tissue damage. In this study, we demonstrate that 17β-estradiol (E2) attenuates LPS-induced expression of CXCL8 in human peripheral blood monocytes. Treatment of monocytes with estradiol before administration of LPS reduces CXCL8 message and protein production through an estrogen receptor-dependent mechanism, and luciferase reporter assays demonstrate that this inhibition is mediated transcriptionally. Importantly, the ability of estradiol-pretreated LPS-activated monocytes to mobilize neutrophils is impaired. These results implicate a role for estradiol in the modulation of the immune response, and may lead to an enhanced understanding of gender-based differences in inflammatory control mechanisms.

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“…In accordance with previous reports, we confirmed that both ER mRNA isoforms are constitutively expressed in human peripheral blood monocytes ( Fig. 2A) (16,17).…”

Section: Er Expression In Primary Human Monocytessupporting

confidence: 93%

Estradiol Attenuates Lipopolysaccharide-Induced CXC Chemokine Ligand 8 Production by Human Peripheral Blood Monocytes

Pioli

Jensen

Weaver

et al. 2007

The Journal of Immunology

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“…ERα and ER are both subtypes of ER, and they are different in construction in both N end and C end, which imply they may have different functions. ER and ERβ have been reported to be co-expressed on human T lymphocyte (8,9), and our study confirmed their coexpression. There are different conclusions about which ER subtype is dominantly expressed on human T lymphocytes and which subtype is mainly responsible for the effect of estrogen.…”

Section: Discussionsupporting

confidence: 86%

“…Several reports have addressed the expressions of two estrogen receptors (ERs), ER and ER, on human T lymphocyte (8,9). However, little is known regarding the relation of ERT, ER expression on T lymphocytes and apoptosis of T lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Transdermal Estrogen Therapy on Expressions of Estrogen Receptors and T-lymphocyte Apoptosis in Surgically Menopausal Women

et al. 2009

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Studies have demonstrated estrogen replacement therapy can improve the life quality of surgically menopausal women. However, the mechanisms in this process remain poorly defined. Here we show the effect of transdermal estrogen therapy on expressions of estrogen receptors and T-lymphocyte apoptosis in surgically postmenopausal women. Fifteen surgically menopausal women, 15 naturally menopausal women and 15 young women were chosen in our studies. Peripheral vein blood was collected and serum E 2 and FSH levels were assessed using ACCESS. T-lymphocyte apoptosis and the expressions of Fas, FasL and ER subtypes  and  were determined. The serum E 2 levels of surgically menopausal woman were significantly higher, and the "Improved Kupperman Index" and the scores of "Menopause Specific Quality of Life Questionnaire" in surgically menopausal women were significantly low after ERT. The rates of T-lymphocyte apoptosis and FasL expression in surgically menopausal women were decreased after ERT, but the difference was not significant. The expressions of ERα and ERβ in two menopausal groups were significantly lower than those of the young group. They were both significantly up-regulated after 3 months of ERT. Transdermal ERT could significantly upregulate the serum E 2 level, could improve menopausal symptoms and life quality of surgically menopausal women and upregulate ERα and ERβ expressions on T lymphocytes, especially ERβ. Thus, the low dose of transdermal ERT may have a protective effect on menopausal women's immune function and aging. Cellular & Molecular Immunology. 2009;6(4):277-283.

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“…In a PCR work, CD4 + T cells were reported to express relatively high levels of ERa mRNA as compared with ERb, and B and NK cells were also reported to express ERa and ERb (20,21). It has been demonstrated that 17b-estradiol suppresses production of IL-2, IL-6, TNF-a, IL-1 receptor antagonist, and IL-1b from human peripheral blood T cells (22,23).…”

Section: Discussionmentioning

confidence: 99%

Fluctuation of Peripheral Blood T, B, and NK Cells during a Menstrual Cycle of Normal Healthy Women

Lee¹,

Kim²,

Jang³

et al. 2010

The Journal of Immunology

106

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Cyclical hormonal changes during an ovarian cycle may affect immune responses, which is crucial for the embryonic implantation. We aim to investigate whether the levels and activity of T, B, and NK cells change during a menstrual cycle. Twenty-two normally cycling women were enrolled and peripheral blood was drawn serially during a menstrual cycle. Intracellular cytokine expression of CD3+CD4+ and CD3+CD8+ cells, and Th1/Th2 cytokine-producing T cell ratios were determined using flow cytometric analysis. NK cell cytotoxicity was measured by flow cytometric analysis at E:T ratios of 50:1, 25:1, and 12.5:1 and also using LU at 20%. Proportions (percentage) of CD3+ (p = 0.046) and CD3+CD4+ (p = 0.002) T cells were increased in the follicular phase compared with the luteal phase. The levels of CD3−CD56+ (p = 0.010) and CD3−CD56dim (p = 0.012) NK cells and NK cytotoxicity at E:T ratio of 50:1, 25:1, and 12.5:1 and LU at 20% were significantly increased in the luteal phase compared with the follicular phase. Even though IL-10–producing CD3+CD4+ T cells were significantly lower in the midluteal phase as compared with the early follicular phase, proportions of CD19+ B cells, CD3+CD56+ NKT cells, Th1 cytokine-producing T cell subsets, and ratios of Th1/Th2 cytokine-producing T cells were not significantly changed during a menstrual cycle. We conclude that peripheral blood NK and T cell levels as well as NK cytotoxicity are changed during a menstrual cycle. Neuroendocrine regulation on immune responses is suggested during an ovarian cycle, which may be critical for embryonic implantation and pregnancy.

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Differential estrogen receptor gene expression in human peripheral blood mononuclear cell populations

Cited by 297 publications

References 47 publications

Estradiol Attenuates Lipopolysaccharide-Induced CXC Chemokine Ligand 8 Production by Human Peripheral Blood Monocytes

Estradiol Attenuates Lipopolysaccharide-Induced CXC Chemokine Ligand 8 Production by Human Peripheral Blood Monocytes

Effects of Transdermal Estrogen Therapy on Expressions of Estrogen Receptors and T-lymphocyte Apoptosis in Surgically Menopausal Women

Fluctuation of Peripheral Blood T, B, and NK Cells during a Menstrual Cycle of Normal Healthy Women

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