Differential epidemiology and antibiotic resistance of lactose-fermenting and non-fermenting Escherichia coli: Is it just a matter of taste?

Gajdács, Márió; Ábrók, Marianna; Lázár, Andrea; Burián, Katalin

doi:10.1007/s42977-020-00016-6

Cited by 12 publications

(10 citation statements)

References 44 publications

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“…Nevertheless, in another study by Gajdács et al, the antibiotic susceptibilities of lactose-fermenting and non-fermenting E. coli from UTIs were compared during a 5-year study period in Southern Hungary. In this study, it was found that the resistance rates of lactose non-fermenters were higher for fluoroquinolones, fosfomycin and nitrofurantoin in both inpatients and outpatients [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Association between Biofilm-Production and Antibiotic Resistance in Uropathogenic Escherichia coli (UPEC): An In Vitro Study

2020

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Urinary tract infections (UTIs) are among the most common infections requiring medical attention worldwide. The production of biofilms is an important step in UTIs, not only from a mechanistic point of view, but this may also confer additional resistance, distinct from other aspects of multidrug resistance (MDR). A total of two hundred and fifty (n = 250) Escherichia coli isolates, originating from clean-catch urine samples, were included in this study. The isolates were classified into five groups: wild-type, ciprofloxacin-resistant, fosfomycin-resistant, trimethoprim-sulfamethoxazole-resistant and extended spectrum β-lactamase (ESBL)-producing strains. The bacterial specimens were cultured using eosine methylene blue agar and the colony morphology of isolates were recorded. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disk diffusion method and E-tests. Biofilm-formation of the isolates was carried out with the crystal violet tube-adherence method. n = 76 isolates (30.4%) produced large colonies (>3 mm), mucoid variant colonies were produced in n = 135 cases (54.0%), and n = 119 (47.6%) were positive for biofilm formation. The agreement (i.e., predictive value) of mucoid variant colonies in regard to biofilm production in the tube-adherence assay was 0.881 overall. Significant variation was seen in the case of the group of ESBL-producers in the ratio of biofilm-producing isolates. The relationship between biofilm-production and other resistance determinants has been extensively studied. However, no definite conclusion can be reached from the currently available data.

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Section: Discussionmentioning

confidence: 99%

Association between Biofilm-Production and Antibiotic Resistance in Uropathogenic Escherichia coli (UPEC): An In Vitro Study

2020

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“…The morning midstream urine and urine from the urinary catheter were collected from patients under complete aseptic conditions and cultured on blood and MacConkey agars (Oxoid Ltd., England) to detect different E. coli strains. 15 The isolated UPEC was confirmed by Gram staining and the standard biochemical tests and only one isolate from each patient was investigated. 16 The UTI was confirmed by isolation of >10 5 colony-forming units (CFU) UPEC/mL midstream urine and ≥10 3 CFU UPEC/mL catheterized urine.…”

Section: Methodsmentioning

confidence: 99%

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

El-Mahdy

Mahmoud

Shrief

2021

IDR

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Purpose Characterization of different uropathogenic E. coli (UPEC) phylogroups is crucial to understand pathogenesis of urinary tract infection (UTI). The objective of our study was to evaluate the antibiotic resistance pattern, biofilm formation and pathogenicity islands (PAIs) of UPEC phylogroups isolated from catheter-associated UTI (CAUTI) compared to community UTI (Com-UTI). Patients and Methods This study included 90 UPEC strains recovered from CAUTI and Com-UTI. Antimicrobial susceptibility was tested by the Kirby–Bauer method and extended spectrum beta-lactamase (ESBL) production was confirmed using the combined disk. The biofilm formation was tested using the microtiter plate assay. Main E. coli phylogroups (A, B1, B2 and D) were detected by multiplex PCR and 2 multiplex PCR detected the 8 PAIs. Results Antibiotic resistance of UPEC strains showed a similar high resistance in CAUTI and Com-UTI. Isolates from CAUTI significantly produced biofilm higher than Com-UTI strains (68.9% vs 44.4%). In CAUTI and Com-UTI isolates, phylogroup A was the commonest (53.3% vs 48.9%, respectively). PAI IV536 was the most common in the strains from CAUTI (71.1%) and Com-UTI (73.3%). No significant relationship was detected between the studied characters and different phylogroups except the significant resistance to cefotaxime, ceftazidime and aztreonam among phylogroups from CAUTI isolates. Conclusion Increased antibiotic resistance and ESBLs were detected in UPEC strains from CAUTI and Com-UTI. The strains from CAUTI significantly produced biofilm higher than Com-UTI strains. Phylogroup A was the predominate phylogroup and PAI IV536 was the most prevalent marker in all phylogroups from both types of UTI.

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“…The MICs of colistin were determined by the broth microdilution in cation-adjusted Mueller-Hinton Broth (CAMHB). 23 The MIC breakpoint of colistin for E. coli was interpreted following the European Committee on Antimicrobial Susceptibility Testing (EUCAST 2019) 24 (susceptible, ≤2 mg/L; resistant, >2 mg/L). The E. coli ATCC 25,922 served as the quality control for susceptibility testing.…”

Section: Bacterial Strainsmentioning

confidence: 99%

<p>Resistance and Heteroresistance to Colistin in <em>Escherichia coli</em> Isolates from Wenzhou, China</p>

Liao¹,

Lin²,

Jia³

et al. 2020

IDR

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Background: Colistin is being administered as last-line therapy for patients that have failed to respond to other available antibiotics that are active against Escherichia coli. The underlying mechanisms of colistin resistance and heteroresistance remain largely uncharacterized. The present study investigated the mechanisms of resistance and heteroresistance to colistin in Escherichia coli isolates from Wenzhou, China. Materials and Methods: Colistin resistance was detected by the broth microdilution method (BMD). Colistin heteroresistance was determined by population analysis profiles (PAPs). The polymerase chain reaction (PCR) was conducted to detect mcr-1, mcr-2, mcr-3, pmrA, pmrB, phoP, phoQ and mgrB, and quantitative real-time PCR (qRT-PCR) was used to determine the expression levels of mcr-1, pmrC, pmrA and pmrB. Lipid A characterization was conducted by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Results: 0.69% (2/291) of Escherichia coli strains were resistant to colistin, whereas the heteroresistance rate reached 1.37% (4/291). mcr-1, the mobile colistin-resistance gene, was present in the two resistant isolates. The substitutions in PmrB were detected in the two heteroresistant isolates. The transcripts levels of the pmrCAB operon were upregulated in two of the heteroresistant isolates. carbonylcyanide m-chlorophenylhydrazone (CCCP) was able to reverse colistin resistance of all isolates tested and exhibited a significantly higher effect on colistin-heteroresistant isolates. MALDI-TOF MS indicated that the additional phosphoethanolamine (PEtn) moieties in lipid A profiles were present both in colistin-resistant and heteroresistant isolates. Conclusion: The present study was the first to investigate the differential mechanisms between colistin resistance and heteroresistance. The development of colistin heteroresistance should be addressed in future clinical surveillance.

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Differential epidemiology and antibiotic resistance of lactose-fermenting and non-fermenting Escherichia coli: Is it just a matter of taste?

Cited by 12 publications

References 44 publications

Association between Biofilm-Production and Antibiotic Resistance in Uropathogenic Escherichia coli (UPEC): An In Vitro Study

Association between Biofilm-Production and Antibiotic Resistance in Uropathogenic Escherichia coli (UPEC): An In Vitro Study

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

<p>Resistance and Heteroresistance to Colistin in <em>Escherichia coli</em> Isolates from Wenzhou, China</p>

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